Profiling and genetic control of the murine immunoglobulin G glycome

J. Krištić, O.O. Zaytseva, R. Ram, Q. Nguyen, M. Novokmet, F. Vučković, M. Vilaj, I. Trbojević-Akmačić, M. Pezer, K.M. Davern, G. Morahan, G. Lauc

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Abstract

Immunoglobulin G (IgG) glycosylation is essential for function of the immune system, but the genetic and environmental factors that underlie its inter-individual variability are not well defined. The Collaborative Cross (CC) genetic resource harnesses over 90% of the common genetic variation of the mouse. By analyzing the IgG glycome composition of 95 CC strains, we made several important observations: (i) glycome variation between mouse strains was higher than between individual humans, despite all mice having the same environmental influences; (ii) five genetic loci were found to be associated with murine IgG glycosylation; (iii) variants outside traditional glycosylation site motifs affected glycome variation; (iv) bisecting N-acetylglucosamine (GlcNAc) was produced by several strains although most previous studies have reported the absence of glycans containing the bisecting GlcNAc on murine IgGs; and (v) common laboratory mouse strains are not optimal animal models for studying effects of glycosylation on IgG function.

Original languageEnglish
Pages (from-to)516-524
Number of pages9
JournalNature Chemical Biology
Volume14
Issue number5
DOIs
Publication statusPublished - 1 May 2018

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Glycosylation
Immunoglobulin G
Genetic Crosses
Genetic Loci
Acetylglucosamine
Polysaccharides
Immune System
Animal Models

Cite this

Krištić, J., Zaytseva, O. O., Ram, R., Nguyen, Q., Novokmet, M., Vučković, F., ... Lauc, G. (2018). Profiling and genetic control of the murine immunoglobulin G glycome. Nature Chemical Biology, 14(5), 516-524. https://doi.org/10.1038/s41589-018-0034-3
Krištić, J. ; Zaytseva, O.O. ; Ram, R. ; Nguyen, Q. ; Novokmet, M. ; Vučković, F. ; Vilaj, M. ; Trbojević-Akmačić, I. ; Pezer, M. ; Davern, K.M. ; Morahan, G. ; Lauc, G. / Profiling and genetic control of the murine immunoglobulin G glycome. In: Nature Chemical Biology. 2018 ; Vol. 14, No. 5. pp. 516-524.
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Krištić, J, Zaytseva, OO, Ram, R, Nguyen, Q, Novokmet, M, Vučković, F, Vilaj, M, Trbojević-Akmačić, I, Pezer, M, Davern, KM, Morahan, G & Lauc, G 2018, 'Profiling and genetic control of the murine immunoglobulin G glycome' Nature Chemical Biology, vol. 14, no. 5, pp. 516-524. https://doi.org/10.1038/s41589-018-0034-3

Profiling and genetic control of the murine immunoglobulin G glycome. / Krištić, J.; Zaytseva, O.O.; Ram, R.; Nguyen, Q.; Novokmet, M.; Vučković, F.; Vilaj, M.; Trbojević-Akmačić, I.; Pezer, M.; Davern, K.M.; Morahan, G.; Lauc, G.

In: Nature Chemical Biology, Vol. 14, No. 5, 01.05.2018, p. 516-524.

Research output: Contribution to journalArticle

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Krištić J, Zaytseva OO, Ram R, Nguyen Q, Novokmet M, Vučković F et al. Profiling and genetic control of the murine immunoglobulin G glycome. Nature Chemical Biology. 2018 May 1;14(5):516-524. https://doi.org/10.1038/s41589-018-0034-3