Abstract
Immunoglobulin G (IgG) glycosylation is essential for function of the immune system, but the genetic and environmental factors that underlie its inter-individual variability are not well defined. The Collaborative Cross (CC) genetic resource harnesses over 90% of the common genetic variation of the mouse. By analyzing the IgG glycome composition of 95 CC strains, we made several important observations: (i) glycome variation between mouse strains was higher than between individual humans, despite all mice having the same environmental influences; (ii) five genetic loci were found to be associated with murine IgG glycosylation; (iii) variants outside traditional glycosylation site motifs affected glycome variation; (iv) bisecting N-acetylglucosamine (GlcNAc) was produced by several strains although most previous studies have reported the absence of glycans containing the bisecting GlcNAc on murine IgGs; and (v) common laboratory mouse strains are not optimal animal models for studying effects of glycosylation on IgG function.
| Original language | English |
|---|---|
| Pages (from-to) | 516-524 |
| Number of pages | 9 |
| Journal | Nature Chemical Biology |
| Volume | 14 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 1 May 2018 |
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Profiling and genetic control of the murine immunoglobulin G glycome. / Krištić, J.; Zaytseva, O.O.; Ram, R.; Nguyen, Q.; Novokmet, M.; Vučković, F.; Vilaj, M.; Trbojević-Akmačić, I.; Pezer, M.; Davern, K.M.; Morahan, G.; Lauc, G.
In: Nature Chemical Biology, Vol. 14, No. 5, 01.05.2018, p. 516-524.Research output: Contribution to journal › Article
TY - JOUR
T1 - Profiling and genetic control of the murine immunoglobulin G glycome
AU - Krištić, J.
AU - Zaytseva, O.O.
AU - Ram, R.
AU - Nguyen, Q.
AU - Novokmet, M.
AU - Vučković, F.
AU - Vilaj, M.
AU - Trbojević-Akmačić, I.
AU - Pezer, M.
AU - Davern, K.M.
AU - Morahan, G.
AU - Lauc, G.
PY - 2018/5/1
Y1 - 2018/5/1
N2 - Immunoglobulin G (IgG) glycosylation is essential for function of the immune system, but the genetic and environmental factors that underlie its inter-individual variability are not well defined. The Collaborative Cross (CC) genetic resource harnesses over 90% of the common genetic variation of the mouse. By analyzing the IgG glycome composition of 95 CC strains, we made several important observations: (i) glycome variation between mouse strains was higher than between individual humans, despite all mice having the same environmental influences; (ii) five genetic loci were found to be associated with murine IgG glycosylation; (iii) variants outside traditional glycosylation site motifs affected glycome variation; (iv) bisecting N-acetylglucosamine (GlcNAc) was produced by several strains although most previous studies have reported the absence of glycans containing the bisecting GlcNAc on murine IgGs; and (v) common laboratory mouse strains are not optimal animal models for studying effects of glycosylation on IgG function.
AB - Immunoglobulin G (IgG) glycosylation is essential for function of the immune system, but the genetic and environmental factors that underlie its inter-individual variability are not well defined. The Collaborative Cross (CC) genetic resource harnesses over 90% of the common genetic variation of the mouse. By analyzing the IgG glycome composition of 95 CC strains, we made several important observations: (i) glycome variation between mouse strains was higher than between individual humans, despite all mice having the same environmental influences; (ii) five genetic loci were found to be associated with murine IgG glycosylation; (iii) variants outside traditional glycosylation site motifs affected glycome variation; (iv) bisecting N-acetylglucosamine (GlcNAc) was produced by several strains although most previous studies have reported the absence of glycans containing the bisecting GlcNAc on murine IgGs; and (v) common laboratory mouse strains are not optimal animal models for studying effects of glycosylation on IgG function.
UR - http://www.scopus.com/inward/record.url?scp=85045108255&partnerID=8YFLogxK
U2 - 10.1038/s41589-018-0034-3
DO - 10.1038/s41589-018-0034-3
M3 - Article
VL - 14
SP - 516
EP - 524
JO - Nature Chemical Biology
JF - Nature Chemical Biology
SN - 1552-4450
IS - 5
ER -