Protein turnover of the cytosolic ribosome and proteasome and their biochemical changes in cells in response to oxidative stress

Karzan Jalal Salih

Research output: ThesisDoctoral Thesis

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Abstract

Protein turnover is crucial to maintain cellular function and development. Stable isotope labelling in Arabidopsis thaliana showed a slow degradation rate of most ribosomal (r) and proteasomal proteins but identified RACK1 and RPP0-like r-proteins with high degradation rates even under control conditions. Knockout of the RPP0-like rapidly degrading r-protein affected root growth. The degradation rates of most r-proteins were even slower following oxidative stress. Combining ribosome quantification and cellular protein synthesis rates showed ribosomes remained functional during oxidative stress. However, this stress caused changes in the abundance of specific r-protein isoforms and a faster degradation rate of the r-protein RPS14C.
Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • The University of Western Australia
Award date23 Apr 2019
DOIs
Publication statusUnpublished - 2019

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proteasome endopeptidase complex
ribosomes
protein metabolism
oxidative stress
degradation
proteins
cells
protein isoforms
isotope labeling
stable isotopes
root growth
Arabidopsis thaliana
protein synthesis

Cite this

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title = "Protein turnover of the cytosolic ribosome and proteasome and their biochemical changes in cells in response to oxidative stress",
abstract = "Protein turnover is crucial to maintain cellular function and development. Stable isotope labelling in Arabidopsis thaliana showed a slow degradation rate of most ribosomal (r) and proteasomal proteins but identified RACK1 and RPP0-like r-proteins with high degradation rates even under control conditions. Knockout of the RPP0-like rapidly degrading r-protein affected root growth. The degradation rates of most r-proteins were even slower following oxidative stress. Combining ribosome quantification and cellular protein synthesis rates showed ribosomes remained functional during oxidative stress. However, this stress caused changes in the abundance of specific r-protein isoforms and a faster degradation rate of the r-protein RPS14C.",
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school = "The University of Western Australia",

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AB - Protein turnover is crucial to maintain cellular function and development. Stable isotope labelling in Arabidopsis thaliana showed a slow degradation rate of most ribosomal (r) and proteasomal proteins but identified RACK1 and RPP0-like r-proteins with high degradation rates even under control conditions. Knockout of the RPP0-like rapidly degrading r-protein affected root growth. The degradation rates of most r-proteins were even slower following oxidative stress. Combining ribosome quantification and cellular protein synthesis rates showed ribosomes remained functional during oxidative stress. However, this stress caused changes in the abundance of specific r-protein isoforms and a faster degradation rate of the r-protein RPS14C.

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KW - Ribosomal protein turnover

KW - H202 and menadione treatment

KW - MRM technique

KW - 14C-leucine labelling

KW - 15N labelling

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DO - 10.26182/5cda29aa87292

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