Physiological changes in white lupin associated with variation in root-zone CO2 concentration and cluster-root P mobilization

Michael Cramer, Michael Shane, Hans Lambers

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

White lupin (Lupinus albus L.) mobilizes insoluble soil phosphorus through exudation of organic acids from 'cluster' roots. Organic acid synthesis requires anaplerotic carbon derived from dark CO2 fixation involving PEP-carboxylase. We tested the hypothesis that variation in root-zone CO2 concentration would influence organic acid synthesis and thus P mobilization. Root-zone CO2 concentrations and soil FePO4 concentrations supplied to sand-grown white lupin (cv. Kiev Mutant) were varied. More biomass accumulated in plants supplied with 360 mu L L-1 CO2 to the root-zone, compared with those aerated with either 100 or 6000 mu L L-1 CO2. Increased FePO4 in the sand resulted in greater leaf P concentrations, but root-zone [CO2] did not influence leaf P concentration. Suppression of cluster-root development in plants supplied with 100 mu L L-1 root-zone CO2 was correlated with increased leaf [P]. However, at both 360 and 6000 mu L L-1 CO2, cluster-root development was suppressed only at the highest leaf P concentration. Phloem sap [P] was significantly increased by greater [FePO4] in the sand, but was reduced with increased root-zone [CO2], and this may have triggered increased cluster-root initiation. Succinate was the major organic acid (carboxylate) in the phloem sap (minor components included malate, citrate, fumarate) and was increased at greater [FePO4], suggesting that this shoot-derived carboxylate might provide an important source of organic acids for root metabolism. Since cluster root development was inhibited by increasing concentrations of FePO4 in the sand, it is possible that succinate was utilized for the functioning of the root-nodules.
Original languageEnglish
Pages (from-to)1203-1217
JournalPlant, Cell and Environment
Volume28
Issue number10
DOIs
Publication statusPublished - 2005

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Lupinus
rhizosphere
organic acids and salts
Acids
Phloem
Synthetic Chemistry Techniques
sand
Succinic Acid
succinic acid
sap
Soil
phloem
leaves
Fumarates
Plant Development
Lupinus albus
synthesis
Citric Acid
Biomass
Phosphorus

Cite this

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title = "Physiological changes in white lupin associated with variation in root-zone CO2 concentration and cluster-root P mobilization",
abstract = "White lupin (Lupinus albus L.) mobilizes insoluble soil phosphorus through exudation of organic acids from 'cluster' roots. Organic acid synthesis requires anaplerotic carbon derived from dark CO2 fixation involving PEP-carboxylase. We tested the hypothesis that variation in root-zone CO2 concentration would influence organic acid synthesis and thus P mobilization. Root-zone CO2 concentrations and soil FePO4 concentrations supplied to sand-grown white lupin (cv. Kiev Mutant) were varied. More biomass accumulated in plants supplied with 360 mu L L-1 CO2 to the root-zone, compared with those aerated with either 100 or 6000 mu L L-1 CO2. Increased FePO4 in the sand resulted in greater leaf P concentrations, but root-zone [CO2] did not influence leaf P concentration. Suppression of cluster-root development in plants supplied with 100 mu L L-1 root-zone CO2 was correlated with increased leaf [P]. However, at both 360 and 6000 mu L L-1 CO2, cluster-root development was suppressed only at the highest leaf P concentration. Phloem sap [P] was significantly increased by greater [FePO4] in the sand, but was reduced with increased root-zone [CO2], and this may have triggered increased cluster-root initiation. Succinate was the major organic acid (carboxylate) in the phloem sap (minor components included malate, citrate, fumarate) and was increased at greater [FePO4], suggesting that this shoot-derived carboxylate might provide an important source of organic acids for root metabolism. Since cluster root development was inhibited by increasing concentrations of FePO4 in the sand, it is possible that succinate was utilized for the functioning of the root-nodules.",
author = "Michael Cramer and Michael Shane and Hans Lambers",
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volume = "28",
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Physiological changes in white lupin associated with variation in root-zone CO2 concentration and cluster-root P mobilization. / Cramer, Michael; Shane, Michael; Lambers, Hans.

