Photon counting imaging with an electron-bombarded CCD: Towards a parallel-processing photoelectronic time-to-amplitude converter

Liisa M. Hirvonen, Stephen Jiggins, Nicolas Sergent, Gianmarco Zanda, Klaus Suhling

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

We have used an electron-bombarded CCD for optical photon counting imaging. The photon event pulse height distribution was found to be linearly dependent on the gain voltage. We propose on this basis that a gain voltage sweep during exposure in an electron-bombarded sensor would allow photon arrival time determination with sub-frame exposure time resolution. This effectively uses an electron-bombarded sensor as a parallel-processing photoelectronic time-to-amplitude converter, or a two-dimensional photon counting streak camera. Several applications that require timing of photon arrival, including Fluorescence Lifetime Imaging Microscopy, may benefit from such an approach. A simulation of a voltage sweep performed with experimental data collected with different acceleration voltages validates the principle of this approach. Moreover, photon event centroiding was performed and a hybrid 50% Gaussian/Centre of Gravity + 50% Hyperbolic cosine centroiding algorithm was found to yield the lowest fixed pattern noise. Finally, the camera was mounted on a fluorescence microscope to image F-actin filaments stained with the fluorescent dye Alexa 488 in fixed cells.

Original languageEnglish
Article number123102
JournalReview of Scientific Instruments
Volume85
Issue number12
DOIs
Publication statusPublished - 1 Dec 2014
Externally publishedYes

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