Prebiotic oligosaccharides in early life alter gut microbiome development in male mice while supporting influenza vaccination responses

L W J van den Elsen, S Tims, A M Jones, A Stewart, B Stahl, J Garssen, J Knol, E E Forbes-Blom, B Van't Land

Research output: Contribution to journalArticle

Abstract

Beneficial modulation of the gut microbiota is an attractive therapeutic approach to improve the efficacy of vaccine-induced immunity. In this study, mice were supplemented with the prebiotic milk oligosaccharide 2'-fucosyllactose (2'FL) as well as a complex mixture of immune modulatory prebiotic short-chain galacto-oligosaccharides and long-chain fructo-oligosaccharides (scGOS/lcFOS) from different stages in early life. Adult mice were vaccinated with trivalent influenza vaccine (TIV) and both development of the gut microbiota and antibody-mediated vaccine responses were followed over time. Within the control group, female mice demonstrated a larger antibody response to TIV vaccination than male mice, which was accompanied by enhanced cytokine production by splenocytes and a higher percentage of plasma cells in skin draining lymph nodes. In addition, the prebiotic diet improved vaccine-specific antibody responses in male mice. Introduction of prebiotics into the diet modulated the gut microbiota composition and at the genus level several bacterial groups showed a significant interaction effect which potentially contributed to the immunological effects observed. This study provides insight in the effect of scGOS/lcFOS/2'FL in influenza vaccination antibody production.

Original languageEnglish
Pages (from-to)279-291
Number of pages14
JournalBeneficial Microbes
Volume10
Issue number3
DOIs
Publication statusPublished - 18 Feb 2019
Externally publishedYes

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Prebiotics
Oligosaccharides
Human Influenza
Vaccination
Antibody Formation
Vaccines
Influenza Vaccines
Diet
Plasma Cells
Complex Mixtures
Immunity
Milk
Lymph Nodes
Gastrointestinal Microbiome
Cytokines
Control Groups
Skin
Antibodies

Cite this

van den Elsen, L W J ; Tims, S ; Jones, A M ; Stewart, A ; Stahl, B ; Garssen, J ; Knol, J ; Forbes-Blom, E E ; Van't Land, B. / Prebiotic oligosaccharides in early life alter gut microbiome development in male mice while supporting influenza vaccination responses. In: Beneficial Microbes. 2019 ; Vol. 10, No. 3. pp. 279-291.
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Prebiotic oligosaccharides in early life alter gut microbiome development in male mice while supporting influenza vaccination responses. / van den Elsen, L W J; Tims, S; Jones, A M; Stewart, A; Stahl, B; Garssen, J; Knol, J; Forbes-Blom, E E; Van't Land, B.

In: Beneficial Microbes, Vol. 10, No. 3, 18.02.2019, p. 279-291.

Research output: Contribution to journalArticle

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