Prostaglandin E2 imprints a long-lasting effect on dendritic cell progenitors in the bone marrow

N.M. Scott, R.L. Ng, Shelley Gorman, M Norval, Jason Waithman, Prudence Hart

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Dendritic cells (DCs) that differentiate in vitro from the bone marrow (BM) of mice with prostaglandin E2 (PGE2)-associated inflammation of the skin, airways, or peritoneal cavity poorly initiate immune responses. To remove in vitro differentiation and allow BM-derived DCs to seed the periphery under steady-state conditions, as well as study the molecule proposed responsible, chimeric mice were engrafted for >16 wk with BM cells from mice exposed to PGE2. Serial PGE2-chimeric mice were established with BM cells from the primary chimeric mice. Immune responses in the airways and skin of the PGE2-chimeric mice and serial PGE2-chimeric mice were significantly attenuated. After inflammatory challenges by intranasal LPS, topical fluorescein isothiocyanate, and intraperitoneal alum, DCs, macrophages, and neutrophils trafficked poorly in PGE2-chimeric mice and serial PGE2-chimeric mice. Injection of BM-differentiated DCs from nonchimeric mice restored the reduced immune responses of PGE2-chimeric mice. DCs from BM of 16-wk-engrafted PGE2-chimeric and serial PGE2-chimeric mice resembled cells differentiated from BM exposed to PGE2 for only 3 d, demonstrating the long-lasting effect of PGE2 on DC progenitors. PGE2 attenuates systemic immune responses by modulating myeloid cell progenitors in the BM such that BM-derived, terminally differentiated myeloid cells have poor trafficking ability to sites of need.
Original languageEnglish
Pages (from-to)225-232
JournalJournal of Leukocyte Biology
Volume95
Issue number2
Early online date27 Aug 2013
DOIs
Publication statusPublished - Feb 2014

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Dinoprostone
Dendritic Cells
Bone Marrow
Bone Marrow Cells
Myeloid Progenitor Cells
Skin
Peritoneal Cavity
Myeloid Cells
Fluorescein
Seeds
Neutrophils
Macrophages
Inflammation

Cite this

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title = "Prostaglandin E2 imprints a long-lasting effect on dendritic cell progenitors in the bone marrow",
abstract = "Dendritic cells (DCs) that differentiate in vitro from the bone marrow (BM) of mice with prostaglandin E2 (PGE2)-associated inflammation of the skin, airways, or peritoneal cavity poorly initiate immune responses. To remove in vitro differentiation and allow BM-derived DCs to seed the periphery under steady-state conditions, as well as study the molecule proposed responsible, chimeric mice were engrafted for >16 wk with BM cells from mice exposed to PGE2. Serial PGE2-chimeric mice were established with BM cells from the primary chimeric mice. Immune responses in the airways and skin of the PGE2-chimeric mice and serial PGE2-chimeric mice were significantly attenuated. After inflammatory challenges by intranasal LPS, topical fluorescein isothiocyanate, and intraperitoneal alum, DCs, macrophages, and neutrophils trafficked poorly in PGE2-chimeric mice and serial PGE2-chimeric mice. Injection of BM-differentiated DCs from nonchimeric mice restored the reduced immune responses of PGE2-chimeric mice. DCs from BM of 16-wk-engrafted PGE2-chimeric and serial PGE2-chimeric mice resembled cells differentiated from BM exposed to PGE2 for only 3 d, demonstrating the long-lasting effect of PGE2 on DC progenitors. PGE2 attenuates systemic immune responses by modulating myeloid cell progenitors in the BM such that BM-derived, terminally differentiated myeloid cells have poor trafficking ability to sites of need.",
author = "N.M. Scott and R.L. Ng and Shelley Gorman and M Norval and Jason Waithman and Prudence Hart",
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Prostaglandin E2 imprints a long-lasting effect on dendritic cell progenitors in the bone marrow. / Scott, N.M.; Ng, R.L.; Gorman, Shelley; Norval, M; Waithman, Jason; Hart, Prudence.

In: Journal of Leukocyte Biology, Vol. 95, No. 2, 02.2014, p. 225-232.

Research output: Contribution to journalArticle

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AU - Ng, R.L.

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AU - Hart, Prudence

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