Pharmaceutical characterization of probucol bile acid-lithocholic acid nanoparticles to prevent chronic hearing related and similar cellular oxidative stress pathologies

Susbin R. Wagle, Corina M. Ionescu, Bozica Kovacevic, Melissa Jones, Thomas Foster, Patrick Lim, Michael Lewkowicz, Maja Ðani´c, Momir Mikov, Armin Mooranian, Hani Al-Salami

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


Background: Sensorineural hearing loss has been associated with oxidative stress. However, an antioxidant that passes effectively through the ear remains elusive. Method: Probucol (PB)-based nanoparticles were formed using a spray-drying encapsulation technique, characterized and tested in vitro. Results: Uniform, spherical nanoparticles were produced. The addition of lithocholic acid to PB formulations did not affect drug content or production yield, but it did modify capsule size, surface tension, electrokinetic stability and drug release. Cell viability, bioenergetics and inflammatory profiles were improved when auditory cells were exposed to PB-based nanoparticles, which showed antioxidant properties (p < 0.05). Conclusion: PB-based nanoparticles can potentially protect the auditory cell line from oxidative stress and could be used in future in vivo studies as a potential new therapeutic agent for sensorineural hearing loss.

Original languageEnglish
Pages (from-to)923-940
Number of pages18
Issue number12
Publication statusPublished - 1 May 2023

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