TY - JOUR
T1 - Precision betacarotene supplementation enhanced ovarian function and the LH release pattern in yearling crossbred anestrous goats
AU - Lopez-Flores, Noé M.
AU - Meza-Herrera, César A.
AU - Perez-Marin, C.
AU - Blache, Dominique
AU - Arellano-Rodríguez, Gerardo
AU - Zuñiga-Garcia, Santiago
AU - Navarrete-Molina, Cayetano
AU - De La Peña, Cristina García
AU - Rosales-Nieto, Cesar A.
AU - Veliz-Deras, Francisco G.
PY - 2020/4
Y1 - 2020/4
N2 - The potential out-of-season supplementation effect of beta-carotene (BETA) upon both ovarian function and the luteinizing hormone LH release pattern in anestrous yearling goats was evaluated. Oral BETA-supply to goats with an enlarged gene-pool of dairy goat breeds, influenced in a positive fashion, the out-of-season ovarian outcomes escorted by changes in the release pattern of LH regarding the Control group. Results denote a likely role of BETA not only as modulator ovarian function measured as ovulation rate and antral follicular number but also involved as modifier of the LH-release pattern. The obtained outcomes would be central in the proposal of out-of-season reproductive schemes to enhance the ovarian outcomes in crossbred dairy goats. The possible out-of-season effect of beta-carotene supplementation on ovulation rate (OR), antral follicles (AFN), and total ovarian activity (TOA = OR + AFN) as related to the LH release pattern in yearling anestrous goats was evaluated. In late April, Alpine-Saanen-Nubian x Criollo goats (n = 22, 26 N) were randomly allotted to: (1) Beta-carotene (BETA; n = 10, orally supplemented with 50 mg/goat/d; 36.4 ± 1.07 kg live weight (LW), 3.5 ± 0.20 units, body condition score (BCS) or (2) Non-supplemented (CONT; n = 12, 35.2 ± 1.07 kg LW, 3.4 ± 0.2 units BCS). Upon estrus synchronization, an intensive blood sampling (6 h × 15 min) was accomplished in May for LH quantifications; response variables included (pulsatility-PULSE, time to first pulse-TTFP, amplitude-AMPL, nadir-NAD and area under the curve-AUC). Thereafter, an ultrasonography scanning was completed to assess OR and AFN. The Munro algorithm was used to quantify LH pulsatility; if significant effects of time, treatment or interaction were identified, data were compared across time. Neither LW nor BCS (p > 0.05) or even the LH (p > 0.05); PULSE (4.1 ± 0.9 pulses/6 h), NAD (0.47 ± 0.13 ng) and AUC (51.7 ± 18.6 units) differed between treatments. Nonetheless, OR (1.57 vs. 0.87 ± 0.18 units) and TOA (3.44 vs. 1.87 ± 0.45 units) escorted by a reduced TTFP (33 vs. 126 ± 31.9 min) and an increased AMPL (0.55 vs. 0.24 ± 0.9 ng), favored to the BETA supplemented group (p < 0.05), possibly through a GnRH-LH enhanced pathway and(or) a direct effect at ovarian level. Results are relevant to speed-up the out-of-season reproductive outcomes in goats while may embrace translational applications.
AB - The potential out-of-season supplementation effect of beta-carotene (BETA) upon both ovarian function and the luteinizing hormone LH release pattern in anestrous yearling goats was evaluated. Oral BETA-supply to goats with an enlarged gene-pool of dairy goat breeds, influenced in a positive fashion, the out-of-season ovarian outcomes escorted by changes in the release pattern of LH regarding the Control group. Results denote a likely role of BETA not only as modulator ovarian function measured as ovulation rate and antral follicular number but also involved as modifier of the LH-release pattern. The obtained outcomes would be central in the proposal of out-of-season reproductive schemes to enhance the ovarian outcomes in crossbred dairy goats. The possible out-of-season effect of beta-carotene supplementation on ovulation rate (OR), antral follicles (AFN), and total ovarian activity (TOA = OR + AFN) as related to the LH release pattern in yearling anestrous goats was evaluated. In late April, Alpine-Saanen-Nubian x Criollo goats (n = 22, 26 N) were randomly allotted to: (1) Beta-carotene (BETA; n = 10, orally supplemented with 50 mg/goat/d; 36.4 ± 1.07 kg live weight (LW), 3.5 ± 0.20 units, body condition score (BCS) or (2) Non-supplemented (CONT; n = 12, 35.2 ± 1.07 kg LW, 3.4 ± 0.2 units BCS). Upon estrus synchronization, an intensive blood sampling (6 h × 15 min) was accomplished in May for LH quantifications; response variables included (pulsatility-PULSE, time to first pulse-TTFP, amplitude-AMPL, nadir-NAD and area under the curve-AUC). Thereafter, an ultrasonography scanning was completed to assess OR and AFN. The Munro algorithm was used to quantify LH pulsatility; if significant effects of time, treatment or interaction were identified, data were compared across time. Neither LW nor BCS (p > 0.05) or even the LH (p > 0.05); PULSE (4.1 ± 0.9 pulses/6 h), NAD (0.47 ± 0.13 ng) and AUC (51.7 ± 18.6 units) differed between treatments. Nonetheless, OR (1.57 vs. 0.87 ± 0.18 units) and TOA (3.44 vs. 1.87 ± 0.45 units) escorted by a reduced TTFP (33 vs. 126 ± 31.9 min) and an increased AMPL (0.55 vs. 0.24 ± 0.9 ng), favored to the BETA supplemented group (p < 0.05), possibly through a GnRH-LH enhanced pathway and(or) a direct effect at ovarian level. Results are relevant to speed-up the out-of-season reproductive outcomes in goats while may embrace translational applications.
KW - Beta-carotene
KW - Goats
KW - Reproductive efficiency
KW - Targeted supplementation
UR - http://www.scopus.com/inward/record.url?scp=85083725393&partnerID=8YFLogxK
U2 - 10.3390/ani10040659
DO - 10.3390/ani10040659
M3 - Article
C2 - 32290230
AN - SCOPUS:85083725393
SN - 2076-2615
VL - 10
JO - Animals
JF - Animals
IS - 4
M1 - 659
ER -