Polyclonal antibodies in livestock for treatment of ingested toxin in human

Shreen Nusair

    Research output: ThesisDoctoral Thesis

    362 Downloads (Pure)

    Abstract

    [Truncated] The effectiveness of antibodies for neutralizing foreign substances has been well established since 1870. Behring and Kitasato were the first to describe the use of antibodies when they treated diphtheria with an antiserum from rabbits raised against diphtheria toxin. In more recent times, antibodies have been engineered to treat a whole spectrum of illnesses including asthma, different types of cancer (e.g. bladder cancer, leukemia and other congenital blood disorders), bacterial infection (e.g. Staphylococcus aureus infection), viral infections (e.g. hepatitis B and rabies) and bites resulting in the injection of venoms (e.g. brown snake envenomation). In addition, antibodies have many applications in biomedical, industrial and forensic investigations. In forensic science, antibodies have been used to investigate biological evidences (e.g. blood and saliva) which are collected at crime scenes. For example, using the ability of antibodies to bind and agglutinate antigens present on the surface of red blood cells used commonly for biomedical applications. A-B-O blood grouping is a common example of tests which are used to identify blood collected at crime scenes. The method, A-B-O blood grouping is a simple yet effective tool.

    The series of studies described in this thesis were conceived to examine the use of polyclonal antibodies raised in the milk of sheep as a tool to treat poisoning episodes caused by three common agents; cocaine, amatoxin and deltamethrin were studied. The thesis is divided into three sections: (1) introduction, (2) findings of the research work undertaken and (3) conclusions with recommendations arising from the work completed. The introduction section includes four chapters; a review of the state of antibodies used for forensic applications; a published mini review which summarizes commonly found toxins and their medical and industrial applications; a published mini review of the pathological consequences in humans and animals due to exposure to common toxins; and published mini review of the available pharmacotherapy and immunotherapy to treat poisoning of common toxins.
    Original languageEnglish
    QualificationDoctor of Philosophy
    Publication statusUnpublished - 2012

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