Abstract
| Original language | English |
|---|---|
| Pages (from-to) | E122-E126 |
| Journal | Journal of Clinical Endocrinology and Metabolism |
| Volume | 99 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 2014 |
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Plasma apolipoprotein B-48 transport in obese men: A new tracer kinetic study in the postprandial state. / Wong, A.T.Y.; Chan, Dick; Pang, Jing; Watts, Gerald; Barrett, Hugh.
In: Journal of Clinical Endocrinology and Metabolism, Vol. 99, No. 1, 01.2014, p. E122-E126.Research output: Contribution to journal › Article
TY - JOUR
T1 - Plasma apolipoprotein B-48 transport in obese men: A new tracer kinetic study in the postprandial state
AU - Wong, A.T.Y.
AU - Chan, Dick
AU - Pang, Jing
AU - Watts, Gerald
AU - Barrett, Hugh
PY - 2014/1
Y1 - 2014/1
N2 - Context: The mechanisms responsible for impaired chylomicron metabolism have not been adequately investigated in obese subjects. Objective: We aimed to compare apolipoprotein (apo) B-48 kinetics in obese and lean men by developing a new model to describe the kinetics of apoB-48 particles in the postprandial state. Design, Setting, and Patients: Seven obese and 13 age-matched lean men were given an oral fat load. apoB-48 tracer to tracee ratios were measured after intravenous d3-leucine administration using gas chromatography-mass spectrometry. Kinetic parameters were derived using a multicompartmental model. Outcomes Measures: Plasma total and incremental apoB-48 0-10 hour areas under the curve as well as apoB-48 secretion and fractional catabolic rate. Results: Compared with lean men, fasting plasma triglyceride (+148%) and apoB-48 (+110%) concentrations as well as plasma total and incremental triglycerides (+184% and +185%, respectively) and apoB-48 (+182% and 224%, respectively) areas under the curve were significantly higher in obese men (P <.05 for all). The obese men also had significantly (P <.05 for all) higher secretion rates of apoB-48 in the fasted state (+145%)as well as at 3 hours (+70%), 4 hours (+82%), 5 hours (+82%), 6 hours (+76%), and 8 hours (+61%) in response to the fat load. This was associated with a greater number of apoB-48-containing particles secreted over the 10-hour study period in the obese men, compared with lean men (+125%, P <.01). The fractional catabolic rate of apoB-48 was significantly lower in the obese men compared with the lean men (-33%, P <.05) Conclusion: We demonstrate that postprandial hypertriglyceridemia in central obesity relates to an overproduction and impaired catabolism of apoB-48-containing lipoproteins. These findings are based on a new, physiologically relevant, kinetic model ,which describes the non-steady-state postprandial metabolism of apoB-48. (J Clin Endocrinol Metab 99: E122-E126, 2014) © Copyright 2014 by The Endocrine Society.
AB - Context: The mechanisms responsible for impaired chylomicron metabolism have not been adequately investigated in obese subjects. Objective: We aimed to compare apolipoprotein (apo) B-48 kinetics in obese and lean men by developing a new model to describe the kinetics of apoB-48 particles in the postprandial state. Design, Setting, and Patients: Seven obese and 13 age-matched lean men were given an oral fat load. apoB-48 tracer to tracee ratios were measured after intravenous d3-leucine administration using gas chromatography-mass spectrometry. Kinetic parameters were derived using a multicompartmental model. Outcomes Measures: Plasma total and incremental apoB-48 0-10 hour areas under the curve as well as apoB-48 secretion and fractional catabolic rate. Results: Compared with lean men, fasting plasma triglyceride (+148%) and apoB-48 (+110%) concentrations as well as plasma total and incremental triglycerides (+184% and +185%, respectively) and apoB-48 (+182% and 224%, respectively) areas under the curve were significantly higher in obese men (P <.05 for all). The obese men also had significantly (P <.05 for all) higher secretion rates of apoB-48 in the fasted state (+145%)as well as at 3 hours (+70%), 4 hours (+82%), 5 hours (+82%), 6 hours (+76%), and 8 hours (+61%) in response to the fat load. This was associated with a greater number of apoB-48-containing particles secreted over the 10-hour study period in the obese men, compared with lean men (+125%, P <.01). The fractional catabolic rate of apoB-48 was significantly lower in the obese men compared with the lean men (-33%, P <.05) Conclusion: We demonstrate that postprandial hypertriglyceridemia in central obesity relates to an overproduction and impaired catabolism of apoB-48-containing lipoproteins. These findings are based on a new, physiologically relevant, kinetic model ,which describes the non-steady-state postprandial metabolism of apoB-48. (J Clin Endocrinol Metab 99: E122-E126, 2014) © Copyright 2014 by The Endocrine Society.
U2 - 10.1210/jc.2013-2477
DO - 10.1210/jc.2013-2477
M3 - Article
VL - 99
SP - E122-E126
JO - Journal of Endocrinology & Metabolism
JF - Journal of Endocrinology & Metabolism
SN - 0021-972X
IS - 1
ER -