Protein import into plant mitochondria: Signals, machinery, processing, and regulation

Monika Murcha, B. Kmiec, S. Kubiszewski-Jakubiak, P.F. Teixeira, E.G. Glaser, J.M. Whelan

Research output: Contribution to journalReview article

44 Citations (Scopus)

Abstract

© The Author 2014. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. The majority of more than 1000 proteins present in mitochondria are imported from nuclear-encoded, cytosolically synthesized precursor proteins. This impressive feat of transport and sorting is achieved by the combined action of targeting signals on mitochondrial proteins and the mitochondrial protein import apparatus. The mitochondrial protein import apparatus is composed of a number of multi-subunit protein complexes that recognize, translocate, and assemble mitochondrial proteins into functional complexes. While the core subunits involved in mitochondrial protein import are well conserved across wide phylogenetic gaps, the accessory subunits of these complexes differ in identity and/or function when plants are compared with Saccharomyces cerevisiae (yeast), the model system for mitochondrial protein import. These differences include distinct protein import receptors in plants, different mechanistic operation of the intermembrane protein import system, the location and activity of peptidases, the function of inner-membrane translocases in linking the outer and inner membrane, and the association/regulation of mitochondrial protein import complexes with components of the respiratory chain. Additionally, plant mitochondria share proteins with plastids, i.e. dual-targeted proteins. Also, the developmental and cell-specific nature of mitochondrial biogenesis is an aspect not observed in single-celled systems that is readily apparent in studies in plants. This means that plants provide a valuable model system to study the various regulatory processes associated with protein import and mitochondrial biogenesis.
Original languageEnglish
Pages (from-to)6301-6335
JournalJournal of Experimental Botany
Volume65
Issue number22
Early online date16 Oct 2014
DOIs
Publication statusPublished - Dec 2014

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Mitochondrial Proteins
imports
Mitochondria
mitochondria
Proteins
proteins
Organelle Biogenesis
Chloroplast Proteins
Protein Precursors
Membranes
Protein Subunits
Electron Transport
Saccharomyces cerevisiae
Peptide Hydrolases
Yeasts
peptidases
electron transport chain
protein subunits
sorting
plastids

Cite this

Murcha, M., Kmiec, B., Kubiszewski-Jakubiak, S., Teixeira, P. F., Glaser, E. G., & Whelan, J. M. (2014). Protein import into plant mitochondria: Signals, machinery, processing, and regulation. Journal of Experimental Botany, 65(22), 6301-6335. https://doi.org/10.1093/jxb/eru399
Murcha, Monika ; Kmiec, B. ; Kubiszewski-Jakubiak, S. ; Teixeira, P.F. ; Glaser, E.G. ; Whelan, J.M. / Protein import into plant mitochondria: Signals, machinery, processing, and regulation. In: Journal of Experimental Botany. 2014 ; Vol. 65, No. 22. pp. 6301-6335.
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Murcha, M, Kmiec, B, Kubiszewski-Jakubiak, S, Teixeira, PF, Glaser, EG & Whelan, JM 2014, 'Protein import into plant mitochondria: Signals, machinery, processing, and regulation' Journal of Experimental Botany, vol. 65, no. 22, pp. 6301-6335. https://doi.org/10.1093/jxb/eru399

Protein import into plant mitochondria: Signals, machinery, processing, and regulation. / Murcha, Monika; Kmiec, B.; Kubiszewski-Jakubiak, S.; Teixeira, P.F.; Glaser, E.G.; Whelan, J.M.

In: Journal of Experimental Botany, Vol. 65, No. 22, 12.2014, p. 6301-6335.

Research output: Contribution to journalReview article

TY - JOUR

T1 - Protein import into plant mitochondria: Signals, machinery, processing, and regulation

AU - Murcha, Monika

AU - Kmiec, B.

AU - Kubiszewski-Jakubiak, S.

AU - Teixeira, P.F.

AU - Glaser, E.G.

AU - Whelan, J.M.

PY - 2014/12

Y1 - 2014/12

N2 - © The Author 2014. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. The majority of more than 1000 proteins present in mitochondria are imported from nuclear-encoded, cytosolically synthesized precursor proteins. This impressive feat of transport and sorting is achieved by the combined action of targeting signals on mitochondrial proteins and the mitochondrial protein import apparatus. The mitochondrial protein import apparatus is composed of a number of multi-subunit protein complexes that recognize, translocate, and assemble mitochondrial proteins into functional complexes. While the core subunits involved in mitochondrial protein import are well conserved across wide phylogenetic gaps, the accessory subunits of these complexes differ in identity and/or function when plants are compared with Saccharomyces cerevisiae (yeast), the model system for mitochondrial protein import. These differences include distinct protein import receptors in plants, different mechanistic operation of the intermembrane protein import system, the location and activity of peptidases, the function of inner-membrane translocases in linking the outer and inner membrane, and the association/regulation of mitochondrial protein import complexes with components of the respiratory chain. Additionally, plant mitochondria share proteins with plastids, i.e. dual-targeted proteins. Also, the developmental and cell-specific nature of mitochondrial biogenesis is an aspect not observed in single-celled systems that is readily apparent in studies in plants. This means that plants provide a valuable model system to study the various regulatory processes associated with protein import and mitochondrial biogenesis.

AB - © The Author 2014. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. The majority of more than 1000 proteins present in mitochondria are imported from nuclear-encoded, cytosolically synthesized precursor proteins. This impressive feat of transport and sorting is achieved by the combined action of targeting signals on mitochondrial proteins and the mitochondrial protein import apparatus. The mitochondrial protein import apparatus is composed of a number of multi-subunit protein complexes that recognize, translocate, and assemble mitochondrial proteins into functional complexes. While the core subunits involved in mitochondrial protein import are well conserved across wide phylogenetic gaps, the accessory subunits of these complexes differ in identity and/or function when plants are compared with Saccharomyces cerevisiae (yeast), the model system for mitochondrial protein import. These differences include distinct protein import receptors in plants, different mechanistic operation of the intermembrane protein import system, the location and activity of peptidases, the function of inner-membrane translocases in linking the outer and inner membrane, and the association/regulation of mitochondrial protein import complexes with components of the respiratory chain. Additionally, plant mitochondria share proteins with plastids, i.e. dual-targeted proteins. Also, the developmental and cell-specific nature of mitochondrial biogenesis is an aspect not observed in single-celled systems that is readily apparent in studies in plants. This means that plants provide a valuable model system to study the various regulatory processes associated with protein import and mitochondrial biogenesis.

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DO - 10.1093/jxb/eru399

M3 - Review article

VL - 65

SP - 6301

EP - 6335

JO - Journal of Experimental Botany

JF - Journal of Experimental Botany

SN - 0022-0957

IS - 22

ER -