Preparation and preliminary evaluation of [55Co](II)vancomycin

Amir Reza Jalilian, Yousef Yari-Kamrani, Pejman Rowshanfarzad, Mahseed Sabet, Mohsen Kamali-Dehghan, Abbas Majdabadi

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Abstract

Co-55 (t1/2=17.53 h) was produced by 150 μA irradiation of a natural nickei target using 15 MeV protons. It was separated from the irradiated target material by two ion exchange chromatography steps with a radiochemical yield of >95% and was used for the preparation of [55Co]vancomycin ([55Co]VAN). Optimization studies were performed using Co-57 due to its longer half-life. Cobalt-57 (t1/2=271.79 d) was produced by irradiation of a natural nickel target with 150 μA current of 22 MeV protons. The 57Co was separated from the irradiated target material using a no-carrier-added method with a radiochemical yield of >97%. Both products were controlled for radionuclide and chemical purity. The solutions of [55Co]VAN were prepared (radiochemical yield >80%) starting with 55Co acetate and vancomycin at room temperature after 30 min. A precise solid phrase extraction (SPE) method was developed using Si Sep-Pak in order to purify/reconstitute the final formulation for animal studies. [55Co]VAN showed a radiochemical purity of more than 99%. The resultant specific activity was about 1.15 TBq/mmol. It is proved that the tracer is stable in the final product and in presence of human serum at 37°C up to 24 h. Biodistribution study of [55Co]VAN in normal rats was undertaken for up to 72 h.

Original languageEnglish
Pages (from-to)347-353
Number of pages7
JournalNuclear science and techniques
Volume19
Issue number6
DOIs
Publication statusPublished - 20 Dec 2008

Cite this

Jalilian, A. R., Yari-Kamrani, Y., Rowshanfarzad, P., Sabet, M., Kamali-Dehghan, M., & Majdabadi, A. (2008). Preparation and preliminary evaluation of [55Co](II)vancomycin. Nuclear science and techniques, 19(6), 347-353. https://doi.org/10.1016/S1001-8042(09)60017-8