Overcoming physiological dormancy in seeds of Triodia (Poaceae) to improve restoration in the arid zone

Todd Erickson, Nancy A. Shackelford, Kingsley W. Dixon, Shane R. Turner, David J. Merritt

    Research output: Contribution to journalArticle

    23 Citations (Scopus)

    Abstract

    Reinstating dominant Triodia grassland communities following disturbance has been a focus of arid land restoration practitioners for decades in Australia. Yet, seed quality and variable seed germination have seriously hindered the reestablishment potential of Triodia species to date. This study set out to examine diaspore quality, germination requirements, and seed dormancy in seven Triodia species to identify first, then resolve, germination impediments. Freshly collected florets from all species were cleaned to ensure that each floret contained a viable seed and then evaluated for their initial germination capacity. Very low germination from florets (<10%) indicated the presence of physiological seed dormancy (PD) in all species. However, germination was significantly improved (up to 57%) with the use of 0.67 µm karrikinolide (KAR1), and to a lesser degree with 289 µm gibberellic acid (GA3). When the covering floret structures (i.e. lemma and palea) were removed, germination increased up to 59%, which was further enhanced after exposure to GA3 (up to 66%) and KAR1 (up to 92%). Optimal germination temperatures varied from 20 to 35°C and were species specific. Dry after-ripening (DAR −30°C and 50% relative humidity [RH]) of florets and seeds promoted the progressive loss of PD over 12–24 months storage for most species. Germination, dormancy level, and response to incubation temperature differed amongst species, experimental units (florets and seeds), DAR treatments, and after exposure to germination stimulants (GA3 and KAR1). For use in restoration seeding programs, careful consideration of seed pre-treatments is necessary to improve germination in Triodia species. © 2016 Society for Ecological Restoration
    Original languageEnglish
    Pages (from-to)64 - 76
    Number of pages13
    JournalRestoration Ecology
    Volume24
    Issue number2
    DOIs
    Publication statusPublished - 2016

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    Triodia
    seed dormancy
    dormancy
    Poaceae
    arid zones
    germination
    seed
    florets
    seeds
    restoration
    after-ripening
    seed treatment
    ecological restoration
    diaspore
    land restoration
    seed quality
    ripening
    gibberellic acid
    arid lands
    seeding

    Cite this

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    title = "Overcoming physiological dormancy in seeds of Triodia (Poaceae) to improve restoration in the arid zone",
    abstract = "Reinstating dominant Triodia grassland communities following disturbance has been a focus of arid land restoration practitioners for decades in Australia. Yet, seed quality and variable seed germination have seriously hindered the reestablishment potential of Triodia species to date. This study set out to examine diaspore quality, germination requirements, and seed dormancy in seven Triodia species to identify first, then resolve, germination impediments. Freshly collected florets from all species were cleaned to ensure that each floret contained a viable seed and then evaluated for their initial germination capacity. Very low germination from florets (<10{\%}) indicated the presence of physiological seed dormancy (PD) in all species. However, germination was significantly improved (up to 57{\%}) with the use of 0.67 µm karrikinolide (KAR1), and to a lesser degree with 289 µm gibberellic acid (GA3). When the covering floret structures (i.e. lemma and palea) were removed, germination increased up to 59{\%}, which was further enhanced after exposure to GA3 (up to 66{\%}) and KAR1 (up to 92{\%}). Optimal germination temperatures varied from 20 to 35°C and were species specific. Dry after-ripening (DAR −30°C and 50{\%} relative humidity [RH]) of florets and seeds promoted the progressive loss of PD over 12–24 months storage for most species. Germination, dormancy level, and response to incubation temperature differed amongst species, experimental units (florets and seeds), DAR treatments, and after exposure to germination stimulants (GA3 and KAR1). For use in restoration seeding programs, careful consideration of seed pre-treatments is necessary to improve germination in Triodia species. {\circledC} 2016 Society for Ecological Restoration",
    author = "Todd Erickson and Shackelford, {Nancy A.} and Dixon, {Kingsley W.} and Turner, {Shane R.} and Merritt, {David J.}",
    year = "2016",
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    }

    Overcoming physiological dormancy in seeds of Triodia (Poaceae) to improve restoration in the arid zone. / Erickson, Todd; Shackelford, Nancy A.; Dixon, Kingsley W.; Turner, Shane R.; Merritt, David J.

    In: Restoration Ecology, Vol. 24, No. 2, 2016, p. 64 - 76.

    Research output: Contribution to journalArticle

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    AU - Erickson, Todd

    AU - Shackelford, Nancy A.

    AU - Dixon, Kingsley W.

    AU - Turner, Shane R.

    AU - Merritt, David J.

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    AB - Reinstating dominant Triodia grassland communities following disturbance has been a focus of arid land restoration practitioners for decades in Australia. Yet, seed quality and variable seed germination have seriously hindered the reestablishment potential of Triodia species to date. This study set out to examine diaspore quality, germination requirements, and seed dormancy in seven Triodia species to identify first, then resolve, germination impediments. Freshly collected florets from all species were cleaned to ensure that each floret contained a viable seed and then evaluated for their initial germination capacity. Very low germination from florets (<10%) indicated the presence of physiological seed dormancy (PD) in all species. However, germination was significantly improved (up to 57%) with the use of 0.67 µm karrikinolide (KAR1), and to a lesser degree with 289 µm gibberellic acid (GA3). When the covering floret structures (i.e. lemma and palea) were removed, germination increased up to 59%, which was further enhanced after exposure to GA3 (up to 66%) and KAR1 (up to 92%). Optimal germination temperatures varied from 20 to 35°C and were species specific. Dry after-ripening (DAR −30°C and 50% relative humidity [RH]) of florets and seeds promoted the progressive loss of PD over 12–24 months storage for most species. Germination, dormancy level, and response to incubation temperature differed amongst species, experimental units (florets and seeds), DAR treatments, and after exposure to germination stimulants (GA3 and KAR1). For use in restoration seeding programs, careful consideration of seed pre-treatments is necessary to improve germination in Triodia species. © 2016 Society for Ecological Restoration

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    SN - 1061-2971

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