Oncostatin M stimulates proliferation, induces collagen production and inhibits apoptosis of human lung fibroblasts

A.K. Scaffidi, S.E. Mutsaers, Y.P. Moodley, R.J. Mcanulty, G.J. Laurent, Philip Thompson, [No Value] Knight Da

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Abstract

1 Oncostatin M (OSM), a member of the interleukin-6 (IL-6) cytokine family, acts on a variety of cells and elicits diversified biological responses, suggesting potential roles in the regulation of cell survival, differentiation and proliferation.2 We have examined the effect of OSM on the regulation of human lung fibroblast proliferation, collagen production and spontaneous apoptosis. The proliferative effects of OSM (0.5 - 100 ng ml(-1)) were assessed using a MTS assay as well as [H-3]-thymidine incorporation and cell counts at 24 and 48 h. Hydroxyproline was measured as an index of procollagen production by high pressure liquid chromotography (HPLC). Apoptosis was determined by annexin staining.3 OSM enhanced the mitotic activity of lung fibroblasts in a time and dose dependent manner. Maximum proliferation of 57% above control was observed after incubation for 48 h with 2 ng ml(-1) OSM (P
Original languageEnglish
Pages (from-to)793-801
JournalBritish Journal of Pharmacology
Volume136
DOIs
Publication statusPublished - 2002

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