Prevalence and antibiotic susceptibility patterns of enteric bacterial pathogens in human and non-human sources in an urban informal settlement in Cape Town, South Africa

John Bosco Kalule, Anthony M Smith, Mjikisile Vulindhlu, Nomsa P Tau, Mark P Nicol, Karen H Keddy, Lourens Robberts

Research output: Contribution to journalArticle

Abstract

BACKGROUND: In light of rampant childhood diarrhoea, this study investigated bacterial pathogens from human and non-human sources in an urban informal settlement. Meat from informal abattoirs (n = 85), river water (n = 64), and diarrheic stool (n = 66) were collected between September 2015 and May 2016. A duplex real-time PCR, gel-based PCR, and CHROMagar™STEC were used to screen Tryptic Soy Broth (TSB) for diarrheic E. coli. Standard methods were used to screen for other selected food and waterborne bacterial pathogens.

RESULTS: Pathogens isolated from stool, meat, and surface water included Salmonella enterica (6, 5, 0%), Plesiomonas shigelloides (9, 0, 17%), Aeromonas sobria (3, 3, 0%), Campylobacter jejuni (5, 5, 0%), Shigella flexneri (17, 5, 0%), Vibrio vulnificus (0, 0, 9%), and diarrheic E. coli (21, 3, 7%) respectively. All the isolates were resistant to trimethoprim-sulphamethoxazole.

CONCLUSIONS: There was a high burden of drug resistant diarrheal pathogens in the stool, surface water and meat from informal slaughter. Integrated control measures are needed to ensure food safety and to prevent the spread of drug resistant pathogens in similar settings.

Original languageEnglish
Article number244
JournalBMC Microbiology
Volume19
Issue number1
DOIs
Publication statusPublished - 6 Nov 2019
Externally publishedYes

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South Africa
Meat
Anti-Bacterial Agents
Water
Plesiomonas
Vibrio vulnificus
Escherichia coli
Shigella flexneri
Aeromonas
Sulfamethoxazole
Campylobacter jejuni
Trimethoprim
Abattoirs
Salmonella enterica
Food Safety
Rivers
Pharmaceutical Preparations
Real-Time Polymerase Chain Reaction
Diarrhea
Gels

Cite this

Kalule, John Bosco ; Smith, Anthony M ; Vulindhlu, Mjikisile ; Tau, Nomsa P ; Nicol, Mark P ; Keddy, Karen H ; Robberts, Lourens. / Prevalence and antibiotic susceptibility patterns of enteric bacterial pathogens in human and non-human sources in an urban informal settlement in Cape Town, South Africa. In: BMC Microbiology. 2019 ; Vol. 19, No. 1.
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abstract = "BACKGROUND: In light of rampant childhood diarrhoea, this study investigated bacterial pathogens from human and non-human sources in an urban informal settlement. Meat from informal abattoirs (n = 85), river water (n = 64), and diarrheic stool (n = 66) were collected between September 2015 and May 2016. A duplex real-time PCR, gel-based PCR, and CHROMagar™STEC were used to screen Tryptic Soy Broth (TSB) for diarrheic E. coli. Standard methods were used to screen for other selected food and waterborne bacterial pathogens.RESULTS: Pathogens isolated from stool, meat, and surface water included Salmonella enterica (6, 5, 0{\%}), Plesiomonas shigelloides (9, 0, 17{\%}), Aeromonas sobria (3, 3, 0{\%}), Campylobacter jejuni (5, 5, 0{\%}), Shigella flexneri (17, 5, 0{\%}), Vibrio vulnificus (0, 0, 9{\%}), and diarrheic E. coli (21, 3, 7{\%}) respectively. All the isolates were resistant to trimethoprim-sulphamethoxazole.CONCLUSIONS: There was a high burden of drug resistant diarrheal pathogens in the stool, surface water and meat from informal slaughter. Integrated control measures are needed to ensure food safety and to prevent the spread of drug resistant pathogens in similar settings.",
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Prevalence and antibiotic susceptibility patterns of enteric bacterial pathogens in human and non-human sources in an urban informal settlement in Cape Town, South Africa. / Kalule, John Bosco; Smith, Anthony M; Vulindhlu, Mjikisile; Tau, Nomsa P; Nicol, Mark P; Keddy, Karen H; Robberts, Lourens.

In: BMC Microbiology, Vol. 19, No. 1, 244, 06.11.2019.

Research output: Contribution to journalArticle

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T1 - Prevalence and antibiotic susceptibility patterns of enteric bacterial pathogens in human and non-human sources in an urban informal settlement in Cape Town, South Africa

AU - Kalule, John Bosco

AU - Smith, Anthony M

AU - Vulindhlu, Mjikisile

AU - Tau, Nomsa P

AU - Nicol, Mark P

AU - Keddy, Karen H

AU - Robberts, Lourens

PY - 2019/11/6

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N2 - BACKGROUND: In light of rampant childhood diarrhoea, this study investigated bacterial pathogens from human and non-human sources in an urban informal settlement. Meat from informal abattoirs (n = 85), river water (n = 64), and diarrheic stool (n = 66) were collected between September 2015 and May 2016. A duplex real-time PCR, gel-based PCR, and CHROMagar™STEC were used to screen Tryptic Soy Broth (TSB) for diarrheic E. coli. Standard methods were used to screen for other selected food and waterborne bacterial pathogens.RESULTS: Pathogens isolated from stool, meat, and surface water included Salmonella enterica (6, 5, 0%), Plesiomonas shigelloides (9, 0, 17%), Aeromonas sobria (3, 3, 0%), Campylobacter jejuni (5, 5, 0%), Shigella flexneri (17, 5, 0%), Vibrio vulnificus (0, 0, 9%), and diarrheic E. coli (21, 3, 7%) respectively. All the isolates were resistant to trimethoprim-sulphamethoxazole.CONCLUSIONS: There was a high burden of drug resistant diarrheal pathogens in the stool, surface water and meat from informal slaughter. Integrated control measures are needed to ensure food safety and to prevent the spread of drug resistant pathogens in similar settings.

AB - BACKGROUND: In light of rampant childhood diarrhoea, this study investigated bacterial pathogens from human and non-human sources in an urban informal settlement. Meat from informal abattoirs (n = 85), river water (n = 64), and diarrheic stool (n = 66) were collected between September 2015 and May 2016. A duplex real-time PCR, gel-based PCR, and CHROMagar™STEC were used to screen Tryptic Soy Broth (TSB) for diarrheic E. coli. Standard methods were used to screen for other selected food and waterborne bacterial pathogens.RESULTS: Pathogens isolated from stool, meat, and surface water included Salmonella enterica (6, 5, 0%), Plesiomonas shigelloides (9, 0, 17%), Aeromonas sobria (3, 3, 0%), Campylobacter jejuni (5, 5, 0%), Shigella flexneri (17, 5, 0%), Vibrio vulnificus (0, 0, 9%), and diarrheic E. coli (21, 3, 7%) respectively. All the isolates were resistant to trimethoprim-sulphamethoxazole.CONCLUSIONS: There was a high burden of drug resistant diarrheal pathogens in the stool, surface water and meat from informal slaughter. Integrated control measures are needed to ensure food safety and to prevent the spread of drug resistant pathogens in similar settings.

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