TY - JOUR
T1 - PLGA nanoparticles loaded with quaternary ammonium silane and riboflavin for potential applications in adhesive dentistry
AU - Daood, Umer
AU - Balasankar, Meera Priyadarshini
AU - Ibrahim, Marrwa A.
AU - Pichika, Mallikarjuna Rao
AU - Mak, Kit Kay
AU - Fawzy, Amr S.
PY - 2021/3
Y1 - 2021/3
N2 - Aim: Novel k21 PLGA nanoparticles (nano-PLGA-RF/VE-TPGS0.50⁞k21) with synergistic effect of VE-TPGS with riboflavin-5-phospshate solution were used to analyze structural integrity, chemical interactions, antimicrobial resistance, mechanical properties as a delivery tool to acid-demineralized dentine-substrates. Materials and methods: Synthesized spherical nano-PLGA⋮RF/VE-TPGS0.50⁞k21 were assessed for drug-encapsulation and release, antibacterial-profile, MTT, molecular simulation for MMP-2 and MMP-9 and Raman spectroscopy. Results: k21 favoured increase in colloidal-size with positive zeta potential. Bands indicated k21 entrapment as nanoparticles were confined inside tubules with no effect on μTBS. XP-GScores were −2.383 and −4.383 for MMP-2 and -9. HYP liberation recorded with collagenase degradation resistance in nano-PLGA⋮RF/VE-TPGS0.50⁞k21 specimens. Conclusion: Nano-PLGA⋮RF/VE-TPGS0.50⁞k21 specimens exhibited superior antibacterial/antibiofilm effects against cariogenic biofilms after bonding-resins infiltration without adversely affecting bond strength.
AB - Aim: Novel k21 PLGA nanoparticles (nano-PLGA-RF/VE-TPGS0.50⁞k21) with synergistic effect of VE-TPGS with riboflavin-5-phospshate solution were used to analyze structural integrity, chemical interactions, antimicrobial resistance, mechanical properties as a delivery tool to acid-demineralized dentine-substrates. Materials and methods: Synthesized spherical nano-PLGA⋮RF/VE-TPGS0.50⁞k21 were assessed for drug-encapsulation and release, antibacterial-profile, MTT, molecular simulation for MMP-2 and MMP-9 and Raman spectroscopy. Results: k21 favoured increase in colloidal-size with positive zeta potential. Bands indicated k21 entrapment as nanoparticles were confined inside tubules with no effect on μTBS. XP-GScores were −2.383 and −4.383 for MMP-2 and -9. HYP liberation recorded with collagenase degradation resistance in nano-PLGA⋮RF/VE-TPGS0.50⁞k21 specimens. Conclusion: Nano-PLGA⋮RF/VE-TPGS0.50⁞k21 specimens exhibited superior antibacterial/antibiofilm effects against cariogenic biofilms after bonding-resins infiltration without adversely affecting bond strength.
KW - Bonding
KW - Dental biofilm
KW - Drug-loaded nanoparticles
KW - k21
KW - Quaternary ammonium silane
UR - http://www.scopus.com/inward/record.url?scp=85098148625&partnerID=8YFLogxK
U2 - 10.1016/j.ijadhadh.2020.102797
DO - 10.1016/j.ijadhadh.2020.102797
M3 - Article
AN - SCOPUS:85098148625
SN - 0143-7496
VL - 105
JO - International Journal of Adhesion and Adhesives
JF - International Journal of Adhesion and Adhesives
M1 - 102797
ER -