Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 361-368 |
| Journal | Experimental Neurology |
| Volume | 193 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2005 |
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Post-ischemic modest hypothermia (35°C) combined with intravenous magnesium is more effective at reducing CA1 neuronal death than either treatment used alone following global cerebral ischemia in rats. / Zhu, H.; Meloni, Bruno; Bojarski, Christina; Knuckey, M.W.; Knuckey, Neville.
In: Experimental Neurology, Vol. 193, No. 2, 2005, p. 361-368.Research output: Contribution to journal › Article
TY - JOUR
T1 - Post-ischemic modest hypothermia (35°C) combined with intravenous magnesium is more effective at reducing CA1 neuronal death than either treatment used alone following global cerebral ischemia in rats
AU - Zhu, H.
AU - Meloni, Bruno
AU - Bojarski, Christina
AU - Knuckey, M.W.
AU - Knuckey, Neville
PY - 2005
Y1 - 2005
N2 - In this study, we investigated the efficacy of pre- and 2 h post-ischemic magnesium treatment with different durations of modest hypothermia (35°C) induced immediately or 2 h following global cerebral ischemia in rats. In experimental group 1, rats received an intravenous loading dose (LD) of 360 μmol/kg MgSO4 immediately before ischemia followed by a 48 h intravenous infusion (IVI) at 120 μmol/kg/h. Immediately post-ischemia, body temperature was lowered to 35°C for 6 h or maintained at 37°C. In experimental group 2, 2 h after ischemia, rats received the MgSO4 LD/IVI and/or had their body temperature lowered to 35°C for 6, 12 or 24 h. In experimental group 1, ischemic rats receiving 6 h of modest hypothermia demonstrated 9.4% CA1 neuronal survival, whereas rats treated with magnesium alone or magnesium and 6 h of modest hypothermia demonstrated 5.1% and 37.9% neuronal survival, respectively. In experimental group 2, ischemic rats receiving 6, 12 or 24 h of modest hypothermia demonstrated 6.1, 5 and 43% CA1 neuronal survival, respectively. Rats treated with magnesium and 6, 12 or 24 h of modest hypothermia demonstrated 8.1, 9 and 76% neuronal survival, respectively. Our findings demonstrate that post-ischemic treatment with a 24 h duration of modest hypothermia and magnesium is more effective than either treatment used alone.
AB - In this study, we investigated the efficacy of pre- and 2 h post-ischemic magnesium treatment with different durations of modest hypothermia (35°C) induced immediately or 2 h following global cerebral ischemia in rats. In experimental group 1, rats received an intravenous loading dose (LD) of 360 μmol/kg MgSO4 immediately before ischemia followed by a 48 h intravenous infusion (IVI) at 120 μmol/kg/h. Immediately post-ischemia, body temperature was lowered to 35°C for 6 h or maintained at 37°C. In experimental group 2, 2 h after ischemia, rats received the MgSO4 LD/IVI and/or had their body temperature lowered to 35°C for 6, 12 or 24 h. In experimental group 1, ischemic rats receiving 6 h of modest hypothermia demonstrated 9.4% CA1 neuronal survival, whereas rats treated with magnesium alone or magnesium and 6 h of modest hypothermia demonstrated 5.1% and 37.9% neuronal survival, respectively. In experimental group 2, ischemic rats receiving 6, 12 or 24 h of modest hypothermia demonstrated 6.1, 5 and 43% CA1 neuronal survival, respectively. Rats treated with magnesium and 6, 12 or 24 h of modest hypothermia demonstrated 8.1, 9 and 76% neuronal survival, respectively. Our findings demonstrate that post-ischemic treatment with a 24 h duration of modest hypothermia and magnesium is more effective than either treatment used alone.
U2 - 10.1016/j.expneurol.2005.01.022
DO - 10.1016/j.expneurol.2005.01.022
M3 - Article
VL - 193
SP - 361
EP - 368
JO - Experimental Neurology
JF - Experimental Neurology
SN - 0014-4886
IS - 2
ER -