Plasma cytokines levels in aggressive and chronic periodontitis

Letícia Grando Mattuella; Milene Borges Campagnaro; Andréia Escosteguy Vargas; Léder Leal Xavier; Rui Vicente Oppermann; José Artur Bogo Chies; Letícia Algarves Miranda

doi:10.3109/00016357.2012.715191

Plasma cytokines levels in aggressive and chronic periodontitis

Letícia Grando Mattuella, Milene Borges Campagnaro, Andréia Escosteguy Vargas, Léder Leal Xavier, Rui Vicente Oppermann, José Artur Bogo Chies, Letícia Algarves Miranda

Research output: Contribution to journal › Article

12 Citations (Scopus)

Abstract

OBJECTIVE: The present study evaluated the Th1/Th2 cytokine profile in plasma from healthy controls and different types of periodontitis patients.

MATERIALS AND METHODS: The concentration of IL-2, IL-4, IL-5, IL-10, TNF-α and IFN-γ was determined in healthy controls (n = 18) and patients with chronic (n = 19) and aggressive periodontitis (n = 19) using a flow cytometric multiplex immunoassay. Means and standard deviations were calculated and compared using Kruskal-Wallis test. Spearman rho coefficient was used to correlate cytokines in the studied groups.

RESULTS: Although there was no significant difference in the concentration of cytokines between groups, there was a tendency to lower levels of IL-5 and IL-10 in the aggressive periodontitis group. Stronger correlations were observed between IL-2/IL-4 and IL-2/IL-10 in healthy controls (0.938 and 0.669, respectively) compared with chronic (0.746 and 0.532) and aggressive periodontitis groups (0.395 and 0.266). When compared to healthy (0.812) and chronic periodontitis (0.845) groups, the correlation of IL-4/IL-5 was weaker in the aggressive group (0.459).

CONCLUSION: No difference between systemic levels of Th1/Th2 was observed. In aggressive periodontitis patients, nevertheless, a trend towards low levels of Th2 cytokines could suggest a contribution to the development of such an exacerbated manifestation of this disease.

Original language	English
Pages (from-to)	683-8
Number of pages	6
Journal	Acta Odontologica Scandinavica
Volume	71
Issue number	3-4
DOIs	https://doi.org/10.3109/00016357.2012.715191
Publication status	Published - 5 Sep 2012
Externally published	Yes

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Mattuella, L. G., Campagnaro, M. B., Vargas, A. E., Xavier, L. L., Oppermann, R. V., Chies, J. A. B., & Miranda, L. A. (2012). Plasma cytokines levels in aggressive and chronic periodontitis. Acta Odontologica Scandinavica, 71(3-4), 683-8. https://doi.org/10.3109/00016357.2012.715191

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abstract = "OBJECTIVE: The present study evaluated the Th1/Th2 cytokine profile in plasma from healthy controls and different types of periodontitis patients.MATERIALS AND METHODS: The concentration of IL-2, IL-4, IL-5, IL-10, TNF-α and IFN-γ was determined in healthy controls (n = 18) and patients with chronic (n = 19) and aggressive periodontitis (n = 19) using a flow cytometric multiplex immunoassay. Means and standard deviations were calculated and compared using Kruskal-Wallis test. Spearman rho coefficient was used to correlate cytokines in the studied groups.RESULTS: Although there was no significant difference in the concentration of cytokines between groups, there was a tendency to lower levels of IL-5 and IL-10 in the aggressive periodontitis group. Stronger correlations were observed between IL-2/IL-4 and IL-2/IL-10 in healthy controls (0.938 and 0.669, respectively) compared with chronic (0.746 and 0.532) and aggressive periodontitis groups (0.395 and 0.266). When compared to healthy (0.812) and chronic periodontitis (0.845) groups, the correlation of IL-4/IL-5 was weaker in the aggressive group (0.459).CONCLUSION: No difference between systemic levels of Th1/Th2 was observed. In aggressive periodontitis patients, nevertheless, a trend towards low levels of Th2 cytokines could suggest a contribution to the development of such an exacerbated manifestation of this disease.",

keywords = "Case-Control Studies, Chronic Disease, Cytokines/blood, Female, Humans, Infant, Newborn, Male, Middle Aged, Periodontitis/blood",

author = "Mattuella, {Let{\'i}cia Grando} and Campagnaro, {Milene Borges} and Vargas, {Andr{\'e}ia Escosteguy} and Xavier, {L{\'e}der Leal} and Oppermann, {Rui Vicente} and Chies, {Jos{\'e} Artur Bogo} and Miranda, {Let{\'i}cia Algarves}",

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Plasma cytokines levels in aggressive and chronic periodontitis. / Mattuella, Letícia Grando; Campagnaro, Milene Borges; Vargas, Andréia Escosteguy; Xavier, Léder Leal; Oppermann, Rui Vicente; Chies, José Artur Bogo; Miranda, Letícia Algarves.

