Ostrich specific semen diluent and sperm motility characteristics during in vitro storage

A. M.J. Smith, M. Bonato, K. Dzama, I. A. Malecki, S. W.P. Cloete

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The dilution of semen is a very important initial process for semen processing and evaluation, storage and preservation in vitro and efficient artificial insemination. The aim of the study was to evaluate the effect of two synthetic diluents (OS1 and OS2) on ostrich sperm motility parameters during in vitro storage. Formulation of OS1 was based on macro minerals (Na, K, P, Ca, Mg) and OS2 on the further addition of micro minerals (Se and Zn), based on mineral concentration determined in the ostrich seminal plasma (SP). Sperm motility was evaluated at different processing stages (neat, after dilution, during storage and after storage) by measuring several sperm motility variables using the Sperm Class Analyzer® (SCA). Processing (dilution, cooling and storage) of semen for in vitro storage purposes decreased the values for all sperm motility variables measured. The percentage motile (MOT) and progressive motile (PMOT) sperm decreased 20% to 30% during 24 h of storage, independent of diluent type. Quality of sperm swim (LIN, STR and WOB), however, was sustained during the longer storage periods (48 h) with the OS2 diluent modified with Se and Zn additions. Quality of sperm swim with use of OS1 was 6% to 8% less for the LIN, STR, and WOB variables. Male fitted as a fixed effect accounted for >60% of the variation for certain sperm motility variables (PMOT, MOT, VCL, VSL, VAP and ALH) evaluated at different processing stages. Semen from specific males had sustained sperm motility characteristics to a greater extent than that of other males during the 24-h storage period.

Original languageEnglish
Pages (from-to)107-116
Number of pages10
JournalAnimal Reproduction Science
Volume193
DOIs
Publication statusPublished - 1 Jun 2018

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Struthioniformes
semen extenders
ostriches
Sperm Motility
Semen
sperm motility
Spermatozoa
semen
Minerals
spermatozoa
processing stages
storage time
Artificial Insemination
minerals
seminal plasma
In Vitro Techniques
artificial insemination
mineral content
cooling

Cite this

Smith, A. M.J. ; Bonato, M. ; Dzama, K. ; Malecki, I. A. ; Cloete, S. W.P. / Ostrich specific semen diluent and sperm motility characteristics during in vitro storage. In: Animal Reproduction Science. 2018 ; Vol. 193. pp. 107-116.
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Ostrich specific semen diluent and sperm motility characteristics during in vitro storage. / Smith, A. M.J.; Bonato, M.; Dzama, K.; Malecki, I. A.; Cloete, S. W.P.

In: Animal Reproduction Science, Vol. 193, 01.06.2018, p. 107-116.

Research output: Contribution to journalArticle

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T1 - Ostrich specific semen diluent and sperm motility characteristics during in vitro storage

AU - Smith, A. M.J.

AU - Bonato, M.

AU - Dzama, K.

AU - Malecki, I. A.

AU - Cloete, S. W.P.

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AB - The dilution of semen is a very important initial process for semen processing and evaluation, storage and preservation in vitro and efficient artificial insemination. The aim of the study was to evaluate the effect of two synthetic diluents (OS1 and OS2) on ostrich sperm motility parameters during in vitro storage. Formulation of OS1 was based on macro minerals (Na, K, P, Ca, Mg) and OS2 on the further addition of micro minerals (Se and Zn), based on mineral concentration determined in the ostrich seminal plasma (SP). Sperm motility was evaluated at different processing stages (neat, after dilution, during storage and after storage) by measuring several sperm motility variables using the Sperm Class Analyzer® (SCA). Processing (dilution, cooling and storage) of semen for in vitro storage purposes decreased the values for all sperm motility variables measured. The percentage motile (MOT) and progressive motile (PMOT) sperm decreased 20% to 30% during 24 h of storage, independent of diluent type. Quality of sperm swim (LIN, STR and WOB), however, was sustained during the longer storage periods (48 h) with the OS2 diluent modified with Se and Zn additions. Quality of sperm swim with use of OS1 was 6% to 8% less for the LIN, STR, and WOB variables. Male fitted as a fixed effect accounted for >60% of the variation for certain sperm motility variables (PMOT, MOT, VCL, VSL, VAP and ALH) evaluated at different processing stages. Semen from specific males had sustained sperm motility characteristics to a greater extent than that of other males during the 24-h storage period.

KW - Artificial insemination

KW - In vitro

KW - Ostrich semen extender

KW - Semen dilution

KW - Semen storage

KW - Sperm function

KW - Sperm motility

KW - Struthio camelus

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