Pharmacological activity of peroral chitosan-insulin nanoparticles in diabetic rats

Z. Ma, T.M. Lim, Lee Yong Lim

    Research output: Contribution to journalArticle

    199 Citations (Scopus)

    Abstract

    The objective of the present study was to evaluate the effects of formulation parameters on the in vivo pharmacological activity of the chitosan-insulin nanoparticles. Chitosan-insulin nanoparticles were prepared by ionotropic gelation at pH 5.3 and 6.1 and denoted as F5.3 np and F6.1 np, respectively. F5.3np and F6.1 np administered orally at insulin doses of 50 U/kg and/or 100 U/kg were effective at lowering the serum glucose level of streptozotocin-induced diabetic rats. The 100 U/kg-dose F5.3 np sustained the serum glucose at pre-diabetic levels for at least 11h. In comparison, F6.1 np, had a faster onset of action (2 h versus 10 h) but lower efficiency. The effectiveness of peroral F5.3 np and F6.1 np in lowering the serum glucose level of streptozotocin-induced diabetic rats was ascribed to the local effect of insulin in intestine. Confocal micrographs showed strong interaction between rat intestinal epithelium and chitosan nanoparticles 3 h post-oral administration. (c) 2005 Elsevier B.V. All rights reserved.
    Original languageEnglish
    Pages (from-to)271-280
    JournalInternational Journal of Pharmaceutics
    Volume293
    Issue number1-2
    DOIs
    Publication statusPublished - 2005

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