Polycomb-mediated chromatin loops revealed by a subkilobase-resolution chromatin interaction map

Kyle P. Eagen, Erez Lieberman Aiden, Roger D. Kornberg

Research output: Contribution to journalArticlepeer-review

89 Citations (Web of Science)

Abstract

The locations of chromatin loops in Drosophila were determined by Hi-C (chemical cross-linking, restriction digestion, ligation, and high-throughput DNA sequencing). Whereas most loop boundaries or “anchors” are associated with CTCF protein in mammals, loop anchors in Drosophila were found most often in association with the polycomb group (PcG) protein Polycomb (Pc), a subunit of polycomb repressive complex 1 (PRC1). Loops were frequently located within domains of PcG-repressed chromatin. Promoters located at PRC1 loop anchors regulate some of the most important developmental genes and are less likely to be expressed than those not at PRC1 loop anchors. Although DNA looping has most commonly been associated with enhancer–promoter communication, our results indicate that loops are also associated with gene repression.

Original languageEnglish
Pages (from-to)8764-8769
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume114
Issue number33
DOIs
Publication statusPublished - 15 Aug 2017
Externally publishedYes

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