Prominent IgE-binding and cytokine-inducing capacities of a newly cloned N-terminal region of der f 14, an apolipophorin-like house dust mite allergen

Kareem Gamal Elramlawy, Takashi Fujimura, Tsunehiro Aki, Akiyasu Okada, Takayuki Suzuki, Takuya Abe, Takaharu Hayashi, Michael J. Epton, Wayne R. Thomas, Inas Hussein Rafeet, Diaa Beshr Al-Azhary, Kazuhisa Ono, Seiji Kawamoto

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We previously characterized a 177-κDa allergen, M-177, from Dermatophagoides farinae. Thereafter, a counterpart to M-177 for Euroglyphus maynei was cloned as Eur m 14, and its sequence revealed that two environmental allergens, Mag 1 and Mag 3, are digested fragments of M-177. The aims of this study were to clone the cDNA of Der f 14 corresponding to M-177 and to elucidate the allergenic capacities of the N-terminal fragment of Der f 14 (Der f 14-N). Recombinant allergens were produced as triggerfactor-fused proteins in Escherichia coli. Der f 14-N showed the highest IgE-binding frequency among Der f 14-derived fragments in patients allergic to house dust mite by enzyme-linked immunosorbent assay. Der f 14-N showed the highest capacity to induce cell proliferation in murine lymphocyte and human peripheral mononuclear cells among Der f 14-derived fragments. Der f 14-N induced IL-13, IFN-γ and IL-17 production more than Der f 1 and Der f 2 in mouse, and induced IL-5 and IFN-γ production at levels comparable to those of Der f 1 and Der f 2 in some patients. The high prevalence of IgE binding to the Der f 14-N indicates that it could be an important mite allergen.

Original languageEnglish
Pages (from-to)51-60
Number of pages10
JournalJournal of Biochemistry
Volume163
Issue number1
DOIs
Publication statusPublished - 1 Jan 2018

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Dermatophagoides Antigens
Allergens
Immunoglobulin E
Cytokines
Pyroglyphidae
Immunosorbents
Interleukin-13
Lymphocytes
Interleukin-17
Interleukin-5
Cell proliferation
Dermatophagoides farinae
Escherichia coli
Dust
Mites
Escherichia coli Proteins
Assays
Complementary DNA
Clone Cells
Enzyme-Linked Immunosorbent Assay

Cite this

Elramlawy, Kareem Gamal ; Fujimura, Takashi ; Aki, Tsunehiro ; Okada, Akiyasu ; Suzuki, Takayuki ; Abe, Takuya ; Hayashi, Takaharu ; Epton, Michael J. ; Thomas, Wayne R. ; Rafeet, Inas Hussein ; Al-Azhary, Diaa Beshr ; Ono, Kazuhisa ; Kawamoto, Seiji. / Prominent IgE-binding and cytokine-inducing capacities of a newly cloned N-terminal region of der f 14, an apolipophorin-like house dust mite allergen. In: Journal of Biochemistry. 2018 ; Vol. 163, No. 1. pp. 51-60.
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abstract = "We previously characterized a 177-κDa allergen, M-177, from Dermatophagoides farinae. Thereafter, a counterpart to M-177 for Euroglyphus maynei was cloned as Eur m 14, and its sequence revealed that two environmental allergens, Mag 1 and Mag 3, are digested fragments of M-177. The aims of this study were to clone the cDNA of Der f 14 corresponding to M-177 and to elucidate the allergenic capacities of the N-terminal fragment of Der f 14 (Der f 14-N). Recombinant allergens were produced as triggerfactor-fused proteins in Escherichia coli. Der f 14-N showed the highest IgE-binding frequency among Der f 14-derived fragments in patients allergic to house dust mite by enzyme-linked immunosorbent assay. Der f 14-N showed the highest capacity to induce cell proliferation in murine lymphocyte and human peripheral mononuclear cells among Der f 14-derived fragments. Der f 14-N induced IL-13, IFN-γ and IL-17 production more than Der f 1 and Der f 2 in mouse, and induced IL-5 and IFN-γ production at levels comparable to those of Der f 1 and Der f 2 in some patients. The high prevalence of IgE binding to the Der f 14-N indicates that it could be an important mite allergen.",
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author = "Elramlawy, {Kareem Gamal} and Takashi Fujimura and Tsunehiro Aki and Akiyasu Okada and Takayuki Suzuki and Takuya Abe and Takaharu Hayashi and Epton, {Michael J.} and Thomas, {Wayne R.} and Rafeet, {Inas Hussein} and Al-Azhary, {Diaa Beshr} and Kazuhisa Ono and Seiji Kawamoto",
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Elramlawy, KG, Fujimura, T, Aki, T, Okada, A, Suzuki, T, Abe, T, Hayashi, T, Epton, MJ, Thomas, WR, Rafeet, IH, Al-Azhary, DB, Ono, K & Kawamoto, S 2018, 'Prominent IgE-binding and cytokine-inducing capacities of a newly cloned N-terminal region of der f 14, an apolipophorin-like house dust mite allergen' Journal of Biochemistry, vol. 163, no. 1, pp. 51-60. https://doi.org/10.1093/jb/mvx060

