Optical coherence micro-elastography: Mechanical-contrast imaging of tissue microstructure

Brendan Kennedy, Robert Mclaughlin, K.M. Kennedy, Lixin Chin, Andrea Curatolo, Alan Tien, B.B. Latham, Christobel Saunders, David Sampson

    Research output: Contribution to journalArticle

    103 Citations (Scopus)

    Abstract

    We present optical coherence micro-elastography, an improved form of compression optical coherence elastography. We demonstrate the capacity of this technique to produce en face images, closely corresponding with histology, that reveal micro-scale mechanical contrast in human breast and lymph node tissues. We use phase-sensitive, three-dimensional optical coherence tomography (OCT) to probe the nanometer-to-micrometer-scale axial displacements in tissues induced by compressive loading. Optical coherence micro- elastography incorporates common-path interferometry, weighted averaging of the complex OCT signal and weighted least-squares regression. Using three-dimensional phase unwrapping, we have increased the maximum detectable strain eleven-fold over no unwrapping and the minimum detectable strain is 2.6 με. We demonstrate the potential of mechanical over optical contrast for visualizing micro-scale tissue structures in human breast cancer pathology and lymph node morphology. © 2014 Optical Society of America.
    Original languageEnglish
    Pages (from-to)2113-2124
    JournalBiomedical Optics Express
    Volume5
    Issue number7
    Early online date9 Jun 2014
    DOIs
    Publication statusPublished - Jul 2014

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