Preparation and biological evaluation of a [ 55Co]-2- acetylpyridine thiosemicarbazone

Amir R. Jalilian, Pejman Rowshanfarzad, Mehdi Akhlaghi, Mahsheed Sabet, Mohsen Kamali-Dehghan, Mehrban Pouladi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Due to the anti-proliferative properties of cobalt-thiosemicarbazone complexes, the production of [ 55Co](III)-bis-(2-acetylpyridine thiosemicarbazone) ([ 55Co](III)[APTS] 2) was investigated. Co-55 (T 1/2=17.53 h) was produced by 150 μA irradiation of a natural nickel target by 15 MeV protons. The 55Co was separated from the irradiated target material using a two-step method with a radiochemical yield of >95% followed by radionuclidic and chemical purity control. [ 55Co](III)chloride was mixed with 2-acetylpyridine thiosemicarbazone for 30 min at room temperature to yield [ 55Co](III)[APTS] 2 (radiochemical purity > 98% shown by RTLC/HPLC). A specific activity of about 10-20 Ci/mmol was obtained. The final solution was diluted in normal saline to 5% ethanolic solution for biological evaluation. The stability of the final product was checked in the absence and presence of human serum at 37°C to 24 h. The partition co-efficient of the final complex at the pH of 7 was 1.00±0.08. A significant tumor accumulation (%ID/g; 3.5%) was observed in tumoral tissue 21 h post injection in fibrosarcoma-bearing mice by biodistribution studies. Co-incidence imaging also demonstrated tumor uptake from 21-35 h however at 35 h tumor uptake is more specific and significant.

Original languageEnglish
Pages (from-to)567-578
Number of pages12
JournalScientia Pharmaceutica
Volume77
Issue number3
DOIs
Publication statusPublished - 25 Sep 2009
Externally publishedYes

Fingerprint Dive into the research topics of 'Preparation and biological evaluation of a [ <sup>55</sup>Co]-2- acetylpyridine thiosemicarbazone'. Together they form a unique fingerprint.

Cite this