Prominent IgE-binding and cytokine-inducing capacities of a newly cloned N-terminal region of der f 14, an apolipophorin-like house dust mite allergen. / Elramlawy, Kareem Gamal; Fujimura, Takashi; Aki, Tsunehiro; Okada, Akiyasu; Suzuki, Takayuki; Abe, Takuya; Hayashi, Takaharu; Epton, Michael J.; Thomas, Wayne R.; Rafeet, Inas Hussein; Al-Azhary, Diaa Beshr; Ono, Kazuhisa; Kawamoto, Seiji.

In: Journal of Biochemistry, Vol. 163, No. 1, 01.01.2018, p. 51-60.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Prominent IgE-binding and cytokine-inducing capacities of a newly cloned N-terminal region of der f 14, an apolipophorin-like house dust mite allergen

AU - Elramlawy, Kareem Gamal

AU - Fujimura, Takashi

AU - Aki, Tsunehiro

AU - Okada, Akiyasu

AU - Suzuki, Takayuki

AU - Abe, Takuya

AU - Hayashi, Takaharu

AU - Epton, Michael J.

AU - Thomas, Wayne R.

AU - Rafeet, Inas Hussein

AU - Al-Azhary, Diaa Beshr

AU - Ono, Kazuhisa

AU - Kawamoto, Seiji

PY - 2018/1/1

Y1 - 2018/1/1

N2 - We previously characterized a 177-κDa allergen, M-177, from Dermatophagoides farinae. Thereafter, a counterpart to M-177 for Euroglyphus maynei was cloned as Eur m 14, and its sequence revealed that two environmental allergens, Mag 1 and Mag 3, are digested fragments of M-177. The aims of this study were to clone the cDNA of Der f 14 corresponding to M-177 and to elucidate the allergenic capacities of the N-terminal fragment of Der f 14 (Der f 14-N). Recombinant allergens were produced as triggerfactor-fused proteins in Escherichia coli. Der f 14-N showed the highest IgE-binding frequency among Der f 14-derived fragments in patients allergic to house dust mite by enzyme-linked immunosorbent assay. Der f 14-N showed the highest capacity to induce cell proliferation in murine lymphocyte and human peripheral mononuclear cells among Der f 14-derived fragments. Der f 14-N induced IL-13, IFN-γ and IL-17 production more than Der f 1 and Der f 2 in mouse, and induced IL-5 and IFN-γ production at levels comparable to those of Der f 1 and Der f 2 in some patients. The high prevalence of IgE binding to the Der f 14-N indicates that it could be an important mite allergen.

AB - We previously characterized a 177-κDa allergen, M-177, from Dermatophagoides farinae. Thereafter, a counterpart to M-177 for Euroglyphus maynei was cloned as Eur m 14, and its sequence revealed that two environmental allergens, Mag 1 and Mag 3, are digested fragments of M-177. The aims of this study were to clone the cDNA of Der f 14 corresponding to M-177 and to elucidate the allergenic capacities of the N-terminal fragment of Der f 14 (Der f 14-N). Recombinant allergens were produced as triggerfactor-fused proteins in Escherichia coli. Der f 14-N showed the highest IgE-binding frequency among Der f 14-derived fragments in patients allergic to house dust mite by enzyme-linked immunosorbent assay. Der f 14-N showed the highest capacity to induce cell proliferation in murine lymphocyte and human peripheral mononuclear cells among Der f 14-derived fragments. Der f 14-N induced IL-13, IFN-γ and IL-17 production more than Der f 1 and Der f 2 in mouse, and induced IL-5 and IFN-γ production at levels comparable to those of Der f 1 and Der f 2 in some patients. The high prevalence of IgE binding to the Der f 14-N indicates that it could be an important mite allergen.

KW - apolipophorin

KW - Der f 14

KW - Dermatophagoides farinae

KW - environmental allergen

KW - house dust mite

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U2 - 10.1093/jb/mvx060

DO - 10.1093/jb/mvx060

M3 - Article

VL - 163

SP - 51

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JO - Journal of Biochemistry

JF - Journal of Biochemistry

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