Plants in constrained canopy micro-swards compensate for decreased root biomass and soil exploration with increased amounts of rhizosphere carboxylates

Robert P. Jeffery, Richard J. Simpson, Hans Lambers, Daniel R. Kidd, Megan H. Ryan

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3 Citations (Scopus)
133 Downloads (Pure)

Abstract

Root traits related to phosphorus (P) acquisition are used to make inferences about a species' P-foraging ability under glasshouse conditions. However, the effect on such root traits of constrained canopy spread, as occurs in dense pasture swards, is unknown. We grew micro-swards of Trifolium subterraneum L. and Ornithopus compressus L. at 15 and 60mg kg-1 soil P in a glasshouse. Shoots either spread beyond the pot perimeter or were constrained by a cylindrical sleeve adjusted to canopy height. After 8 weeks, shoot and root dry mass (DM), shoot tissue P concentration, rhizosphere carboxylates, arbuscular mycorrhizal (AM) fungal colonisation, total and specific root length (TRL and SRL respectively), average root diameter (ARD) and average root hair length (ARHL) were measured. In all species and treatments, constrained canopy spread decreased root DM (39-59%), TRL (27-45%) and shoot DM (10-28%), and increased SRL (20-33%), but did not affect ARD, ARHL and AM fungal colonisation. However, shoot P concentration and content increased, and rhizosphere carboxylates increased 3.5 to 12-fold per unit RL and 2.0- to 6.5-fold per micro-sward. Greater amounts of rhizosphere carboxylates when canopy spread was constrained appeared to compensate for reduced root growth enabling shoot P content to be maintained.

Original languageEnglish
Pages (from-to)552-562
Number of pages11
JournalFunctional Plant Biology
Volume44
Issue number5
DOIs
Publication statusPublished - 2017

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sward
rhizosphere
canopy
biomass
shoots
soil
root hairs
Ornithopus compressus
greenhouses
Trifolium subterraneum
root growth
pastures
foraging
phosphorus

Cite this

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title = "Plants in constrained canopy micro-swards compensate for decreased root biomass and soil exploration with increased amounts of rhizosphere carboxylates",
abstract = "Root traits related to phosphorus (P) acquisition are used to make inferences about a species' P-foraging ability under glasshouse conditions. However, the effect on such root traits of constrained canopy spread, as occurs in dense pasture swards, is unknown. We grew micro-swards of Trifolium subterraneum L. and Ornithopus compressus L. at 15 and 60mg kg-1 soil P in a glasshouse. Shoots either spread beyond the pot perimeter or were constrained by a cylindrical sleeve adjusted to canopy height. After 8 weeks, shoot and root dry mass (DM), shoot tissue P concentration, rhizosphere carboxylates, arbuscular mycorrhizal (AM) fungal colonisation, total and specific root length (TRL and SRL respectively), average root diameter (ARD) and average root hair length (ARHL) were measured. In all species and treatments, constrained canopy spread decreased root DM (39-59{\%}), TRL (27-45{\%}) and shoot DM (10-28{\%}), and increased SRL (20-33{\%}), but did not affect ARD, ARHL and AM fungal colonisation. However, shoot P concentration and content increased, and rhizosphere carboxylates increased 3.5 to 12-fold per unit RL and 2.0- to 6.5-fold per micro-sward. Greater amounts of rhizosphere carboxylates when canopy spread was constrained appeared to compensate for reduced root growth enabling shoot P content to be maintained.",
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Plants in constrained canopy micro-swards compensate for decreased root biomass and soil exploration with increased amounts of rhizosphere carboxylates. / Jeffery, Robert P.; Simpson, Richard J.; Lambers, Hans; Kidd, Daniel R.; Ryan, Megan H.

In: Functional Plant Biology, Vol. 44, No. 5, 2017, p. 552-562.

Research output: Contribution to journalArticle

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AU - Ryan, Megan H.

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