Peanut allergens are rapidly transferred in human breast milk and can prevent sensitization in mice

H. Bernard, S. Ah-Leung, M. F. Drumare, C. Feraudet-Tarisse, V. Verhasselt, J. M. Wal, C. Créminon, K. Adel-Patient

Research output: Contribution to journalArticle

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Abstract

Background Food allergens have been evidenced in breast milk under physiological conditions, but the kinetic and the role of this passage in food allergies are still unclear. We then aimed to analyze the passage of peanut allergens in human breast milk and their allergenicity/immunomodulatory properties. Methods Human breast milk was collected from two non-atopic peanut-tolerant mothers before and at different time points after ingestion of 30 g of commercial roasted peanut. Ara h 6, Ara h 6 immune complexes, and the IgE binding capacity of breast milk samples were measured using specific immunoassays. Their allergenic functionality was then assessed using cell-based assay. Finally, human breast milk obtained before or after peanut ingestion was administered intragastrically to BALB/c mice at different ages, and mice were further experimentally sensitized to peanut using cholera toxin. Results Ara h 6 is detected as soon as 10 min after peanut ingestion, with peak values observed within the first hour after ingestion. The transfer is long-lasting, small quantities of peanut allergens being detected over a 24-h period. IgG-Ara h 6 and IgA-Ara h 6 immune complexes are evidenced, following a different kinetic of excretion than free allergens. Peanut allergens transferred in milk are IgE reactive and can induce an allergic reaction in vitro. However, administration of human breast milk to young mice, notably before weaning, does not lead to sensitization, but instead to partial oral tolerance. Conclusion The low quantities of immunologically active allergens transferred through breast milk may prevent instead of priming allergic sensitization to peanut.

Original languageEnglish
Pages (from-to)888-897
Number of pages10
JournalAllergy: European Journal of Allergy and Clinical Immunology
Volume69
Issue number7
DOIs
Publication statusPublished - 2014
Externally publishedYes

Fingerprint

Human Milk
Allergens
Eating
Antigen-Antibody Complex
Immunoglobulin E
Arachis
Food Hypersensitivity
Cholera Toxin
Weaning
Immunoassay
Immunoglobulin A
Hypersensitivity
Milk
Immunoglobulin G
Food

Cite this

Bernard, H. ; Ah-Leung, S. ; Drumare, M. F. ; Feraudet-Tarisse, C. ; Verhasselt, V. ; Wal, J. M. ; Créminon, C. ; Adel-Patient, K. / Peanut allergens are rapidly transferred in human breast milk and can prevent sensitization in mice. In: Allergy: European Journal of Allergy and Clinical Immunology. 2014 ; Vol. 69, No. 7. pp. 888-897.
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abstract = "Background Food allergens have been evidenced in breast milk under physiological conditions, but the kinetic and the role of this passage in food allergies are still unclear. We then aimed to analyze the passage of peanut allergens in human breast milk and their allergenicity/immunomodulatory properties. Methods Human breast milk was collected from two non-atopic peanut-tolerant mothers before and at different time points after ingestion of 30 g of commercial roasted peanut. Ara h 6, Ara h 6 immune complexes, and the IgE binding capacity of breast milk samples were measured using specific immunoassays. Their allergenic functionality was then assessed using cell-based assay. Finally, human breast milk obtained before or after peanut ingestion was administered intragastrically to BALB/c mice at different ages, and mice were further experimentally sensitized to peanut using cholera toxin. Results Ara h 6 is detected as soon as 10 min after peanut ingestion, with peak values observed within the first hour after ingestion. The transfer is long-lasting, small quantities of peanut allergens being detected over a 24-h period. IgG-Ara h 6 and IgA-Ara h 6 immune complexes are evidenced, following a different kinetic of excretion than free allergens. Peanut allergens transferred in milk are IgE reactive and can induce an allergic reaction in vitro. However, administration of human breast milk to young mice, notably before weaning, does not lead to sensitization, but instead to partial oral tolerance. Conclusion The low quantities of immunologically active allergens transferred through breast milk may prevent instead of priming allergic sensitization to peanut.",
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Bernard, H, Ah-Leung, S, Drumare, MF, Feraudet-Tarisse, C, Verhasselt, V, Wal, JM, Créminon, C & Adel-Patient, K 2014, 'Peanut allergens are rapidly transferred in human breast milk and can prevent sensitization in mice' Allergy: European Journal of Allergy and Clinical Immunology, vol. 69, no. 7, pp. 888-897. https://doi.org/10.1111/all.12411

