Almost 100 years after the publication of Thompson's seminal book "On growth and form", the study of animal morphology is becoming again central to biology. This is also thanks to the development of powerful computerized quantitative methods for statistical shape analysis collectively known as geometric morphometrics (GM).GMwas announced as a revolution just two decades ago. The "revolution" is now a standard tool in numerical analyses of phenotypic variation in mammals and other organisms. Hundreds of studies are published every year that take advantage of GM (e.g., more than 800 entries in Google Scholar only for 2012). We celebrate the 20th anniversary of the "revolution in morphometrics" (Rohlf and Marcus, 1993, p. 129) with the publication of a "Yellow Book", a special issue of Hystrix dedicated to Evolutionary Morphometrics and Virtual Morphology. A series of 14 papers by leading morphometricians summarizes the main achievements of GM (surface methods, "comparative shape analysis", phenotypic trajectories quantification, modularity/ integration, the use of R in morphometrics), describes its most innovative developments (ecometrics, eigensound analysis, biomechanical GM), and discusses common misunderstandings of well extablished methods (visualization of shape differences). Besides celebrating the success of statistical shape analysis in biology, this issue aims at introducing to GM readers unfamiliar with or intimidated by its strong numerical background. This is why, as Editors, we asked all contributors to provide concise and accurate but also clear and simple descriptions of techniques and applications. We hope that we succeeded in this aim, and wish that this Yellow Book may help to tighten the connection between biologists and statisticians for a truly "biological" GM. © 2013 Associazione Teriologica Italiana.