Prominent IgE-binding and cytokine-inducing capacities of a newly cloned N-terminal region of der f 14, an apolipophorin-like house dust mite allergen

Kareem Gamal Elramlawy, Takashi Fujimura, Tsunehiro Aki, Akiyasu Okada, Takayuki Suzuki, Takuya Abe, Takaharu Hayashi, Michael J. Epton, Wayne R. Thomas, Inas Hussein Rafeet, Diaa Beshr Al-Azhary, Kazuhisa Ono, Seiji Kawamoto

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We previously characterized a 177-κDa allergen, M-177, from Dermatophagoides farinae. Thereafter, a counterpart to M-177 for Euroglyphus maynei was cloned as Eur m 14, and its sequence revealed that two environmental allergens, Mag 1 and Mag 3, are digested fragments of M-177. The aims of this study were to clone the cDNA of Der f 14 corresponding to M-177 and to elucidate the allergenic capacities of the N-terminal fragment of Der f 14 (Der f 14-N). Recombinant allergens were produced as triggerfactor-fused proteins in Escherichia coli. Der f 14-N showed the highest IgE-binding frequency among Der f 14-derived fragments in patients allergic to house dust mite by enzyme-linked immunosorbent assay. Der f 14-N showed the highest capacity to induce cell proliferation in murine lymphocyte and human peripheral mononuclear cells among Der f 14-derived fragments. Der f 14-N induced IL-13, IFN-γ and IL-17 production more than Der f 1 and Der f 2 in mouse, and induced IL-5 and IFN-γ production at levels comparable to those of Der f 1 and Der f 2 in some patients. The high prevalence of IgE binding to the Der f 14-N indicates that it could be an important mite allergen.

Original languageEnglish
Pages (from-to)51-60
Number of pages10
JournalJournal of Biochemistry
Volume163
Issue number1
DOIs
Publication statusPublished - 1 Jan 2018

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