TY - JOUR
T1 - Oncostatin M stimulates proliferation, induces collagen production and inhibits apoptosis of human lung fibroblasts
AU - Scaffidi, A.K.
AU - Mutsaers, S.E.
AU - Moodley, Y.P.
AU - Mcanulty, R.J.
AU - Laurent, G.J.
AU - Thompson, Philip
AU - Knight Da, [No Value]
PY - 2002
Y1 - 2002
N2 - 1 Oncostatin M (OSM), a member of the interleukin-6 (IL-6) cytokine family, acts on a variety of cells and elicits diversified biological responses, suggesting potential roles in the regulation of cell survival, differentiation and proliferation.2 We have examined the effect of OSM on the regulation of human lung fibroblast proliferation, collagen production and spontaneous apoptosis. The proliferative effects of OSM (0.5 - 100 ng ml(-1)) were assessed using a MTS assay as well as [H-3]-thymidine incorporation and cell counts at 24 and 48 h. Hydroxyproline was measured as an index of procollagen production by high pressure liquid chromotography (HPLC). Apoptosis was determined by annexin staining.3 OSM enhanced the mitotic activity of lung fibroblasts in a time and dose dependent manner. Maximum proliferation of 57% above control was observed after incubation for 48 h with 2 ng ml(-1) OSM (P
AB - 1 Oncostatin M (OSM), a member of the interleukin-6 (IL-6) cytokine family, acts on a variety of cells and elicits diversified biological responses, suggesting potential roles in the regulation of cell survival, differentiation and proliferation.2 We have examined the effect of OSM on the regulation of human lung fibroblast proliferation, collagen production and spontaneous apoptosis. The proliferative effects of OSM (0.5 - 100 ng ml(-1)) were assessed using a MTS assay as well as [H-3]-thymidine incorporation and cell counts at 24 and 48 h. Hydroxyproline was measured as an index of procollagen production by high pressure liquid chromotography (HPLC). Apoptosis was determined by annexin staining.3 OSM enhanced the mitotic activity of lung fibroblasts in a time and dose dependent manner. Maximum proliferation of 57% above control was observed after incubation for 48 h with 2 ng ml(-1) OSM (P
U2 - 10.1038/sj.bjp.0704769
DO - 10.1038/sj.bjp.0704769
M3 - Article
C2 - 12086989
SN - 0007-1188
VL - 136
SP - 793
EP - 801
JO - British Journal of Pharmacology
JF - British Journal of Pharmacology
ER -