Procyanidin A2 in the Australian plant Alectryon oleifolius has anthelmintic activity against equine cyathostomins in vitro

S. E. Payne, G. R. Flematti, A. Reeder, A. C. Kotze, Z. Durmic, P. E. Vercoe

Research output: Contribution to journalArticle

Abstract

There is a need to investigate new methods of controlling cyathostomins in horses due to increasing anthelmintic resistance amongst these parasites. In a previous study we identified the Australian plant Alectryon oleifolius as having anthelmintic activity towards cyathostomins. This study aimed to isolate and identify the bioactive compound(s) responsible for all or part of this anthelmintic activity and quantify its activity in vitro. The condensed tannin procyanidin A2 was isolated from the plant through a process of bioassay guided fractionation and identified using 1D and 2D nuclear magnetic resonance spectroscopy and high performance liquid chromatography with mass spectrometry. Procyanidin A2 demonstrated significant anthelmintic activity in larval development assays, completely inhibiting development from egg to third larval stage at concentrations as low as 50 μg/mL and having an IC50 value of 12.6 μg/mL. Procyanidin A2 also significantly inhibited larval migration at concentrations of 25 μg/mL. This study indicates that procyanidin A2 is the principal anthelmintic compound in extracts from A. oleifolius, and further highlights the potential for the use of this plant as a component of cyathostomin control programs in the future.

LanguageEnglish
Pages63-69
Number of pages7
JournalVeterinary Parasitology
Volume249
DOIs
StatePublished - 15 Jan 2018

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Cyathostominae
Anthelmintics
anthelmintics
Horses
horses
Proanthocyanidins
proanthocyanidins
Biological Assay
larval development
Ovum
nuclear magnetic resonance spectroscopy
Mass Spectrometry
fractionation
Parasites
Magnetic Resonance Spectroscopy
high performance liquid chromatography
bioassays
High Pressure Liquid Chromatography
procyanidin A2
In Vitro Techniques

Cite this

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abstract = "There is a need to investigate new methods of controlling cyathostomins in horses due to increasing anthelmintic resistance amongst these parasites. In a previous study we identified the Australian plant Alectryon oleifolius as having anthelmintic activity towards cyathostomins. This study aimed to isolate and identify the bioactive compound(s) responsible for all or part of this anthelmintic activity and quantify its activity in vitro. The condensed tannin procyanidin A2 was isolated from the plant through a process of bioassay guided fractionation and identified using 1D and 2D nuclear magnetic resonance spectroscopy and high performance liquid chromatography with mass spectrometry. Procyanidin A2 demonstrated significant anthelmintic activity in larval development assays, completely inhibiting development from egg to third larval stage at concentrations as low as 50 μg/mL and having an IC50 value of 12.6 μg/mL. Procyanidin A2 also significantly inhibited larval migration at concentrations of 25 μg/mL. This study indicates that procyanidin A2 is the principal anthelmintic compound in extracts from A. oleifolius, and further highlights the potential for the use of this plant as a component of cyathostomin control programs in the future.",
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Procyanidin A2 in the Australian plant Alectryon oleifolius has anthelmintic activity against equine cyathostomins in vitro. / Payne, S. E.; Flematti, G. R.; Reeder, A.; Kotze, A. C.; Durmic, Z.; Vercoe, P. E.

In: Veterinary Parasitology, Vol. 249, 15.01.2018, p. 63-69.

Research output: Contribution to journalArticle

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AU - Vercoe,P. E.

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