Osmotic adjustment segregates with and is positively related to seed yield in F3 lines of crosses between Brassica napus and B.juncea subjected to water deficit

Qifu Ma, David Turner

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    19 Citations (Scopus)

    Abstract

    For osmotic adjustment to be used as a selection criterion for adaptation to drought there is a need to demonstrate its segregation and association with seed yield in a population. This study examined osmotic adjustment and seed yield in F3 lines derived from crosses between Brassica napus (cvv. Karoo, Monty) and B. juncea (line JN25). A clear separation of the level of osmotic adjustment was observed among 20 F3 lines of Karoo x JN25 (7 high and 13 low) and 20 F3 lines of Monty x JN25 (8 high and 12 low) under glasshouse conditions. When the 3 parents and 8 selected F3 lines were grown in a low-rainfall environment in the field, the segregation of osmotic adjustment was largely similar to that observed in the glasshouse. Yield reduction, with irrigated plots as controls, was up to 30% for genotypes with low osmotic adjustment but only 10% for those with high osmotic adjustment. Osmotic adjustment was closely correlated with the accumulation of K+ (r = 0.91), soluble sugars (r = 0.90) and proline (r = 0.96), whereas other solutes (Na+, NH4+, Cl-, NO3-, Mg-2(+) and Ca-2(+)) made little or no contribution to osmotic adjustment. Proline concentrations were very low in well-watered plants but sharply increased by 5- to 15-fold in plants subjected to water deficit, and net proline accumulation showed a higher association with total seed number (r = 0.86) and yield (r = 0.89) than did the concentrations of K+ (r = 0.75 to 0.82) or soluble sugars (r = 0.68 to 0.72). The study suggests that leaf proline concentration could be a good indicator of osmotic adjustment in Brassica oilseeds.
    Original languageEnglish
    Pages (from-to)1621-1627
    JournalAustralian Journal of Experimental Agriculture
    Volume46
    Issue number12
    DOIs
    Publication statusPublished - 2006

    Fingerprint

    Brassica napus
    Proline
    seed yield
    proline
    Seeds
    Water
    water
    sugars
    greenhouses
    Brassica
    Droughts
    oilseeds
    Brassica juncea
    selection criteria
    Patient Selection
    solutes
    Genotype
    drought
    rain
    genotype

    Cite this

    @article{44c61151d6e04d00933aef04ad5a6cfa,
    title = "Osmotic adjustment segregates with and is positively related to seed yield in F3 lines of crosses between Brassica napus and B.juncea subjected to water deficit",
    abstract = "For osmotic adjustment to be used as a selection criterion for adaptation to drought there is a need to demonstrate its segregation and association with seed yield in a population. This study examined osmotic adjustment and seed yield in F3 lines derived from crosses between Brassica napus (cvv. Karoo, Monty) and B. juncea (line JN25). A clear separation of the level of osmotic adjustment was observed among 20 F3 lines of Karoo x JN25 (7 high and 13 low) and 20 F3 lines of Monty x JN25 (8 high and 12 low) under glasshouse conditions. When the 3 parents and 8 selected F3 lines were grown in a low-rainfall environment in the field, the segregation of osmotic adjustment was largely similar to that observed in the glasshouse. Yield reduction, with irrigated plots as controls, was up to 30{\%} for genotypes with low osmotic adjustment but only 10{\%} for those with high osmotic adjustment. Osmotic adjustment was closely correlated with the accumulation of K+ (r = 0.91), soluble sugars (r = 0.90) and proline (r = 0.96), whereas other solutes (Na+, NH4+, Cl-, NO3-, Mg-2(+) and Ca-2(+)) made little or no contribution to osmotic adjustment. Proline concentrations were very low in well-watered plants but sharply increased by 5- to 15-fold in plants subjected to water deficit, and net proline accumulation showed a higher association with total seed number (r = 0.86) and yield (r = 0.89) than did the concentrations of K+ (r = 0.75 to 0.82) or soluble sugars (r = 0.68 to 0.72). The study suggests that leaf proline concentration could be a good indicator of osmotic adjustment in Brassica oilseeds.",
    author = "Qifu Ma and David Turner",
    year = "2006",
    doi = "10.1071/EA05247",
    language = "English",
    volume = "46",
    pages = "1621--1627",
    journal = "Australian Journal of Experimental Agriculture",
    issn = "0816-1089",
    publisher = "CSIRO Publishing",
    number = "12",

