Presence of commensal house dust mite allergen in human gastrointestinal tract: A potential contributor to intestinal barrier dysfunction

Meri K. Tulic, Mylene Vivinus-Nébot, Akila Rekima, Samara Rabelo Medeiros, Chrystelle Bonnart, Haining Shi, Allan Walker, Raffaella Dainese, Julien Boyer, Nathalie Vergnolle, Thierry Piche, Valérie Verhasselt

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Background: Abnormal gut barrier function is the basis of gut inflammatory disease. It is known that house dust mite (HDM) aero-allergens induce inflammation in respiratory mucosa. We have recently reported allergen from Dermatophagoides pteronyssinus (Der p1) to be present in rodent gut. Objective: To examine whether der p1 is present in human gut and to assess its effect on gut barrier function and inflammation. Design: Colonic biopsies, gut fluid, serum and stool were collected from healthy adults during endoscopy. der p1 was measured by ELISA. Effect of HDM was assessed on gut permeability, tight-junction and mucin expression, and cytokine production, in presence or absence of cysteine protease inhibitors or serine protease inhibitors. In vivo effect of HDM was examined in mice given oral HDM or protease-neutralised HDM. Role of HDM in low-grade inflammation was studied in patients with IBS. Results: HDM der p1 was detected in the human gut. In colonic biopsies from healthy patients, HDM increased epithelial permeability (p<0.001), reduced expression of tight-junction proteins and mucus barrier. These effects were associated with increased tumour necrosis factor (TNF)-α and interleukin (IL)-10 production and were abolished by cysteine-protease inhibitor (p<0.01). HDM effects did not require Th2 immunity. Results were confirmed in vivo in mice. In patients with IBS, HDM further deteriorated gut barrier function, induced TNF-α but failed to induce IL-10 secretion (p<0.001). Conclusions: HDM, a ubiquitous environmental factor, is present in the human gut where it directly affects gut function through its proteolytic activity. HDM may be an important trigger of gut dysfunction and warrants further investigation.

Original languageEnglish
Pages (from-to)757-766
Number of pages10
JournalGut
Volume65
Issue number5
DOIs
Publication statusPublished - 1 May 2016
Externally publishedYes

Fingerprint

Dermatophagoides Antigens
Pyroglyphidae
Gastrointestinal Tract
Cysteine Proteinase Inhibitors
Inflammation
Interleukin-10
Permeability
Tumor Necrosis Factor-alpha
Tight Junction Proteins
Biopsy
Respiratory Mucosa
Serine Proteinase Inhibitors
Tight Junctions
Mucins
Mucus
Endoscopy
Immunity
Rodentia
Peptide Hydrolases
Enzyme-Linked Immunosorbent Assay

Cite this

Tulic, Meri K. ; Vivinus-Nébot, Mylene ; Rekima, Akila ; Medeiros, Samara Rabelo ; Bonnart, Chrystelle ; Shi, Haining ; Walker, Allan ; Dainese, Raffaella ; Boyer, Julien ; Vergnolle, Nathalie ; Piche, Thierry ; Verhasselt, Valérie. / Presence of commensal house dust mite allergen in human gastrointestinal tract : A potential contributor to intestinal barrier dysfunction. In: Gut. 2016 ; Vol. 65, No. 5. pp. 757-766.
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abstract = "Background: Abnormal gut barrier function is the basis of gut inflammatory disease. It is known that house dust mite (HDM) aero-allergens induce inflammation in respiratory mucosa. We have recently reported allergen from Dermatophagoides pteronyssinus (Der p1) to be present in rodent gut. Objective: To examine whether der p1 is present in human gut and to assess its effect on gut barrier function and inflammation. Design: Colonic biopsies, gut fluid, serum and stool were collected from healthy adults during endoscopy. der p1 was measured by ELISA. Effect of HDM was assessed on gut permeability, tight-junction and mucin expression, and cytokine production, in presence or absence of cysteine protease inhibitors or serine protease inhibitors. In vivo effect of HDM was examined in mice given oral HDM or protease-neutralised HDM. Role of HDM in low-grade inflammation was studied in patients with IBS. Results: HDM der p1 was detected in the human gut. In colonic biopsies from healthy patients, HDM increased epithelial permeability (p<0.001), reduced expression of tight-junction proteins and mucus barrier. These effects were associated with increased tumour necrosis factor (TNF)-α and interleukin (IL)-10 production and were abolished by cysteine-protease inhibitor (p<0.01). HDM effects did not require Th2 immunity. Results were confirmed in vivo in mice. In patients with IBS, HDM further deteriorated gut barrier function, induced TNF-α but failed to induce IL-10 secretion (p<0.001). Conclusions: HDM, a ubiquitous environmental factor, is present in the human gut where it directly affects gut function through its proteolytic activity. HDM may be an important trigger of gut dysfunction and warrants further investigation.",
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Tulic, MK, Vivinus-Nébot, M, Rekima, A, Medeiros, SR, Bonnart, C, Shi, H, Walker, A, Dainese, R, Boyer, J, Vergnolle, N, Piche, T & Verhasselt, V 2016, 'Presence of commensal house dust mite allergen in human gastrointestinal tract: A potential contributor to intestinal barrier dysfunction' Gut, vol. 65, no. 5, pp. 757-766. https://doi.org/10.1136/gutjnl-2015-310523

