Oral Nucleotides only Minimally Improve 5-Fluorouracil-Induced Mucositis in Rats

Suzanne Mashtoub, B. Feo, A.L. Whittaker, K.A. Lymn, D. Martinez-Puig, G.S. Howarth

    Research output: Contribution to journalArticlepeer-review

    4 Citations (Scopus)
    249 Downloads (Pure)


    © 2015, Taylor & Francis Group, LLC. Chemotherapy-induced mucositis is characterized by inflammation and ulceration of the intestinal mucosa, compromising intestinal function. Exogenous nucleotides have been reported to repair the mucosa. The nucleotide preparation, Nucleoforce F0328 (Nucleoforce), was investigated for its potential to ameliorate intestinal mucositis in rats. Female Dark Agouti rats (n = 8/group) were gavaged once daily with Nucleoforce (175 mg/kg) or water from Days 0 to 8 and injected (i.p.) with 5-fluorouracil (5-FU; 150 mg/kg) or saline on Day 5. Histological parameters (disease severity, crypt depth, and villus height measurements) and myeloperoxidase activity were quantified. P <0.05 was considered significant. Jejunal and ileal histological disease severity scores were significantly increased by 5-FU, compared to normal controls (P <0.05). Nucleoforce treatment in 5-FU-injected rats significantly reduced jejunal and ileal disease severity compared to 5-FU controls (P <0.05). In 5-FU-injected rats, jejunal and ileal villus heights and crypt depths were significantly decreased compared to 5-FU controls, with no additional Nucleoforce effect (P > 0.05). Intestinal myeloperoxidase activity was significantly elevated by 5-FU (8.8-fold), compared to normal controls (P <0.05), which was not normalized by Nucleoforce treatment (P > 0.05). Nucleoforce only partially improved parameters associated with experimentally-induced mucositis. Future studies could investigate increased concentrations, more frequent administration, or protective microencapsulation delivery methods, to increase bioavailability.
    Original languageEnglish
    Pages (from-to)994-1000
    JournalNutrition and Cancer
    Issue number6
    Publication statusPublished - 18 Aug 2015


    Dive into the research topics of 'Oral Nucleotides only Minimally Improve 5-Fluorouracil-Induced Mucositis in Rats'. Together they form a unique fingerprint.

    Cite this