Phenol coupling in fungal natural product biosynthesis

Jinyu Hu

Research output: ThesisDoctoral Thesis

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Abstract

Fungi produce myriad biaryl natural products that exhibit various biological activities. How fungi control the regio- and stereo-selectivity in the coupling of two aryl units to make such biaryl compounds was not fully understood. By investigating the biosynthesis of two axial chiral biaryls, namely elsinochrome C and (-)-viriditoxin, the work in this thesis demonstrate that fungal multicopper oxidases are able to control the regioselectivity of the coupling reactions, while requiring accessory proteins to mediate the coupling stereo-selectivity. A heterologous expression system was constructed to facilitate the biosynthetic studies. The study opens up avenues to synthesise biaryl molecules for drug discovery.
Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • The University of Western Australia
Supervisors/Advisors
  • Chooi, Heng, Supervisor
  • Stubbs, Keith, Supervisor
Thesis sponsors
Award date15 Apr 2020
DOIs
Publication statusUnpublished - 2020

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