In: Plant, Cell and Environment, Vol. 28, No. 10, 2005, p. 1203-1217.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Physiological changes in white lupin associated with variation in root-zone CO2 concentration and cluster-root P mobilization

AU - Cramer, Michael

AU - Shane, Michael

AU - Lambers, Hans

PY - 2005

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N2 - White lupin (Lupinus albus L.) mobilizes insoluble soil phosphorus through exudation of organic acids from 'cluster' roots. Organic acid synthesis requires anaplerotic carbon derived from dark CO2 fixation involving PEP-carboxylase. We tested the hypothesis that variation in root-zone CO2 concentration would influence organic acid synthesis and thus P mobilization. Root-zone CO2 concentrations and soil FePO4 concentrations supplied to sand-grown white lupin (cv. Kiev Mutant) were varied. More biomass accumulated in plants supplied with 360 mu L L-1 CO2 to the root-zone, compared with those aerated with either 100 or 6000 mu L L-1 CO2. Increased FePO4 in the sand resulted in greater leaf P concentrations, but root-zone [CO2] did not influence leaf P concentration. Suppression of cluster-root development in plants supplied with 100 mu L L-1 root-zone CO2 was correlated with increased leaf [P]. However, at both 360 and 6000 mu L L-1 CO2, cluster-root development was suppressed only at the highest leaf P concentration. Phloem sap [P] was significantly increased by greater [FePO4] in the sand, but was reduced with increased root-zone [CO2], and this may have triggered increased cluster-root initiation. Succinate was the major organic acid (carboxylate) in the phloem sap (minor components included malate, citrate, fumarate) and was increased at greater [FePO4], suggesting that this shoot-derived carboxylate might provide an important source of organic acids for root metabolism. Since cluster root development was inhibited by increasing concentrations of FePO4 in the sand, it is possible that succinate was utilized for the functioning of the root-nodules.

AB - White lupin (Lupinus albus L.) mobilizes insoluble soil phosphorus through exudation of organic acids from 'cluster' roots. Organic acid synthesis requires anaplerotic carbon derived from dark CO2 fixation involving PEP-carboxylase. We tested the hypothesis that variation in root-zone CO2 concentration would influence organic acid synthesis and thus P mobilization. Root-zone CO2 concentrations and soil FePO4 concentrations supplied to sand-grown white lupin (cv. Kiev Mutant) were varied. More biomass accumulated in plants supplied with 360 mu L L-1 CO2 to the root-zone, compared with those aerated with either 100 or 6000 mu L L-1 CO2. Increased FePO4 in the sand resulted in greater leaf P concentrations, but root-zone [CO2] did not influence leaf P concentration. Suppression of cluster-root development in plants supplied with 100 mu L L-1 root-zone CO2 was correlated with increased leaf [P]. However, at both 360 and 6000 mu L L-1 CO2, cluster-root development was suppressed only at the highest leaf P concentration. Phloem sap [P] was significantly increased by greater [FePO4] in the sand, but was reduced with increased root-zone [CO2], and this may have triggered increased cluster-root initiation. Succinate was the major organic acid (carboxylate) in the phloem sap (minor components included malate, citrate, fumarate) and was increased at greater [FePO4], suggesting that this shoot-derived carboxylate might provide an important source of organic acids for root metabolism. Since cluster root development was inhibited by increasing concentrations of FePO4 in the sand, it is possible that succinate was utilized for the functioning of the root-nodules.

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DO - 10.1111/j.1365-3040.2005.01358.x

M3 - Article

VL - 28

SP - 1203

EP - 1217

JO - Plant, Cell and Environment.

JF - Plant, Cell and Environment.

SN - 0140-7791

IS - 10

ER -