TY - JOUR
T1 - Prominent IgE-binding and cytokine-inducing capacities of a newly cloned N-terminal region of der f 14, an apolipophorin-like house dust mite allergen
AU - Elramlawy, Kareem Gamal
AU - Fujimura, Takashi
AU - Aki, Tsunehiro
AU - Okada, Akiyasu
AU - Suzuki, Takayuki
AU - Abe, Takuya
AU - Hayashi, Takaharu
AU - Epton, Michael J.
AU - Thomas, Wayne R.
AU - Rafeet, Inas Hussein
AU - Al-Azhary, Diaa Beshr
AU - Ono, Kazuhisa
AU - Kawamoto, Seiji
PY - 2018/1/1
Y1 - 2018/1/1
N2 - We previously characterized a 177-κDa allergen, M-177, from Dermatophagoides farinae. Thereafter, a counterpart to M-177 for Euroglyphus maynei was cloned as Eur m 14, and its sequence revealed that two environmental allergens, Mag 1 and Mag 3, are digested fragments of M-177. The aims of this study were to clone the cDNA of Der f 14 corresponding to M-177 and to elucidate the allergenic capacities of the N-terminal fragment of Der f 14 (Der f 14-N). Recombinant allergens were produced as triggerfactor-fused proteins in Escherichia coli. Der f 14-N showed the highest IgE-binding frequency among Der f 14-derived fragments in patients allergic to house dust mite by enzyme-linked immunosorbent assay. Der f 14-N showed the highest capacity to induce cell proliferation in murine lymphocyte and human peripheral mononuclear cells among Der f 14-derived fragments. Der f 14-N induced IL-13, IFN-γ and IL-17 production more than Der f 1 and Der f 2 in mouse, and induced IL-5 and IFN-γ production at levels comparable to those of Der f 1 and Der f 2 in some patients. The high prevalence of IgE binding to the Der f 14-N indicates that it could be an important mite allergen.
AB - We previously characterized a 177-κDa allergen, M-177, from Dermatophagoides farinae. Thereafter, a counterpart to M-177 for Euroglyphus maynei was cloned as Eur m 14, and its sequence revealed that two environmental allergens, Mag 1 and Mag 3, are digested fragments of M-177. The aims of this study were to clone the cDNA of Der f 14 corresponding to M-177 and to elucidate the allergenic capacities of the N-terminal fragment of Der f 14 (Der f 14-N). Recombinant allergens were produced as triggerfactor-fused proteins in Escherichia coli. Der f 14-N showed the highest IgE-binding frequency among Der f 14-derived fragments in patients allergic to house dust mite by enzyme-linked immunosorbent assay. Der f 14-N showed the highest capacity to induce cell proliferation in murine lymphocyte and human peripheral mononuclear cells among Der f 14-derived fragments. Der f 14-N induced IL-13, IFN-γ and IL-17 production more than Der f 1 and Der f 2 in mouse, and induced IL-5 and IFN-γ production at levels comparable to those of Der f 1 and Der f 2 in some patients. The high prevalence of IgE binding to the Der f 14-N indicates that it could be an important mite allergen.
KW - apolipophorin
KW - Der f 14
KW - Dermatophagoides farinae
KW - environmental allergen
KW - house dust mite
UR - http://www.scopus.com/inward/record.url?scp=85040700854&partnerID=8YFLogxK
U2 - 10.1093/jb/mvx060
DO - 10.1093/jb/mvx060
M3 - Article
C2 - 28992042
AN - SCOPUS:85040700854
SN - 0021-924X
VL - 163
SP - 51
EP - 60
JO - Journal of Biochemistry
JF - Journal of Biochemistry
IS - 1
ER -