Abstract
Fungi produce myriad biaryl natural products that exhibit various biological activities. How fungi control the regio- and stereo-selectivity in the coupling of two aryl units to make such biaryl compounds was not fully understood. By investigating the biosynthesis of two axial chiral biaryls, namely elsinochrome C and (-)-viriditoxin, the work in this thesis demonstrate that fungal multicopper oxidases are able to control the regioselectivity of the coupling reactions, while requiring accessory proteins to mediate the coupling stereo-selectivity. A heterologous expression system was constructed to facilitate the biosynthetic studies. The study opens up avenues to synthesise biaryl molecules for drug discovery.
Original language | English |
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Qualification | Doctor of Philosophy |
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Award date | 15 Apr 2020 |
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Publication status | Unpublished - 2020 |