Optimising tympanic membrane wound healing on silk fibroin bioscaffold using bioactive molecules

Bing Teh

    Research output: ThesisDoctoral Thesis

    573 Downloads (Pure)

    Abstract

    [Truncated abstract] Tympanic membrane (TM) perforations are common, with current treatments for chronic perforations involving surgery using various graft materials. Recent research developments in this field have begun applying the principles of tissue engineering, with appropriate scaffolds, cells and bioactive molecules (BMs). This has revolutionised the therapeutic approach – due to the availability of a wide range of materials with appropriate compatibility and mechanical properties to regenerate the membrane acoustics – and may also represent a paradigm shift in the management of TM perforations in an outpatient setting without surgery.

    The aim of this thesis was to identify the types of silk fibroin (SF) scaffolds and BMs best suited for promoting wound healing. This was achieved by studying the interaction between human-derived TM (hTM) keratinocytes, various BMs and SF scaffolds.

    hTM keratinocytes were obtained, cultured and characterised. The effects of selected BMs and their combinations on hTM were examined for cell proliferation and migration. Three different SF scaffolds obtained from Bombyx mori (Mulberry), Philosamia cynthia ricini (Eri) and Antheraea assamensis (Muga) were used. hTM cultured on Mulberry, Eri and Muga SF was examined for cell morphology, adhesion, migration, proliferation, growth rate, cytotoxicity and viability. The effects of casting solvent of Mulberry SF were also evaluated. Finally, the effects of Mulberry SF and transforming growth factor-alpha (TGF-α) combination were studied in terms of TGF-α delivery.
    Original languageEnglish
    QualificationDoctor of Philosophy
    Publication statusUnpublished - 2013

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