Pheophorbide A may regulate jasmonate signaling during DARK-INDUCED SENESCENCE

Sylvain Aubry, Niklaus Fankhauser, Serguei Ovinnikov, Adriana Pruzinská, Marina Stirnemann, Krzysztof Zienkiewicz, Cornelia Herrfurth, Ivo Feussner, Stefan Hörtensteiner

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Abstract

Chlorophyll degradation is one of the most visible signs of leaf senescence. During senescence, chlorophyll is degraded in the multistep pheophorbide a oxygenase (PAO)/phyllobilin pathway. This pathway is tightly regulated at the transcriptional level, allowing coordinated and efficient remobilization of nitrogen toward sink organs. Using a combination of transcriptome and metabolite analyses during dark-induced senescence of Arabidopsis (Arabidopsis thaliana) mutants deficient in key steps of the PAO/phyllobilin pathway, we show an unanticipated role for one of the pathway intermediates, i.e. pheophorbide a. Both jasmonic acid-related gene expression and jasmonic acid precursors specifically accumulated in pao1, a mutant deficient in PAO. We propose that pheophorbide a, the last intact porphyrin intermediate of chlorophyll degradation and a unique pathway “bottleneck,” has been recruited as a signaling molecule of chloroplast metabolic status. Our work challenges the assumption that chlorophyll breakdown is merely a result of senescence, and proposes that the flux of pheophorbide a through the pathway acts in a feed-forward loop that remodels the nuclear transcriptome and controls the pace of chlorophyll degradation in senescing leaves.

Original languageEnglish
Pages (from-to)776-791
Number of pages16
JournalPlant Physiology
Volume182
Issue number2
DOIs
Publication statusPublished - Feb 2020

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    Aubry, S., Fankhauser, N., Ovinnikov, S., Pruzinská, A., Stirnemann, M., Zienkiewicz, K., Herrfurth, C., Feussner, I., & Hörtensteiner, S. (2020). Pheophorbide A may regulate jasmonate signaling during DARK-INDUCED SENESCENCE. Plant Physiology, 182(2), 776-791. https://doi.org/10.1104/pp.19.01115