Protein secretion and outer membrane assembly in Alphaproteobacteria

Xenia Gatsos, Andrew J. Perry, Khatira Anwari, Pavel Dolezal, P. Peter Wolynec, Vladimir A. Likic, Anthony W. Purcell, Susan K. Buchanan, Trevor Lithgow

Research output: Contribution to journalReview article

66 Citations (Scopus)

Abstract

The assembly of beta-barrel proteins into membranes is a fundamental process that is essential in Gram-negative bacteria, mitochondria and plastids. Our understanding of the mechanism of beta-barrel assembly is progressing from studies carried out in Escherichia coli and Neisseria meningitidis. Comparative sequence analysis suggests that while many components mediating beta-barrel protein assembly are conserved in all groups of bacteria with outer membranes, some components are notably absent. The Alphaproteobacteria in particular seem prone to gene loss and show the presence or absence of specific components mediating the assembly of beta-barrels: some components of the pathway appear to be missing from whole groups of bacteria (e.g. Skp, YfgL and NlpB), other proteins are conserved but are missing characteristic domains (e.g. SurA). This comparative analysis is also revealing important structural signatures that are vague unless multiple members from a protein family are considered as a group (e.g. tetratricopeptide repeat (TPR) motifs in YfiO, beta-propeller signatures in YfgL). Given that the process of the beta-barrel assembly is conserved, analysis of outer membrane biogenesis in Alphaproteobacteria, the bacterial group that gave rise to mitochondria, also promises insight into the assembly of beta-barrel proteins in eukaryotes.

Original languageEnglish
Pages (from-to)995-1009
Number of pages15
JournalFEMS Microbiology Reviews
Volume32
Issue number6
DOIs
Publication statusPublished - Nov 2008
Externally publishedYes

Cite this

Gatsos, X., Perry, A. J., Anwari, K., Dolezal, P., Wolynec, P. P., Likic, V. A., ... Lithgow, T. (2008). Protein secretion and outer membrane assembly in Alphaproteobacteria. FEMS Microbiology Reviews, 32(6), 995-1009. https://doi.org/10.1111/j.1574-6976.2008.00130.x
Gatsos, Xenia ; Perry, Andrew J. ; Anwari, Khatira ; Dolezal, Pavel ; Wolynec, P. Peter ; Likic, Vladimir A. ; Purcell, Anthony W. ; Buchanan, Susan K. ; Lithgow, Trevor. / Protein secretion and outer membrane assembly in Alphaproteobacteria. In: FEMS Microbiology Reviews. 2008 ; Vol. 32, No. 6. pp. 995-1009.
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Gatsos, X, Perry, AJ, Anwari, K, Dolezal, P, Wolynec, PP, Likic, VA, Purcell, AW, Buchanan, SK & Lithgow, T 2008, 'Protein secretion and outer membrane assembly in Alphaproteobacteria' FEMS Microbiology Reviews, vol. 32, no. 6, pp. 995-1009. https://doi.org/10.1111/j.1574-6976.2008.00130.x

Protein secretion and outer membrane assembly in Alphaproteobacteria. / Gatsos, Xenia; Perry, Andrew J.; Anwari, Khatira; Dolezal, Pavel; Wolynec, P. Peter; Likic, Vladimir A.; Purcell, Anthony W.; Buchanan, Susan K.; Lithgow, Trevor.

In: FEMS Microbiology Reviews, Vol. 32, No. 6, 11.2008, p. 995-1009.

Research output: Contribution to journalReview article

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T1 - Protein secretion and outer membrane assembly in Alphaproteobacteria

AU - Gatsos, Xenia

AU - Perry, Andrew J.

AU - Anwari, Khatira

AU - Dolezal, Pavel

AU - Wolynec, P. Peter

AU - Likic, Vladimir A.

AU - Purcell, Anthony W.

AU - Buchanan, Susan K.

AU - Lithgow, Trevor

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AB - The assembly of beta-barrel proteins into membranes is a fundamental process that is essential in Gram-negative bacteria, mitochondria and plastids. Our understanding of the mechanism of beta-barrel assembly is progressing from studies carried out in Escherichia coli and Neisseria meningitidis. Comparative sequence analysis suggests that while many components mediating beta-barrel protein assembly are conserved in all groups of bacteria with outer membranes, some components are notably absent. The Alphaproteobacteria in particular seem prone to gene loss and show the presence or absence of specific components mediating the assembly of beta-barrels: some components of the pathway appear to be missing from whole groups of bacteria (e.g. Skp, YfgL and NlpB), other proteins are conserved but are missing characteristic domains (e.g. SurA). This comparative analysis is also revealing important structural signatures that are vague unless multiple members from a protein family are considered as a group (e.g. tetratricopeptide repeat (TPR) motifs in YfiO, beta-propeller signatures in YfgL). Given that the process of the beta-barrel assembly is conserved, analysis of outer membrane biogenesis in Alphaproteobacteria, the bacterial group that gave rise to mitochondria, also promises insight into the assembly of beta-barrel proteins in eukaryotes.

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KW - membrane structure

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KW - Alphaproteobacteria

KW - mitochondria

KW - CARBOXY-TERMINAL PHENYLALANINE

KW - GRAM-NEGATIVE BACTERIA

KW - BETA-BARREL PROTEINS

KW - ESCHERICHIA-COLI

KW - CRYSTAL-STRUCTURE

KW - MOLECULAR CHAPERONE

KW - TREPONEMA-PALLIDUM

KW - STRUCTURAL BASIS

KW - EVOLUTIONARY CONSERVATION

KW - SACCHAROMYCES-CEREVISIAE

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DO - 10.1111/j.1574-6976.2008.00130.x

M3 - Review article

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EP - 1009

JO - FEMS Microbiology Reviews

JF - FEMS Microbiology Reviews

SN - 0168-6445

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Gatsos X, Perry AJ, Anwari K, Dolezal P, Wolynec PP, Likic VA et al. Protein secretion and outer membrane assembly in Alphaproteobacteria. FEMS Microbiology Reviews. 2008 Nov;32(6):995-1009. https://doi.org/10.1111/j.1574-6976.2008.00130.x