Peanut allergens are rapidly transferred in human breast milk and can prevent sensitization in mice. / Bernard, H.; Ah-Leung, S.; Drumare, M. F.; Feraudet-Tarisse, C.; Verhasselt, V.; Wal, J. M.; Créminon, C.; Adel-Patient, K.

In: Allergy: European Journal of Allergy and Clinical Immunology, Vol. 69, No. 7, 2014, p. 888-897.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Peanut allergens are rapidly transferred in human breast milk and can prevent sensitization in mice

AU - Bernard, H.

AU - Ah-Leung, S.

AU - Drumare, M. F.

AU - Feraudet-Tarisse, C.

AU - Verhasselt, V.

AU - Wal, J. M.

AU - Créminon, C.

AU - Adel-Patient, K.

PY - 2014

Y1 - 2014

N2 - Background Food allergens have been evidenced in breast milk under physiological conditions, but the kinetic and the role of this passage in food allergies are still unclear. We then aimed to analyze the passage of peanut allergens in human breast milk and their allergenicity/immunomodulatory properties. Methods Human breast milk was collected from two non-atopic peanut-tolerant mothers before and at different time points after ingestion of 30 g of commercial roasted peanut. Ara h 6, Ara h 6 immune complexes, and the IgE binding capacity of breast milk samples were measured using specific immunoassays. Their allergenic functionality was then assessed using cell-based assay. Finally, human breast milk obtained before or after peanut ingestion was administered intragastrically to BALB/c mice at different ages, and mice were further experimentally sensitized to peanut using cholera toxin. Results Ara h 6 is detected as soon as 10 min after peanut ingestion, with peak values observed within the first hour after ingestion. The transfer is long-lasting, small quantities of peanut allergens being detected over a 24-h period. IgG-Ara h 6 and IgA-Ara h 6 immune complexes are evidenced, following a different kinetic of excretion than free allergens. Peanut allergens transferred in milk are IgE reactive and can induce an allergic reaction in vitro. However, administration of human breast milk to young mice, notably before weaning, does not lead to sensitization, but instead to partial oral tolerance. Conclusion The low quantities of immunologically active allergens transferred through breast milk may prevent instead of priming allergic sensitization to peanut.

AB - Background Food allergens have been evidenced in breast milk under physiological conditions, but the kinetic and the role of this passage in food allergies are still unclear. We then aimed to analyze the passage of peanut allergens in human breast milk and their allergenicity/immunomodulatory properties. Methods Human breast milk was collected from two non-atopic peanut-tolerant mothers before and at different time points after ingestion of 30 g of commercial roasted peanut. Ara h 6, Ara h 6 immune complexes, and the IgE binding capacity of breast milk samples were measured using specific immunoassays. Their allergenic functionality was then assessed using cell-based assay. Finally, human breast milk obtained before or after peanut ingestion was administered intragastrically to BALB/c mice at different ages, and mice were further experimentally sensitized to peanut using cholera toxin. Results Ara h 6 is detected as soon as 10 min after peanut ingestion, with peak values observed within the first hour after ingestion. The transfer is long-lasting, small quantities of peanut allergens being detected over a 24-h period. IgG-Ara h 6 and IgA-Ara h 6 immune complexes are evidenced, following a different kinetic of excretion than free allergens. Peanut allergens transferred in milk are IgE reactive and can induce an allergic reaction in vitro. However, administration of human breast milk to young mice, notably before weaning, does not lead to sensitization, but instead to partial oral tolerance. Conclusion The low quantities of immunologically active allergens transferred through breast milk may prevent instead of priming allergic sensitization to peanut.

KW - breast-feeding

KW - food antigens

KW - peanut allergy

KW - primary prevention

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U2 - 10.1111/all.12411

DO - 10.1111/all.12411

M3 - Article

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