Point mutation impairs centromeric CENH3 loading and induces haploid plants

Raheleh Karimi-Ashtiyani, Takayoshi Ishii, Markus Niessen, Nils Stein, Stefan Heckmann, Maia Gurushidze, Ali Mohammad Banaei-Moghaddam, Jörg Fuchs, Veit Schubert, Kerstin Koch, Oda Weiss, Dmitri Demidov, Klaus Schmidt, Jochen Kumlehn, Andreas Houben

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

The chromosomal position of the centromere-specific histone H3 variant CENH3 (also called "CENP-A") is the assembly site for the kinetochore complex of active centromeres. Any error in transcription, translation, modification, or incorporation can affect the ability to assemble intact CENH3 chromatin and can cause centromere inactivation [Allshire RC, Karpen GH (2008) Nat Rev Genet 9(12):923-937]. Here we show that a single-point amino acid exchange in the centromere-targeting domain of CENH3 leads to reduced centromere loading of CENH3 in barley, sugar beet, and Arabidopsis thaliana. Haploids were obtained after cenh3 L130F-complemented cenh3-null mutant plants were crossed with wildtype A. thaliana. In contrast, in a noncompeting situation (i.e., centromeres possessing only mutated or only wild-type CENH3), no uniparental chromosome elimination occurs during early embryogenesis. The high degree of evolutionary conservation of the identified mutation site offers promising opportunities for application in a wide range of crop species in which haploid technology is of interest.

Original languageEnglish
Pages (from-to)11211-11216
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume112
Issue number36
DOIs
Publication statusPublished - 8 Sep 2015
Externally publishedYes

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