Predicting the binding specificity of PLS-PPR RNA editing factors in plant organelles

Michael Roderic Charles Millman

Research output: ThesisMaster's Thesis

96 Downloads (Pure)

Abstract

C-to-U RNA editing events are prevalent in land plant organelles. Specificity of these events is mediated by PPR proteins, which recognize and bind RNA sequences via their PPR motifs. I performed RNA-seq on two mutant Arabidopsis lines to identify mitochondrial editing events mediated by the PPR proteins AT1G13410 (MEF134) and BIR1 (AT3G61170) respectively. Restoration of editing at these sites was confirmed in mutants complemented by Agrobacterium floral dip transformation. MEF134 is required for editing of nad5-553 and nad3-352. BIR1 is required for editing of the nad1-493, nad4-403, nad7-698 and ccmFN2-356 sites.
Original languageEnglish
QualificationMasters
Awarding Institution
  • The University of Western Australia
Thesis sponsors
Award date4 Aug 2018
DOIs
Publication statusUnpublished - 2018

Fingerprint Dive into the research topics of 'Predicting the binding specificity of PLS-PPR RNA editing factors in plant organelles'. Together they form a unique fingerprint.

Cite this