Preparation and evaluation of [61Cu]-thiophene-2-aldehyde thiosemicarbazone for PET studies

Amir Reza Jalilian, Mirsaeed Nikzad, Hassan Zandi, Ali Nemati Kharat, Pejman Rowshanfarzad, Mehdi Akhlaghi, Fatemeh Bolourinovin, A. R. Jalilian

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

BACKGROUND: [61Cu]Thiophene-2-aldehyde thiosemicarbazone ([ 61Cu]TATS) (4) was prepared according to an analogy of carrier copper compound with antitumor activity, for eventual use in PET. MATERIAL AND METHODS: [61Cu]TATS was prepared using copper-61 acetate and in-house made ligand (TATS) in one step. 61Cu was produced via the natZn(p,x)61Cu nuclear reaction (180 μA, 22 MeV, 3.2 h) followed by a two-step chromatography method (222 GBq of 61Cu 2+). [61Cu]TATS preparation was optimized for reaction conditions (buffer concentration and temperature). The tracer was finally administered to normal rats for biodistribution studies. RESULTS: Total radiolabelling of the tracer took 30 minutes with a radiochemical purity of more than 90% (using HPLC and RTLC) and specific activity of about 250-300 Ci/mmol. The complex was stable in the presence of human serum for an hour. The biodistribution of copper cation and the tracer was checked in wild-type rats for up to 2 hours with significant spleen and lung uptake of the tracer. CONCLUSIONS: The production of 61Cu via the natZn(p,x) 61Cu is an efficient and reproducible method with high specific activity leading to the production and preliminary evaluation of [ 61Cu]TATS, a potential PET tracer, was reported.

Original languageEnglish
Pages (from-to)41-47
Number of pages7
JournalNuclear Medicine Review
Volume11
Issue number2
Publication statusPublished - 1 Dec 2008

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