Protein Delivery into the Cell Cytosol using Non-Viral Nanocarriers

Yi-Wei Lee, David C. Luther, Jessica A. Kretzmann, Andrew Burden, Taewon Jeon, Shumei Zhai, Vincent M. Rotello

Research output: Contribution to journalReview article

2 Citations (Scopus)

Abstract

Protein delivery into cells is a potentially transformative tool for treating "undruggable" targets in diseases associated with protein deficiencies or mutations. The vast majority of these targets are accessed via the cytosol, a challenging prospect for proteins with therapeutic and diagnostic relevance. In this review we will present promising non-viral approaches for intracellular and ultimately cytosolic delivery of proteins using nanocarriers. We will also discuss the mechanistic properties that govern the efficacy of nanocarrier-mediated protein delivery, applications of nanomaterials, and key challenges and opportunities in the use of nanocarriers for intracellular protein delivery.

Original languageEnglish
Pages (from-to)3280-3292
Number of pages13
JournalTheranostics
Volume9
Issue number11
DOIs
Publication statusPublished - 2019

Cite this

Lee, Y-W., Luther, D. C., Kretzmann, J. A., Burden, A., Jeon, T., Zhai, S., & Rotello, V. M. (2019). Protein Delivery into the Cell Cytosol using Non-Viral Nanocarriers. Theranostics, 9(11), 3280-3292. https://doi.org/10.7150/thno.34412
Lee, Yi-Wei ; Luther, David C. ; Kretzmann, Jessica A. ; Burden, Andrew ; Jeon, Taewon ; Zhai, Shumei ; Rotello, Vincent M. / Protein Delivery into the Cell Cytosol using Non-Viral Nanocarriers. In: Theranostics. 2019 ; Vol. 9, No. 11. pp. 3280-3292.
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Lee, Y-W, Luther, DC, Kretzmann, JA, Burden, A, Jeon, T, Zhai, S & Rotello, VM 2019, 'Protein Delivery into the Cell Cytosol using Non-Viral Nanocarriers' Theranostics, vol. 9, no. 11, pp. 3280-3292. https://doi.org/10.7150/thno.34412

Protein Delivery into the Cell Cytosol using Non-Viral Nanocarriers. / Lee, Yi-Wei; Luther, David C.; Kretzmann, Jessica A.; Burden, Andrew; Jeon, Taewon; Zhai, Shumei; Rotello, Vincent M.

In: Theranostics, Vol. 9, No. 11, 2019, p. 3280-3292.

Research output: Contribution to journalReview article

TY - JOUR

T1 - Protein Delivery into the Cell Cytosol using Non-Viral Nanocarriers

AU - Lee, Yi-Wei

AU - Luther, David C.

AU - Kretzmann, Jessica A.

AU - Burden, Andrew

AU - Jeon, Taewon

AU - Zhai, Shumei

AU - Rotello, Vincent M.

PY - 2019

Y1 - 2019

N2 - Protein delivery into cells is a potentially transformative tool for treating "undruggable" targets in diseases associated with protein deficiencies or mutations. The vast majority of these targets are accessed via the cytosol, a challenging prospect for proteins with therapeutic and diagnostic relevance. In this review we will present promising non-viral approaches for intracellular and ultimately cytosolic delivery of proteins using nanocarriers. We will also discuss the mechanistic properties that govern the efficacy of nanocarrier-mediated protein delivery, applications of nanomaterials, and key challenges and opportunities in the use of nanocarriers for intracellular protein delivery.

AB - Protein delivery into cells is a potentially transformative tool for treating "undruggable" targets in diseases associated with protein deficiencies or mutations. The vast majority of these targets are accessed via the cytosol, a challenging prospect for proteins with therapeutic and diagnostic relevance. In this review we will present promising non-viral approaches for intracellular and ultimately cytosolic delivery of proteins using nanocarriers. We will also discuss the mechanistic properties that govern the efficacy of nanocarrier-mediated protein delivery, applications of nanomaterials, and key challenges and opportunities in the use of nanocarriers for intracellular protein delivery.

KW - intracellular protein delivery

KW - supramolecular assemblies

KW - protein therapeutics

KW - endosomal escape

KW - membrane fusion

KW - DRUG-DELIVERY

KW - INTRACELLULAR DELIVERY

KW - NANOPARTICLE DELIVERY

KW - GENE

KW - DNA

KW - FORMULATION

KW - MECHANISMS

KW - PEPTIDES

KW - POLYMERS

KW - MICELLES

U2 - 10.7150/thno.34412

DO - 10.7150/thno.34412

M3 - Review article

VL - 9

SP - 3280

EP - 3292

JO - Theranostics

JF - Theranostics

SN - 1838-7640

IS - 11

ER -

Lee Y-W, Luther DC, Kretzmann JA, Burden A, Jeon T, Zhai S et al. Protein Delivery into the Cell Cytosol using Non-Viral Nanocarriers. Theranostics. 2019;9(11):3280-3292. https://doi.org/10.7150/thno.34412