Abstract
Recalcitrant regeneration is one of the bottlenecks for plant
improvement, especially in monocotyledonous species. In this
research, plantlet regeneration of a native rice cultivar Oryza sativa ‘Barak Cenana’, high-value local Balinese red rice was successfully demonstrated. Compact nodular calli were induced from excised seedderived mature embryos of ‘Barak Cenana’ rice. Although 2,4-D was necessary for callus induction, a combination of 2,4-D in regeneration medium caused reduction of green spot and shoot meristemoid differentiation. Root differentiation was firstly regenerated where meristematic shoots were thereafter developed from calli. The highest number of shoot regeneration regenerated from green spots and numbers of plantlet with complete shoot/root development were observed when transferred calli to Murashige and Skoog (MS) medium
containing 5 mgL-1. Multiple shoot formation of regenerated plantlets revealed for efficient regeneration system, which was firstly reported in this present study and will potentially serve for plant improvement of this native rice, Barak Cenana.
improvement, especially in monocotyledonous species. In this
research, plantlet regeneration of a native rice cultivar Oryza sativa ‘Barak Cenana’, high-value local Balinese red rice was successfully demonstrated. Compact nodular calli were induced from excised seedderived mature embryos of ‘Barak Cenana’ rice. Although 2,4-D was necessary for callus induction, a combination of 2,4-D in regeneration medium caused reduction of green spot and shoot meristemoid differentiation. Root differentiation was firstly regenerated where meristematic shoots were thereafter developed from calli. The highest number of shoot regeneration regenerated from green spots and numbers of plantlet with complete shoot/root development were observed when transferred calli to Murashige and Skoog (MS) medium
containing 5 mgL-1. Multiple shoot formation of regenerated plantlets revealed for efficient regeneration system, which was firstly reported in this present study and will potentially serve for plant improvement of this native rice, Barak Cenana.
| Original language | English |
|---|---|
| Pages (from-to) | S12 -S 17 |
| Number of pages | 6 |
| Journal | Bali Medical Journal |
| Volume | 3 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 17 Jul 2017 |
| Externally published | Yes |
| Event | Integrated Biomedical Laboratory Conference 2017 - Duration: 1 Jan 2017 → … |