Online oxygen kinetic isotope effects using membrane inlet mass spectrometry can differentiate between oxidases for mechanistic studies and calculation of their contributions to oxygen consumption in whole tissues

M. Cheah; Harvey Millar; R.C. Myers; D. Day; J.P. Roth; W. Hillier; M.R. Badger

doi:10.1021/ac501086n

Online oxygen kinetic isotope effects using membrane inlet mass spectrometry can differentiate between oxidases for mechanistic studies and calculation of their contributions to oxygen consumption in whole tissues

M. Cheah, Harvey Millar, R.C. Myers, D. Day, J.P. Roth, W. Hillier, M.R. Badger

ARC Centre for Plant Energy Biology

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

The reduction chemistry of molecular oxygen underpins the energy metabolism of multicellular organisms, liberating free energy needed to catalyze a plethora of enzymatic reactions. Measuring the isotope signatures of 16O and 18O during O2 reduction can provide insights into both kinetic and equilibrium isotope effects. However, current methods to measure O2 isotope signatures are time-consuming and disruptive. This paper describes the application of membrane inlet mass spectrometry to determine the oxygen isotope discrimination of a range of O 2-consuming reactions, providing a rapid and convenient method for determining these values. A survey of oxygenase and oxidase reactions provides new insights into previously uncharacterized amino acid oxidase enzymes. Liquid and gas phase measurements show the ease of assays using this approach for purified enzymes, biological extracts and intact tissues. © 2014 American Chemical Society.

Original language	English
Pages (from-to)	5171-5178
Journal	Analytical Chemistry
Volume	86
Issue number	10
DOIs	https://doi.org/10.1021/ac501086n
Publication status	Published - 2014

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Cheah, M., Millar, H., Myers, R. C., Day, D., Roth, J. P., Hillier, W., & Badger, M. R. (2014). Online oxygen kinetic isotope effects using membrane inlet mass spectrometry can differentiate between oxidases for mechanistic studies and calculation of their contributions to oxygen consumption in whole tissues. Analytical Chemistry, 86(10), 5171-5178. https://doi.org/10.1021/ac501086n

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title = "Online oxygen kinetic isotope effects using membrane inlet mass spectrometry can differentiate between oxidases for mechanistic studies and calculation of their contributions to oxygen consumption in whole tissues",

abstract = "The reduction chemistry of molecular oxygen underpins the energy metabolism of multicellular organisms, liberating free energy needed to catalyze a plethora of enzymatic reactions. Measuring the isotope signatures of 16O and 18O during O2 reduction can provide insights into both kinetic and equilibrium isotope effects. However, current methods to measure O2 isotope signatures are time-consuming and disruptive. This paper describes the application of membrane inlet mass spectrometry to determine the oxygen isotope discrimination of a range of O 2-consuming reactions, providing a rapid and convenient method for determining these values. A survey of oxygenase and oxidase reactions provides new insights into previously uncharacterized amino acid oxidase enzymes. Liquid and gas phase measurements show the ease of assays using this approach for purified enzymes, biological extracts and intact tissues. {\textcopyright} 2014 American Chemical Society.",

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Cheah, M, Millar, H, Myers, RC, Day, D, Roth, JP, Hillier, W & Badger, MR 2014, 'Online oxygen kinetic isotope effects using membrane inlet mass spectrometry can differentiate between oxidases for mechanistic studies and calculation of their contributions to oxygen consumption in whole tissues', Analytical Chemistry, vol. 86, no. 10, pp. 5171-5178. https://doi.org/10.1021/ac501086n

Online oxygen kinetic isotope effects using membrane inlet mass spectrometry can differentiate between oxidases for mechanistic studies and calculation of their contributions to oxygen consumption in whole tissues. / Cheah, M.; Millar, Harvey; Myers, R.C. et al.
In: Analytical Chemistry, Vol. 86, No. 10, 2014, p. 5171-5178.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

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AU - Cheah, M.

AU - Millar, Harvey

AU - Myers, R.C.

AU - Day, D.

AU - Roth, J.P.

AU - Hillier, W.

AU - Badger, M.R.

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AB - The reduction chemistry of molecular oxygen underpins the energy metabolism of multicellular organisms, liberating free energy needed to catalyze a plethora of enzymatic reactions. Measuring the isotope signatures of 16O and 18O during O2 reduction can provide insights into both kinetic and equilibrium isotope effects. However, current methods to measure O2 isotope signatures are time-consuming and disruptive. This paper describes the application of membrane inlet mass spectrometry to determine the oxygen isotope discrimination of a range of O 2-consuming reactions, providing a rapid and convenient method for determining these values. A survey of oxygenase and oxidase reactions provides new insights into previously uncharacterized amino acid oxidase enzymes. Liquid and gas phase measurements show the ease of assays using this approach for purified enzymes, biological extracts and intact tissues. © 2014 American Chemical Society.

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JO - Analytical Chemistry

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