Presence of commensal house dust mite allergen in human gastrointestinal tract : A potential contributor to intestinal barrier dysfunction. / Tulic, Meri K.; Vivinus-Nébot, Mylene; Rekima, Akila; Medeiros, Samara Rabelo; Bonnart, Chrystelle; Shi, Haining; Walker, Allan; Dainese, Raffaella; Boyer, Julien; Vergnolle, Nathalie; Piche, Thierry; Verhasselt, Valérie.

In: Gut, Vol. 65, No. 5, 01.05.2016, p. 757-766.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Presence of commensal house dust mite allergen in human gastrointestinal tract

T2 - A potential contributor to intestinal barrier dysfunction

AU - Tulic, Meri K.

AU - Vivinus-Nébot, Mylene

AU - Rekima, Akila

AU - Medeiros, Samara Rabelo

AU - Bonnart, Chrystelle

AU - Shi, Haining

AU - Walker, Allan

AU - Dainese, Raffaella

AU - Boyer, Julien

AU - Vergnolle, Nathalie

AU - Piche, Thierry

AU - Verhasselt, Valérie

PY - 2016/5/1

Y1 - 2016/5/1

N2 - Background: Abnormal gut barrier function is the basis of gut inflammatory disease. It is known that house dust mite (HDM) aero-allergens induce inflammation in respiratory mucosa. We have recently reported allergen from Dermatophagoides pteronyssinus (Der p1) to be present in rodent gut. Objective: To examine whether der p1 is present in human gut and to assess its effect on gut barrier function and inflammation. Design: Colonic biopsies, gut fluid, serum and stool were collected from healthy adults during endoscopy. der p1 was measured by ELISA. Effect of HDM was assessed on gut permeability, tight-junction and mucin expression, and cytokine production, in presence or absence of cysteine protease inhibitors or serine protease inhibitors. In vivo effect of HDM was examined in mice given oral HDM or protease-neutralised HDM. Role of HDM in low-grade inflammation was studied in patients with IBS. Results: HDM der p1 was detected in the human gut. In colonic biopsies from healthy patients, HDM increased epithelial permeability (p<0.001), reduced expression of tight-junction proteins and mucus barrier. These effects were associated with increased tumour necrosis factor (TNF)-α and interleukin (IL)-10 production and were abolished by cysteine-protease inhibitor (p<0.01). HDM effects did not require Th2 immunity. Results were confirmed in vivo in mice. In patients with IBS, HDM further deteriorated gut barrier function, induced TNF-α but failed to induce IL-10 secretion (p<0.001). Conclusions: HDM, a ubiquitous environmental factor, is present in the human gut where it directly affects gut function through its proteolytic activity. HDM may be an important trigger of gut dysfunction and warrants further investigation.

AB - Background: Abnormal gut barrier function is the basis of gut inflammatory disease. It is known that house dust mite (HDM) aero-allergens induce inflammation in respiratory mucosa. We have recently reported allergen from Dermatophagoides pteronyssinus (Der p1) to be present in rodent gut. Objective: To examine whether der p1 is present in human gut and to assess its effect on gut barrier function and inflammation. Design: Colonic biopsies, gut fluid, serum and stool were collected from healthy adults during endoscopy. der p1 was measured by ELISA. Effect of HDM was assessed on gut permeability, tight-junction and mucin expression, and cytokine production, in presence or absence of cysteine protease inhibitors or serine protease inhibitors. In vivo effect of HDM was examined in mice given oral HDM or protease-neutralised HDM. Role of HDM in low-grade inflammation was studied in patients with IBS. Results: HDM der p1 was detected in the human gut. In colonic biopsies from healthy patients, HDM increased epithelial permeability (p<0.001), reduced expression of tight-junction proteins and mucus barrier. These effects were associated with increased tumour necrosis factor (TNF)-α and interleukin (IL)-10 production and were abolished by cysteine-protease inhibitor (p<0.01). HDM effects did not require Th2 immunity. Results were confirmed in vivo in mice. In patients with IBS, HDM further deteriorated gut barrier function, induced TNF-α but failed to induce IL-10 secretion (p<0.001). Conclusions: HDM, a ubiquitous environmental factor, is present in the human gut where it directly affects gut function through its proteolytic activity. HDM may be an important trigger of gut dysfunction and warrants further investigation.

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DO - 10.1136/gutjnl-2015-310523

M3 - Article

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EP - 766

JO - Gut: an international journal of gastroenterology & hepatology

JF - Gut: an international journal of gastroenterology & hepatology

SN - 0017-5749

IS - 5

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