In: Acta Odontologica Scandinavica, Vol. 71, No. 3-4, 05.09.2012, p. 683-8.

Research output: Contribution to journal › Article

TY - JOUR

T1 - Plasma cytokines levels in aggressive and chronic periodontitis

AU - Mattuella, Letícia Grando

AU - Campagnaro, Milene Borges

AU - Vargas, Andréia Escosteguy

AU - Xavier, Léder Leal

AU - Oppermann, Rui Vicente

AU - Chies, José Artur Bogo

AU - Miranda, Letícia Algarves

PY - 2012/9/5

Y1 - 2012/9/5

N2 - OBJECTIVE: The present study evaluated the Th1/Th2 cytokine profile in plasma from healthy controls and different types of periodontitis patients.MATERIALS AND METHODS: The concentration of IL-2, IL-4, IL-5, IL-10, TNF-α and IFN-γ was determined in healthy controls (n = 18) and patients with chronic (n = 19) and aggressive periodontitis (n = 19) using a flow cytometric multiplex immunoassay. Means and standard deviations were calculated and compared using Kruskal-Wallis test. Spearman rho coefficient was used to correlate cytokines in the studied groups.RESULTS: Although there was no significant difference in the concentration of cytokines between groups, there was a tendency to lower levels of IL-5 and IL-10 in the aggressive periodontitis group. Stronger correlations were observed between IL-2/IL-4 and IL-2/IL-10 in healthy controls (0.938 and 0.669, respectively) compared with chronic (0.746 and 0.532) and aggressive periodontitis groups (0.395 and 0.266). When compared to healthy (0.812) and chronic periodontitis (0.845) groups, the correlation of IL-4/IL-5 was weaker in the aggressive group (0.459).CONCLUSION: No difference between systemic levels of Th1/Th2 was observed. In aggressive periodontitis patients, nevertheless, a trend towards low levels of Th2 cytokines could suggest a contribution to the development of such an exacerbated manifestation of this disease.

AB - OBJECTIVE: The present study evaluated the Th1/Th2 cytokine profile in plasma from healthy controls and different types of periodontitis patients.MATERIALS AND METHODS: The concentration of IL-2, IL-4, IL-5, IL-10, TNF-α and IFN-γ was determined in healthy controls (n = 18) and patients with chronic (n = 19) and aggressive periodontitis (n = 19) using a flow cytometric multiplex immunoassay. Means and standard deviations were calculated and compared using Kruskal-Wallis test. Spearman rho coefficient was used to correlate cytokines in the studied groups.RESULTS: Although there was no significant difference in the concentration of cytokines between groups, there was a tendency to lower levels of IL-5 and IL-10 in the aggressive periodontitis group. Stronger correlations were observed between IL-2/IL-4 and IL-2/IL-10 in healthy controls (0.938 and 0.669, respectively) compared with chronic (0.746 and 0.532) and aggressive periodontitis groups (0.395 and 0.266). When compared to healthy (0.812) and chronic periodontitis (0.845) groups, the correlation of IL-4/IL-5 was weaker in the aggressive group (0.459).CONCLUSION: No difference between systemic levels of Th1/Th2 was observed. In aggressive periodontitis patients, nevertheless, a trend towards low levels of Th2 cytokines could suggest a contribution to the development of such an exacerbated manifestation of this disease.

KW - Case-Control Studies

KW - Chronic Disease

KW - Cytokines/blood

KW - Female

KW - Humans

KW - Infant, Newborn

KW - Male

KW - Middle Aged

KW - Periodontitis/blood

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DO - 10.3109/00016357.2012.715191

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SP - 683

EP - 688

JO - Acta Odontologica Scandinavica

JF - Acta Odontologica Scandinavica

SN - 0001-6357

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ER -

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10.3109/00016357.2012.715191