Optical coherence micro-elastography: Mechanical-contrast imaging of tissue microstructure

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    Abstract

    We present optical coherence micro-elastography, an improved form of compression optical coherence elastography. We demonstrate the capacity of this technique to produce en face images, closely corresponding with histology, that reveal micro-scale mechanical contrast in human breast and lymph node tissues. We use phase-sensitive, three-dimensional optical coherence tomography (OCT) to probe the nanometer-to-micrometer-scale axial displacements in tissues induced by compressive loading. Optical coherence micro- elastography incorporates common-path interferometry, weighted averaging of the complex OCT signal and weighted least-squares regression. Using three-dimensional phase unwrapping, we have increased the maximum detectable strain eleven-fold over no unwrapping and the minimum detectable strain is 2.6 με. We demonstrate the potential of mechanical over optical contrast for visualizing micro-scale tissue structures in human breast cancer pathology and lymph node morphology. © 2014 Optical Society of America.
    Original languageEnglish
    Pages (from-to)2113-2124
    JournalBiomedical Optics Express
    Volume5
    Issue number7
    Early online date9 Jun 2014
    DOIs
    Publication statusPublished - Jul 2014

    Fingerprint

    Elasticity Imaging Techniques
    Optical Coherence Tomography
    microstructure
    Lymph Nodes
    Interferometry
    lymphatic system
    breast
    Least-Squares Analysis
    tomography
    Histology
    Breast
    histology
    Pathology
    Breast Neoplasms
    pathology
    micrometers
    regression analysis
    interferometry
    cancer
    probes

    Cite this

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    title = "Optical coherence micro-elastography: Mechanical-contrast imaging of tissue microstructure",
    abstract = "We present optical coherence micro-elastography, an improved form of compression optical coherence elastography. We demonstrate the capacity of this technique to produce en face images, closely corresponding with histology, that reveal micro-scale mechanical contrast in human breast and lymph node tissues. We use phase-sensitive, three-dimensional optical coherence tomography (OCT) to probe the nanometer-to-micrometer-scale axial displacements in tissues induced by compressive loading. Optical coherence micro- elastography incorporates common-path interferometry, weighted averaging of the complex OCT signal and weighted least-squares regression. Using three-dimensional phase unwrapping, we have increased the maximum detectable strain eleven-fold over no unwrapping and the minimum detectable strain is 2.6 με. We demonstrate the potential of mechanical over optical contrast for visualizing micro-scale tissue structures in human breast cancer pathology and lymph node morphology. {\circledC} 2014 Optical Society of America.",
    author = "Brendan Kennedy and Robert Mclaughlin and K.M. Kennedy and Lixin Chin and Andrea Curatolo and Alan Tien and B.B. Latham and Christobel Saunders and David Sampson",
    year = "2014",
    month = "7",
    doi = "10.1364/BOE.5.002113",
    language = "English",
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    pages = "2113--2124",
    journal = "Biomedical Optics Express",
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    TY - JOUR

    T1 - Optical coherence micro-elastography: Mechanical-contrast imaging of tissue microstructure

    AU - Kennedy, Brendan

    AU - Mclaughlin, Robert

    AU - Kennedy, K.M.

    AU - Chin, Lixin

    AU - Curatolo, Andrea

    AU - Tien, Alan

    AU - Latham, B.B.

    AU - Saunders, Christobel

    AU - Sampson, David

    PY - 2014/7

    Y1 - 2014/7

    N2 - We present optical coherence micro-elastography, an improved form of compression optical coherence elastography. We demonstrate the capacity of this technique to produce en face images, closely corresponding with histology, that reveal micro-scale mechanical contrast in human breast and lymph node tissues. We use phase-sensitive, three-dimensional optical coherence tomography (OCT) to probe the nanometer-to-micrometer-scale axial displacements in tissues induced by compressive loading. Optical coherence micro- elastography incorporates common-path interferometry, weighted averaging of the complex OCT signal and weighted least-squares regression. Using three-dimensional phase unwrapping, we have increased the maximum detectable strain eleven-fold over no unwrapping and the minimum detectable strain is 2.6 με. We demonstrate the potential of mechanical over optical contrast for visualizing micro-scale tissue structures in human breast cancer pathology and lymph node morphology. © 2014 Optical Society of America.

    AB - We present optical coherence micro-elastography, an improved form of compression optical coherence elastography. We demonstrate the capacity of this technique to produce en face images, closely corresponding with histology, that reveal micro-scale mechanical contrast in human breast and lymph node tissues. We use phase-sensitive, three-dimensional optical coherence tomography (OCT) to probe the nanometer-to-micrometer-scale axial displacements in tissues induced by compressive loading. Optical coherence micro- elastography incorporates common-path interferometry, weighted averaging of the complex OCT signal and weighted least-squares regression. Using three-dimensional phase unwrapping, we have increased the maximum detectable strain eleven-fold over no unwrapping and the minimum detectable strain is 2.6 με. We demonstrate the potential of mechanical over optical contrast for visualizing micro-scale tissue structures in human breast cancer pathology and lymph node morphology. © 2014 Optical Society of America.

    U2 - 10.1364/BOE.5.002113

    DO - 10.1364/BOE.5.002113

    M3 - Article

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    SP - 2113

    EP - 2124

    JO - Biomedical Optics Express

    JF - Biomedical Optics Express

    SN - 2156-7085

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    ER -