TY - JOUR
T1 - Overexpression of MsSAG113 gene promotes leaf senescence in alfalfa via participating in the hormone regulatory network
AU - Li, Shuwen
AU - Xie, Hong
AU - Zhou, Lingfang
AU - Dong, Di
AU - Liu, Yaling
AU - Jia, Chenyan
AU - Han, Liebao
AU - Chao, Yuehui
AU - Chen, Yinglong
N1 - Funding Information:
This work was supported by the Inner Mongolia Science and Technology Plan “Jiebangguashuai” Project (Breeding and Industrialization Demonstration of New Alfalfa Varieties with High Quality) and the Fundamental Research Funds for the Central Universities (Grant No. 2021ZY84).
Publisher Copyright:
Copyright © 2022 Li, Xie, Zhou, Dong, Liu, Jia, Han, Chao and Chen.
PY - 2022/12/8
Y1 - 2022/12/8
N2 - INTRODUCTION: Alfalfa (Medicago sativa) is a kind of high quality leguminous forage species, which was widely cultivated in the world. Leaf senescence is an essential process in plant development and life cycle. Here, we reported the isolation and functional analysis of an alfalfa SENESCENCE-ASSOCIATED GENE113 (MsSAG113), which belongs to the PP2C family and mainly plays a role in promoting plant senescence.METHODS: In the study, Agrobacterium-mediated, gene expression analysis, next generation sequencing, DNA pull-down, yeast single hybridization and transient expression were used to identify the function of MsSAG113 gene.RESULTS: The MsSAG113 gene was isolated from alfalfa, and the transgenic plants were obtained by Agrobacterium-mediated method. Compared with the wildtype, transgenic plants showed premature senescence in leaves, especially when cultivated under dark conditions. Meanwhile, application of exogenous hormones ABA, SA, MeJA, obviously acclerated leaf senescence of transgenic plants. Furthermore, the detached leaves from transgenic plants turned yellow earlier with lower chlorophyll content. Transcriptome analysis identified a total of 1,392 differentially expressed genes (DEGs), involving 13 transcription factor families. Of which, 234 genes were related to phytohormone synthesis, metabolism and transduction. Pull-down assay and yeast one-hybrid assay confirmed that alfalfa zinc finger CCCH domain-containing protein 39 (MsC3H-39) could directly bind the upstream of MsSAG113 gene. In conclusion, the MsSAG113 gene plays a crucial role in promoting leaf senescence in alfalfa via participating in the hormone regulatory network.DISCUSSION: This provides an essential basis for further analysis on the regulatory network involving senescence-associated genes in alfalfa.
AB - INTRODUCTION: Alfalfa (Medicago sativa) is a kind of high quality leguminous forage species, which was widely cultivated in the world. Leaf senescence is an essential process in plant development and life cycle. Here, we reported the isolation and functional analysis of an alfalfa SENESCENCE-ASSOCIATED GENE113 (MsSAG113), which belongs to the PP2C family and mainly plays a role in promoting plant senescence.METHODS: In the study, Agrobacterium-mediated, gene expression analysis, next generation sequencing, DNA pull-down, yeast single hybridization and transient expression were used to identify the function of MsSAG113 gene.RESULTS: The MsSAG113 gene was isolated from alfalfa, and the transgenic plants were obtained by Agrobacterium-mediated method. Compared with the wildtype, transgenic plants showed premature senescence in leaves, especially when cultivated under dark conditions. Meanwhile, application of exogenous hormones ABA, SA, MeJA, obviously acclerated leaf senescence of transgenic plants. Furthermore, the detached leaves from transgenic plants turned yellow earlier with lower chlorophyll content. Transcriptome analysis identified a total of 1,392 differentially expressed genes (DEGs), involving 13 transcription factor families. Of which, 234 genes were related to phytohormone synthesis, metabolism and transduction. Pull-down assay and yeast one-hybrid assay confirmed that alfalfa zinc finger CCCH domain-containing protein 39 (MsC3H-39) could directly bind the upstream of MsSAG113 gene. In conclusion, the MsSAG113 gene plays a crucial role in promoting leaf senescence in alfalfa via participating in the hormone regulatory network.DISCUSSION: This provides an essential basis for further analysis on the regulatory network involving senescence-associated genes in alfalfa.
KW - alfalfa
KW - MsC3H-39
KW - MsSAG113
KW - RNA-seq
KW - senescence
UR - http://www.scopus.com/inward/record.url?scp=85144926310&partnerID=8YFLogxK
U2 - 10.3389/fpls.2022.1085497
DO - 10.3389/fpls.2022.1085497
M3 - Article
C2 - 36570962
SN - 1664-462X
VL - 13
JO - Frontiers in Plant Science
JF - Frontiers in Plant Science
M1 - 1085497
ER -