    }

    TY - JOUR

    T1 - Osmotic adjustment segregates with and is positively related to seed yield in F3 lines of crosses between Brassica napus and B.juncea subjected to water deficit

    AU - Ma, Qifu

    AU - Turner, David

    PY - 2006

    Y1 - 2006

    N2 - For osmotic adjustment to be used as a selection criterion for adaptation to drought there is a need to demonstrate its segregation and association with seed yield in a population. This study examined osmotic adjustment and seed yield in F3 lines derived from crosses between Brassica napus (cvv. Karoo, Monty) and B. juncea (line JN25). A clear separation of the level of osmotic adjustment was observed among 20 F3 lines of Karoo x JN25 (7 high and 13 low) and 20 F3 lines of Monty x JN25 (8 high and 12 low) under glasshouse conditions. When the 3 parents and 8 selected F3 lines were grown in a low-rainfall environment in the field, the segregation of osmotic adjustment was largely similar to that observed in the glasshouse. Yield reduction, with irrigated plots as controls, was up to 30% for genotypes with low osmotic adjustment but only 10% for those with high osmotic adjustment. Osmotic adjustment was closely correlated with the accumulation of K+ (r = 0.91), soluble sugars (r = 0.90) and proline (r = 0.96), whereas other solutes (Na+, NH4+, Cl-, NO3-, Mg-2(+) and Ca-2(+)) made little or no contribution to osmotic adjustment. Proline concentrations were very low in well-watered plants but sharply increased by 5- to 15-fold in plants subjected to water deficit, and net proline accumulation showed a higher association with total seed number (r = 0.86) and yield (r = 0.89) than did the concentrations of K+ (r = 0.75 to 0.82) or soluble sugars (r = 0.68 to 0.72). The study suggests that leaf proline concentration could be a good indicator of osmotic adjustment in Brassica oilseeds.

    AB - For osmotic adjustment to be used as a selection criterion for adaptation to drought there is a need to demonstrate its segregation and association with seed yield in a population. This study examined osmotic adjustment and seed yield in F3 lines derived from crosses between Brassica napus (cvv. Karoo, Monty) and B. juncea (line JN25). A clear separation of the level of osmotic adjustment was observed among 20 F3 lines of Karoo x JN25 (7 high and 13 low) and 20 F3 lines of Monty x JN25 (8 high and 12 low) under glasshouse conditions. When the 3 parents and 8 selected F3 lines were grown in a low-rainfall environment in the field, the segregation of osmotic adjustment was largely similar to that observed in the glasshouse. Yield reduction, with irrigated plots as controls, was up to 30% for genotypes with low osmotic adjustment but only 10% for those with high osmotic adjustment. Osmotic adjustment was closely correlated with the accumulation of K+ (r = 0.91), soluble sugars (r = 0.90) and proline (r = 0.96), whereas other solutes (Na+, NH4+, Cl-, NO3-, Mg-2(+) and Ca-2(+)) made little or no contribution to osmotic adjustment. Proline concentrations were very low in well-watered plants but sharply increased by 5- to 15-fold in plants subjected to water deficit, and net proline accumulation showed a higher association with total seed number (r = 0.86) and yield (r = 0.89) than did the concentrations of K+ (r = 0.75 to 0.82) or soluble sugars (r = 0.68 to 0.72). The study suggests that leaf proline concentration could be a good indicator of osmotic adjustment in Brassica oilseeds.

    U2 - 10.1071/EA05247

    DO - 10.1071/EA05247

    M3 - Article

    VL - 46

    SP - 1621

    EP - 1627

    JO - Australian Journal of Experimental Agriculture

    JF - Australian Journal of Experimental Agriculture

    SN - 0816-1089

    IS - 12

    ER -