Pan-serotype diagnostic for foot-and-mouth disease using the consensus antigen of nonstructural protein 3B

Alyssa K. Van Dreumel, Wojtek P. Michalski, Leanne M. McNabb, Brian J. Shiell, Nagendrakumar B. Singanallur, Grantley R. Peck

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

An amino acid consensus sequence for the seven serotypes of foot-and-mouth disease virus (FMDV) nonstructural protein 3B, including all three contiguous repeats, and its use in the development of a pan-serotype diagnostic test for all seven FMDV serotypes are described. The amino acid consensus sequence of the 3B protein was determined from a multiple-sequence alignment of 125 sequences of 3B. The consensus 3B (c3B) protein was expressed as a soluble recombinant fusion protein with maltose-binding protein (MBP) using a bacterial expression system and was affinity purified using amylose resin. The MBP-c3B protein was used as the antigen in the development of a competition enzyme-linked immunosorbent assay (cELISA) for detection of anti-3B antibodies in bovine sera. The comparative diagnostic sensitivity and specificity at 47% inhibition were estimated to be 87.22% and 93.15%, respectively. Reactivity of c3B with bovine sera representing the seven FMDV serotypes demonstrated the pan-serotype diagnostic capability of this bioreagent. The consensus antigen and competition ELISA are described here as candidates for a pan-serotype diagnostic test for FMDV infection.
Original languageEnglish
Pages (from-to)1797-1805
Number of pages9
JournalJournal of Clinical Microbiology
Volume53
Issue number6
DOIs
Publication statusPublished - Jun 2015
Externally publishedYes

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