TY - JOUR
T1 - Point mutation impairs centromeric CENH3 loading and induces haploid plants
AU - Karimi-Ashtiyani, Raheleh
AU - Ishii, Takayoshi
AU - Niessen, Markus
AU - Stein, Nils
AU - Heckmann, Stefan
AU - Gurushidze, Maia
AU - Banaei-Moghaddam, Ali Mohammad
AU - Fuchs, Jörg
AU - Schubert, Veit
AU - Koch, Kerstin
AU - Weiss, Oda
AU - Demidov, Dmitri
AU - Schmidt, Klaus
AU - Kumlehn, Jochen
AU - Houben, Andreas
PY - 2015/9/8
Y1 - 2015/9/8
N2 - The chromosomal position of the centromere-specific histone H3 variant CENH3 (also called "CENP-A") is the assembly site for the kinetochore complex of active centromeres. Any error in transcription, translation, modification, or incorporation can affect the ability to assemble intact CENH3 chromatin and can cause centromere inactivation [Allshire RC, Karpen GH (2008) Nat Rev Genet 9(12):923-937]. Here we show that a single-point amino acid exchange in the centromere-targeting domain of CENH3 leads to reduced centromere loading of CENH3 in barley, sugar beet, and Arabidopsis thaliana. Haploids were obtained after cenh3 L130F-complemented cenh3-null mutant plants were crossed with wildtype A. thaliana. In contrast, in a noncompeting situation (i.e., centromeres possessing only mutated or only wild-type CENH3), no uniparental chromosome elimination occurs during early embryogenesis. The high degree of evolutionary conservation of the identified mutation site offers promising opportunities for application in a wide range of crop species in which haploid technology is of interest.
AB - The chromosomal position of the centromere-specific histone H3 variant CENH3 (also called "CENP-A") is the assembly site for the kinetochore complex of active centromeres. Any error in transcription, translation, modification, or incorporation can affect the ability to assemble intact CENH3 chromatin and can cause centromere inactivation [Allshire RC, Karpen GH (2008) Nat Rev Genet 9(12):923-937]. Here we show that a single-point amino acid exchange in the centromere-targeting domain of CENH3 leads to reduced centromere loading of CENH3 in barley, sugar beet, and Arabidopsis thaliana. Haploids were obtained after cenh3 L130F-complemented cenh3-null mutant plants were crossed with wildtype A. thaliana. In contrast, in a noncompeting situation (i.e., centromeres possessing only mutated or only wild-type CENH3), no uniparental chromosome elimination occurs during early embryogenesis. The high degree of evolutionary conservation of the identified mutation site offers promising opportunities for application in a wide range of crop species in which haploid technology is of interest.
KW - CENH3 loading
KW - CENH3 mutant
KW - Chromosome elimination
KW - Haploid induction
KW - Plant breeding
UR - http://www.scopus.com/inward/record.url?scp=84941243898&partnerID=8YFLogxK
U2 - 10.1073/pnas.1504333112
DO - 10.1073/pnas.1504333112
M3 - Article
C2 - 26294252
AN - SCOPUS:84941243898
VL - 112
SP - 11211
EP - 11216
JO - National Academy of Sciences, Proceedings
JF - National Academy of Sciences, Proceedings
SN - 0027-8424
IS - 36
ER -