Preparation, biodistribution and stability of [65ZN]bleomycin complex

Amir Reza Jalilian; Pejman Rowshanfarzad; Ali Rahiminejad-Kisomi; Sedigheh Moradkhani; Farahnaz Motamedi-Sedeh

Preparation, biodistribution and stability of [⁶⁵ZN]bleomycin complex

Amir Reza Jalilian
, Pejman Rowshanfarzad
, Ali Rahiminejad-Kisomi
, Sedigheh Moradkhani
, Farahnaz Motamedi-Sedeh

School of Physics, Mathematics and Computing

Research output: Contribution to journal › Article › peer-review

Abstract

Bleomycin (BLM) has been labeled with various radioisotopes and widely used in therapy and diagnosis. In this study BLM was labeled with [⁶⁵Zn] zinc chloride and its distribution and stability in normal and tumor bearing mice was determined. The complex was obtained at the pH=2 in normal saline at 90 °C in 60 minutes. Radio-TLC showed an overall radiochemical yield of 95-97% (radiochemical purity >97%). The in vitro stability of the complex was determined in mice and human plasma. Preliminary studies were performed to determine distribution of [⁶⁵Zn]BLM in normal and tumor bearing mice on the basis of these results. [⁶⁵Zn]BLM may be used in therapeutic studies due to its suitable physico-chemical properties.

Original language	English
Pages (from-to)	115-122
Number of pages	8
Journal	Daru
Volume	12
Issue number	3
Publication status	Published - 17 Dec 2004

Cite this

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title = "Preparation, biodistribution and stability of [65ZN]bleomycin complex",

abstract = "Bleomycin (BLM) has been labeled with various radioisotopes and widely used in therapy and diagnosis. In this study BLM was labeled with [65Zn] zinc chloride and its distribution and stability in normal and tumor bearing mice was determined. The complex was obtained at the pH=2 in normal saline at 90 °C in 60 minutes. Radio-TLC showed an overall radiochemical yield of 95-97\% (radiochemical purity >97\%). The in vitro stability of the complex was determined in mice and human plasma. Preliminary studies were performed to determine distribution of [65Zn]BLM in normal and tumor bearing mice on the basis of these results. [65Zn]BLM may be used in therapeutic studies due to its suitable physico-chemical properties.",

keywords = "Biodistribution, Bleomycins, Cyclotron, Euger emitters, Zinc-65",

author = "Jalilian, \{Amir Reza\} and Pejman Rowshanfarzad and Ali Rahiminejad-Kisomi and Sedigheh Moradkhani and Farahnaz Motamedi-Sedeh",

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T1 - Preparation, biodistribution and stability of [65ZN]bleomycin complex

AU - Jalilian, Amir Reza

AU - Rowshanfarzad, Pejman

AU - Rahiminejad-Kisomi, Ali

AU - Moradkhani, Sedigheh

AU - Motamedi-Sedeh, Farahnaz

PY - 2004/12/17

Y1 - 2004/12/17

N2 - Bleomycin (BLM) has been labeled with various radioisotopes and widely used in therapy and diagnosis. In this study BLM was labeled with [65Zn] zinc chloride and its distribution and stability in normal and tumor bearing mice was determined. The complex was obtained at the pH=2 in normal saline at 90 °C in 60 minutes. Radio-TLC showed an overall radiochemical yield of 95-97% (radiochemical purity >97%). The in vitro stability of the complex was determined in mice and human plasma. Preliminary studies were performed to determine distribution of [65Zn]BLM in normal and tumor bearing mice on the basis of these results. [65Zn]BLM may be used in therapeutic studies due to its suitable physico-chemical properties.

AB - Bleomycin (BLM) has been labeled with various radioisotopes and widely used in therapy and diagnosis. In this study BLM was labeled with [65Zn] zinc chloride and its distribution and stability in normal and tumor bearing mice was determined. The complex was obtained at the pH=2 in normal saline at 90 °C in 60 minutes. Radio-TLC showed an overall radiochemical yield of 95-97% (radiochemical purity >97%). The in vitro stability of the complex was determined in mice and human plasma. Preliminary studies were performed to determine distribution of [65Zn]BLM in normal and tumor bearing mice on the basis of these results. [65Zn]BLM may be used in therapeutic studies due to its suitable physico-chemical properties.

KW - Biodistribution

KW - Bleomycins

KW - Cyclotron

KW - Euger emitters

KW - Zinc-65

UR - https://www.scopus.com/pages/publications/10044258479

M3 - Article

SN - 1560-8115

VL - 12

SP - 115

EP - 122

JO - Daru

JF - Daru

IS - 3

ER -

Preparation, biodistribution and stability of [⁶⁵ZN]bleomycin complex

Abstract

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