Phyto-oestrogens affect in vitro fertilization and embryo development in sheep

Anna Amir, Jennifer Kelly, David Kleemann, Zoey Durmic, Dominique Blache, Graeme Martin

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Phyto-oestrogens such as isoflavones are natural compounds that can profoundly affect reproductive function. We tested whether including isoflavone compounds (genistein, biochanin A, formononetin) in the maturation medium would affect the outcomes for ovine oocytes in vitro. A factorial design (3 isoflavones x 5 concentrations: 0, 2.5, 5, 10, 25 µg mL-1) was used and the entire protocol was repeated four times. Cumulus-oocyte-complexes were randomly allocated to the treatments, then fertilized and cultured in vitro. Compared with control (0 µg mL-1), the lower concentrations of isoflavone (2.5, 5 and 10 µg mL-1) had no detectable effect on the rates of cleavage or embryo development, or on embryo total cell counts (TCC). However, the highest concentration (25 µg mL-1) of all three isoflavones exerted a variety of effects (p < 0.05): genistein decreased cleavage rate, blastocyst rate and blastocyst efficiency (blastocysts produced per 100 oocytes); biochanin A decreased cleavage rate and blastocyst efficiency; and formononetin decreased blastocyst rate and blastocyst efficiency. Biochanin A (25 µg mL-1) reduced embryo TCC specifically at the hatched blastocyst stage (P < 0.05). We conclude that the presence of isoflavones at 25 µg mL-1 during in vitro maturation decreases the cleavage rate and inhibits blastocyst hatching.
Original languageEnglish
Pages (from-to)1109-1115
Number of pages16
JournalReproduction, Fertility and Development
Volume30
Issue number8
DOIs
Publication statusPublished - 2018

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Phytoestrogens
in vitro fertilization
Blastocyst
Fertilization in Vitro
blastocyst
estrogens
Isoflavones
Embryonic Development
Sheep
embryogenesis
isoflavones
sheep
biochanin A
formononetin
Oocytes
oocytes
Genistein
genistein
embryo (animal)
Embryonic Structures

Cite this

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title = "Phyto-oestrogens affect in vitro fertilization and embryo development in sheep",
abstract = "Phyto-oestrogens such as isoflavones are natural compounds that can profoundly affect reproductive function. We tested whether including isoflavone compounds (genistein, biochanin A, formononetin) in the maturation medium would affect the outcomes for ovine oocytes in vitro. A factorial design (3 isoflavones x 5 concentrations: 0, 2.5, 5, 10, 25 µg mL-1) was used and the entire protocol was repeated four times. Cumulus-oocyte-complexes were randomly allocated to the treatments, then fertilized and cultured in vitro. Compared with control (0 µg mL-1), the lower concentrations of isoflavone (2.5, 5 and 10 µg mL-1) had no detectable effect on the rates of cleavage or embryo development, or on embryo total cell counts (TCC). However, the highest concentration (25 µg mL-1) of all three isoflavones exerted a variety of effects (p < 0.05): genistein decreased cleavage rate, blastocyst rate and blastocyst efficiency (blastocysts produced per 100 oocytes); biochanin A decreased cleavage rate and blastocyst efficiency; and formononetin decreased blastocyst rate and blastocyst efficiency. Biochanin A (25 µg mL-1) reduced embryo TCC specifically at the hatched blastocyst stage (P < 0.05). We conclude that the presence of isoflavones at 25 µg mL-1 during in vitro maturation decreases the cleavage rate and inhibits blastocyst hatching.",
author = "Anna Amir and Jennifer Kelly and David Kleemann and Zoey Durmic and Dominique Blache and Graeme Martin",
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Phyto-oestrogens affect in vitro fertilization and embryo development in sheep. / Amir, Anna; Kelly, Jennifer; Kleemann, David; Durmic, Zoey; Blache, Dominique; Martin, Graeme.

In: Reproduction, Fertility and Development, Vol. 30, No. 8, 2018, p. 1109-1115.

Research output: Contribution to journalArticle

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AU - Kelly, Jennifer

AU - Kleemann, David

AU - Durmic, Zoey

AU - Blache, Dominique

AU - Martin, Graeme

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AB - Phyto-oestrogens such as isoflavones are natural compounds that can profoundly affect reproductive function. We tested whether including isoflavone compounds (genistein, biochanin A, formononetin) in the maturation medium would affect the outcomes for ovine oocytes in vitro. A factorial design (3 isoflavones x 5 concentrations: 0, 2.5, 5, 10, 25 µg mL-1) was used and the entire protocol was repeated four times. Cumulus-oocyte-complexes were randomly allocated to the treatments, then fertilized and cultured in vitro. Compared with control (0 µg mL-1), the lower concentrations of isoflavone (2.5, 5 and 10 µg mL-1) had no detectable effect on the rates of cleavage or embryo development, or on embryo total cell counts (TCC). However, the highest concentration (25 µg mL-1) of all three isoflavones exerted a variety of effects (p < 0.05): genistein decreased cleavage rate, blastocyst rate and blastocyst efficiency (blastocysts produced per 100 oocytes); biochanin A decreased cleavage rate and blastocyst efficiency; and formononetin decreased blastocyst rate and blastocyst efficiency. Biochanin A (25 µg mL-1) reduced embryo TCC specifically at the hatched blastocyst stage (P < 0.05). We conclude that the presence of isoflavones at 25 µg mL-1 during in vitro maturation decreases the cleavage rate and inhibits blastocyst hatching.

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