TY - JOUR
T1 - Plasma cytokines levels in aggressive and chronic periodontitis
AU - Mattuella, Letícia Grando
AU - Campagnaro, Milene Borges
AU - Vargas, Andréia Escosteguy
AU - Xavier, Léder Leal
AU - Oppermann, Rui Vicente
AU - Chies, José Artur Bogo
AU - Miranda, Letícia Algarves
PY - 2012/9/5
Y1 - 2012/9/5
N2 - OBJECTIVE: The present study evaluated the Th1/Th2 cytokine profile in plasma from healthy controls and different types of periodontitis patients.MATERIALS AND METHODS: The concentration of IL-2, IL-4, IL-5, IL-10, TNF-α and IFN-γ was determined in healthy controls (n = 18) and patients with chronic (n = 19) and aggressive periodontitis (n = 19) using a flow cytometric multiplex immunoassay. Means and standard deviations were calculated and compared using Kruskal-Wallis test. Spearman rho coefficient was used to correlate cytokines in the studied groups.RESULTS: Although there was no significant difference in the concentration of cytokines between groups, there was a tendency to lower levels of IL-5 and IL-10 in the aggressive periodontitis group. Stronger correlations were observed between IL-2/IL-4 and IL-2/IL-10 in healthy controls (0.938 and 0.669, respectively) compared with chronic (0.746 and 0.532) and aggressive periodontitis groups (0.395 and 0.266). When compared to healthy (0.812) and chronic periodontitis (0.845) groups, the correlation of IL-4/IL-5 was weaker in the aggressive group (0.459).CONCLUSION: No difference between systemic levels of Th1/Th2 was observed. In aggressive periodontitis patients, nevertheless, a trend towards low levels of Th2 cytokines could suggest a contribution to the development of such an exacerbated manifestation of this disease.
AB - OBJECTIVE: The present study evaluated the Th1/Th2 cytokine profile in plasma from healthy controls and different types of periodontitis patients.MATERIALS AND METHODS: The concentration of IL-2, IL-4, IL-5, IL-10, TNF-α and IFN-γ was determined in healthy controls (n = 18) and patients with chronic (n = 19) and aggressive periodontitis (n = 19) using a flow cytometric multiplex immunoassay. Means and standard deviations were calculated and compared using Kruskal-Wallis test. Spearman rho coefficient was used to correlate cytokines in the studied groups.RESULTS: Although there was no significant difference in the concentration of cytokines between groups, there was a tendency to lower levels of IL-5 and IL-10 in the aggressive periodontitis group. Stronger correlations were observed between IL-2/IL-4 and IL-2/IL-10 in healthy controls (0.938 and 0.669, respectively) compared with chronic (0.746 and 0.532) and aggressive periodontitis groups (0.395 and 0.266). When compared to healthy (0.812) and chronic periodontitis (0.845) groups, the correlation of IL-4/IL-5 was weaker in the aggressive group (0.459).CONCLUSION: No difference between systemic levels of Th1/Th2 was observed. In aggressive periodontitis patients, nevertheless, a trend towards low levels of Th2 cytokines could suggest a contribution to the development of such an exacerbated manifestation of this disease.
KW - Case-Control Studies
KW - Chronic Disease
KW - Cytokines/blood
KW - Female
KW - Humans
KW - Infant, Newborn
KW - Male
KW - Middle Aged
KW - Periodontitis/blood
U2 - 10.3109/00016357.2012.715191
DO - 10.3109/00016357.2012.715191
M3 - Article
C2 - 22943212
VL - 71
SP - 683
EP - 688
JO - Acta Odontologica Scandinavica
JF - Acta Odontologica Scandinavica
SN - 0001-6357
IS - 3-4
ER -