Abstract
Protein delivery into cells is a potentially transformative tool for treating "undruggable" targets in diseases associated with protein deficiencies or mutations. The vast majority of these targets are accessed via the cytosol, a challenging prospect for proteins with therapeutic and diagnostic relevance. In this review we will present promising non-viral approaches for intracellular and ultimately cytosolic delivery of proteins using nanocarriers. We will also discuss the mechanistic properties that govern the efficacy of nanocarrier-mediated protein delivery, applications of nanomaterials, and key challenges and opportunities in the use of nanocarriers for intracellular protein delivery.
| Original language | English |
|---|---|
| Pages (from-to) | 3280-3292 |
| Number of pages | 13 |
| Journal | Theranostics |
| Volume | 9 |
| Issue number | 11 |
| DOIs | |
| Publication status | Published - 2019 |
Cite this
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Protein Delivery into the Cell Cytosol using Non-Viral Nanocarriers. / Lee, Yi-Wei; Luther, David C.; Kretzmann, Jessica A.; Burden, Andrew; Jeon, Taewon; Zhai, Shumei; Rotello, Vincent M.
In: Theranostics, Vol. 9, No. 11, 2019, p. 3280-3292.Research output: Contribution to journal › Review article
TY - JOUR
T1 - Protein Delivery into the Cell Cytosol using Non-Viral Nanocarriers
AU - Lee, Yi-Wei
AU - Luther, David C.
AU - Kretzmann, Jessica A.
AU - Burden, Andrew
AU - Jeon, Taewon
AU - Zhai, Shumei
AU - Rotello, Vincent M.
PY - 2019
Y1 - 2019
N2 - Protein delivery into cells is a potentially transformative tool for treating "undruggable" targets in diseases associated with protein deficiencies or mutations. The vast majority of these targets are accessed via the cytosol, a challenging prospect for proteins with therapeutic and diagnostic relevance. In this review we will present promising non-viral approaches for intracellular and ultimately cytosolic delivery of proteins using nanocarriers. We will also discuss the mechanistic properties that govern the efficacy of nanocarrier-mediated protein delivery, applications of nanomaterials, and key challenges and opportunities in the use of nanocarriers for intracellular protein delivery.
AB - Protein delivery into cells is a potentially transformative tool for treating "undruggable" targets in diseases associated with protein deficiencies or mutations. The vast majority of these targets are accessed via the cytosol, a challenging prospect for proteins with therapeutic and diagnostic relevance. In this review we will present promising non-viral approaches for intracellular and ultimately cytosolic delivery of proteins using nanocarriers. We will also discuss the mechanistic properties that govern the efficacy of nanocarrier-mediated protein delivery, applications of nanomaterials, and key challenges and opportunities in the use of nanocarriers for intracellular protein delivery.
KW - intracellular protein delivery
KW - supramolecular assemblies
KW - protein therapeutics
KW - endosomal escape
KW - membrane fusion
KW - DRUG-DELIVERY
KW - INTRACELLULAR DELIVERY
KW - NANOPARTICLE DELIVERY
KW - GENE
KW - DNA
KW - FORMULATION
KW - MECHANISMS
KW - PEPTIDES
KW - POLYMERS
KW - MICELLES
U2 - 10.7150/thno.34412
DO - 10.7150/thno.34412
M3 - Review article
VL - 9
SP - 3280
EP - 3292
JO - Theranostics
JF - Theranostics
SN - 1838-7640
IS - 11
ER -