Paraspeckles: Where Long Noncoding RNA Meets Phase Separation

Archa H Fox, Shinichi Nakagawa, Tetsuro Hirose, Charles S Bond

Research output: Contribution to journalReview article

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Abstract

Long noncoding RNA (lncRNA) molecules are some of the newest and least understood players in gene regulation. Hence, we need good model systems with well-defined RNA and protein components. One such system is paraspeckles - protein-rich nuclear organelles built around a specific lncRNA scaffold. New discoveries show how paraspeckles are formed through multiple RNA-protein and protein-protein interactions, some of which involve extensive polymerization, and others with multivalent interactions driving phase separation. Once formed, paraspeckles influence gene regulation through sequestration of component proteins and RNAs, with subsequent depletion in other compartments. Here we focus on the dual aspects of paraspeckle structure and function, revealing an emerging role for these dynamic bodies in a multitude of cellular settings.

LanguageEnglish
Pages124-135
Number of pages12
JournalTrends in Biochemical Sciences
Volume43
Issue number2
DOIs
StatePublished - Feb 2018

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Long Noncoding RNA
Phase separation
RNA
Proteins
Gene expression
Nuclear Proteins
Polymerization
Organelles
Genes
Scaffolds
Molecules

Cite this

Fox, Archa H ; Nakagawa, Shinichi ; Hirose, Tetsuro ; Bond, Charles S. / Paraspeckles : Where Long Noncoding RNA Meets Phase Separation. In: Trends in Biochemical Sciences. 2018 ; Vol. 43, No. 2. pp. 124-135
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Paraspeckles : Where Long Noncoding RNA Meets Phase Separation. / Fox, Archa H; Nakagawa, Shinichi; Hirose, Tetsuro; Bond, Charles S.

In: Trends in Biochemical Sciences, Vol. 43, No. 2, 02.2018, p. 124-135.

Research output: Contribution to journalReview article

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T2 - Trends in Biochemical Sciences

AU - Fox,Archa H

AU - Nakagawa,Shinichi

AU - Hirose,Tetsuro

AU - Bond,Charles S

N1 - Copyright © 2017 Elsevier Ltd. All rights reserved.

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N2 - Long noncoding RNA (lncRNA) molecules are some of the newest and least understood players in gene regulation. Hence, we need good model systems with well-defined RNA and protein components. One such system is paraspeckles - protein-rich nuclear organelles built around a specific lncRNA scaffold. New discoveries show how paraspeckles are formed through multiple RNA-protein and protein-protein interactions, some of which involve extensive polymerization, and others with multivalent interactions driving phase separation. Once formed, paraspeckles influence gene regulation through sequestration of component proteins and RNAs, with subsequent depletion in other compartments. Here we focus on the dual aspects of paraspeckle structure and function, revealing an emerging role for these dynamic bodies in a multitude of cellular settings.

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