Phage-Type RNA Polymerase RPOTmp Performs Gene-Specific Transcription in Mitochondria of Arabidopsis thaliana

Kristina Kuehn, U. Richter, Etienne Meyer, Etienne Delannoy, Andeol Falcon De Longevialle, Nicholas O'Toole, T. Borner, Harvey Millar, Ian Small, James Whelan

Research output: Contribution to journalArticle

99 Citations (Scopus)

Abstract

Transcription of mitochondrial genes in animals, fungi, and plants relies on the activity of T3/T7 phage-type RNApolymerases. Two such enzymes, RPOTm and RPOTmp, are present in the mitochondria of eudicotyledonous plants;RPOTmp is additionally found in plastids. We have characterized the transcriptional role of the dual-targeted RNApolymerase in mitochondria of Arabidopsis thaliana. Examination of mitochondrial transcripts in rpoTmp mutants revealedmajor differences in transcript abundances between wild-type and rpoTmp plants. Decreased levels of specific transcriptswere correlated with reduced abundances of the respiratory chain complexes I and IV. Altered transcript levels in rpoTmpwere found to result from gene-specific transcriptional changes, establishing that RPOTmp functions in distinct transcriptionalprocesses within mitochondria. Decreased transcription of specific genes in rpoTmp was not associated withchanges in promoter utilization; therefore, RPOTmp function is not promoter specific but gene specific. This implies thatadditional gene-specific elements direct the transcription of a subset of mitochondrial genes by RPOTmp.
Original languageEnglish
Pages (from-to)2762-2779
JournalThe Plant Cell
Volume21
Issue number9
DOIs
Publication statusPublished - 2009

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