Oral tolerance is inefficient in neonatal mice due to a physiological Vitamin A deficiency

M. Turfkruyer, A. Rekima, P. Macchiaverni, L. Le Bourhis, V. Muncan, G. R. Van Den Brink, M. K. Tulic, V. Verhasselt

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Increased risk of allergy during early life indicates deficient immune regulation in this period of life. To date, the cause for inefficient neonatal immune regulation has never been elucidated. We aimed to define the ontogeny of oral tolerance and to identify necessary conditions specific for this stage of life. Ovalbumin (OVA) was administered orally to mice through breast milk and efficiency of systemic tolerance to OVA was assessed in adulthood using a model of allergic airway inflammation. Oral tolerance induction was fully efficient starting third week of life. Inefficiency in neonates was a consequence of abnormal antigen transfer across the gut barrier and retinaldehyde dehydrogenase expression by mesenteric lymph node CD103 + neonatal dendritic cells, resulting in inefficient T-cell activation. Neonates' serum retinol levels were three times lower than in adult mice, and vitamin A supplementation was sufficient to rescue neonatal defects and allow tolerance induction from birth. The establishment of oral tolerance required the differentiation of Th1 lymphocytes in both vitamin A-supplemented neonates and 3-week-old unsupplemented mice. This knowledge should guide the design of interventions for allergy prevention that are adapted to the neonatal stage of life such as vitamin A supplementation.

Original languageEnglish
Pages (from-to)479-491
Number of pages13
JournalMucosal Immunology
Volume9
Issue number2
DOIs
Publication statusPublished - 1 Mar 2016
Externally publishedYes

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Vitamin A Deficiency
Vitamin A
Ovalbumin
Hypersensitivity
Retinaldehyde
Human Milk
Dendritic Cells
Oxidoreductases
Lymph Nodes
Parturition
Lymphocytes
Inflammation
T-Lymphocytes
Antigens
Serum

Cite this

Turfkruyer, M. ; Rekima, A. ; Macchiaverni, P. ; Le Bourhis, L. ; Muncan, V. ; Van Den Brink, G. R. ; Tulic, M. K. ; Verhasselt, V. / Oral tolerance is inefficient in neonatal mice due to a physiological Vitamin A deficiency. In: Mucosal Immunology. 2016 ; Vol. 9, No. 2. pp. 479-491.
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Turfkruyer, M, Rekima, A, Macchiaverni, P, Le Bourhis, L, Muncan, V, Van Den Brink, GR, Tulic, MK & Verhasselt, V 2016, 'Oral tolerance is inefficient in neonatal mice due to a physiological Vitamin A deficiency' Mucosal Immunology, vol. 9, no. 2, pp. 479-491. https://doi.org/10.1038/mi.2015.114

Oral tolerance is inefficient in neonatal mice due to a physiological Vitamin A deficiency. / Turfkruyer, M.; Rekima, A.; Macchiaverni, P.; Le Bourhis, L.; Muncan, V.; Van Den Brink, G. R.; Tulic, M. K.; Verhasselt, V.

In: Mucosal Immunology, Vol. 9, No. 2, 01.03.2016, p. 479-491.

Research output: Contribution to journalArticle

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Turfkruyer M, Rekima A, Macchiaverni P, Le Bourhis L, Muncan V, Van Den Brink GR et al. Oral tolerance is inefficient in neonatal mice due to a physiological Vitamin A deficiency. Mucosal Immunology. 2016 Mar 1;9(2):479-491. https://doi.org/10.1038/mi.2015.114