PolI-driven integrative expression vectors for yeast.

Pilar Blancafort, Gerardo Ferbeyre , Carlos Sariol , Robert Cedergren

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A novel expression vector for yeast has been constructed from the regulatory elements present in the polI promoter and the enhancer/termination region (E/T) of rDNA. Under some conditions, this promoter/vector combination produces small RNAs such as the hammerhead RNA sequence at levels comparable to polII- and polIII-dependent systems. No stable transcription product can be demonstrated with this vector when the enhancer/termination sequence is less than 100 nucleotides downstream from the promoter. On the other hand, high expression of a stable, hammerhead RNA molecule can be obtained from this vector by inserting a 400-bp fragment containing the ADH1 transcription termination region upstream of the E/T. RNAs produced by this vector are polyadenylated and multiple copies of this plasmid can be stably integrated into the yeast chromosome.
Original languageEnglish
Pages (from-to)41-47
JournalJournal of Biotechnology
Volume56
Issue number1
Publication statusPublished - 1997

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