Permeability of a growing biofilm in a porous media fluid flow analyzed by magnetic resonance displacement-relaxation correlations

Sarah Vogt, A.B. Sanderlin, J.D. Seymour, S.L. Codd

    Research output: Contribution to journalArticle

    21 Citations (Scopus)

    Abstract

    Biofilm growth in porous media is difficult to study non-invasively due to the opaqueness and heterogeneity of the systems. Magnetic resonance is utilized to non-invasively study water dynamics within porous media. Displacement-relaxation correlation experiments were performed on fluid flow during biofilm growth in a model porous media of mono-dispersed polystyrene beads. The spin-spin T2 magnetic relaxation distinguishes between the biofilm phase and bulk fluid phase due to water-biopolymer interactions present in the biofilm, and the flow dynamics are measured using PGSE NMR experiments. By correlating these two measurements, the effects of biofilm growth on the fluid dynamics can be separated into a detailed analysis of both the biofilm phase and the fluid phase simultaneously within the same experiment. Within the displacement resolution of these experiments, no convective flow was measured through the biomass. An increased amount of longitudinal hydrodynamic dispersion indicates increased hydrodynamic mixing due to fluid channeling caused by biofilm growth. The effect of different biofilm growth conditions was measured by varying the strength of the bacterial growth medium. Biotechnol. Bioeng. 2013; 110: 1366-1375. © 2012 Wiley Periodicals, Inc.
    Original languageEnglish
    Pages (from-to)1366-1375
    JournalBiotechnology and Bioengineering
    Volume110
    Issue number5
    DOIs
    Publication statusPublished - 2013

    Fingerprint

    Biofilms
    Magnetic resonance
    Porous materials
    Flow of fluids
    Permeability
    Magnetic Resonance Spectroscopy
    Hydrodynamics
    Growth
    Fluids
    Experiments
    Magnetic relaxation
    Biopolymers
    Water
    Polystyrenes
    Fluid dynamics
    Biomass
    Nuclear magnetic resonance

    Cite this

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    title = "Permeability of a growing biofilm in a porous media fluid flow analyzed by magnetic resonance displacement-relaxation correlations",
    abstract = "Biofilm growth in porous media is difficult to study non-invasively due to the opaqueness and heterogeneity of the systems. Magnetic resonance is utilized to non-invasively study water dynamics within porous media. Displacement-relaxation correlation experiments were performed on fluid flow during biofilm growth in a model porous media of mono-dispersed polystyrene beads. The spin-spin T2 magnetic relaxation distinguishes between the biofilm phase and bulk fluid phase due to water-biopolymer interactions present in the biofilm, and the flow dynamics are measured using PGSE NMR experiments. By correlating these two measurements, the effects of biofilm growth on the fluid dynamics can be separated into a detailed analysis of both the biofilm phase and the fluid phase simultaneously within the same experiment. Within the displacement resolution of these experiments, no convective flow was measured through the biomass. An increased amount of longitudinal hydrodynamic dispersion indicates increased hydrodynamic mixing due to fluid channeling caused by biofilm growth. The effect of different biofilm growth conditions was measured by varying the strength of the bacterial growth medium. Biotechnol. Bioeng. 2013; 110: 1366-1375. {\circledC} 2012 Wiley Periodicals, Inc.",
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    Permeability of a growing biofilm in a porous media fluid flow analyzed by magnetic resonance displacement-relaxation correlations. / Vogt, Sarah; Sanderlin, A.B.; Seymour, J.D.; Codd, S.L.

    In: Biotechnology and Bioengineering, Vol. 110, No. 5, 2013, p. 1366-1375.

    Research output: Contribution to journalArticle

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    AU - Vogt, Sarah

    AU - Sanderlin, A.B.

    AU - Seymour, J.D.

    AU - Codd, S.L.

    PY - 2013

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    AB - Biofilm growth in porous media is difficult to study non-invasively due to the opaqueness and heterogeneity of the systems. Magnetic resonance is utilized to non-invasively study water dynamics within porous media. Displacement-relaxation correlation experiments were performed on fluid flow during biofilm growth in a model porous media of mono-dispersed polystyrene beads. The spin-spin T2 magnetic relaxation distinguishes between the biofilm phase and bulk fluid phase due to water-biopolymer interactions present in the biofilm, and the flow dynamics are measured using PGSE NMR experiments. By correlating these two measurements, the effects of biofilm growth on the fluid dynamics can be separated into a detailed analysis of both the biofilm phase and the fluid phase simultaneously within the same experiment. Within the displacement resolution of these experiments, no convective flow was measured through the biomass. An increased amount of longitudinal hydrodynamic dispersion indicates increased hydrodynamic mixing due to fluid channeling caused by biofilm growth. The effect of different biofilm growth conditions was measured by varying the strength of the bacterial growth medium. Biotechnol. Bioeng. 2013; 110: 1366-1375. © 2012 Wiley Periodicals, Inc.

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